Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Corredor, E.; Raska, I.; Testillano, P. S.; Risueno, M.C.(Elsevier, 2011-05-15)
After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. ...
Sohrabi, Sara; Abdollahi, Mohammad Reza; Mirzaie-Asl, Asghar; Koulaei, Hassan Ebrahimi; Aghaeezadeh, Mohsen; Seguí-Simarro, Jose M.(Springer-Verlag, 2021-08)
[EN] Induction of gynogenesis through ovule culture is a valuable tool to produce haploid and doubled haploid plants in sugar beet (Beta vulgaris L.). However, there is still large room for refining the method. In this ...
Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Parra Vega, Verónica; Gonzalez Garcia, Beatriz(Springer Verlag (Germany), 2011-05)
[EN] Tomato, eggplant, and pepper are three solanaceous crops of outstanding importance worldwide. For hybrid seed production in these species, a fast and cheap method to obtain pure (homozygous) lines is a priority. ...
Fortes, A.; Costa, J.; Santos, F.; Seguí-Simarro, Jose M.; Palme, K.; Altabella, T.; Tiburcio, A.F.; Pais, M.A.(Taylor & Francis, 2011-02)
Hop (Humulus lupulus L.) is an economically important plant species used in beer production and as a health-promoting medicine. hop internodes develop upon stress treatments organogenic nodules which can be used for genetic ...
Parra Vega, Verónica(Universitat Politècnica de València, 2015-03-31)
La embriogénesis derivada de microsporas es la ruta androgénica más
empleada para la obtención de individuos dobles haploides (DHs), 100%
homocigotos, que pueden ser utilizados como líneas puras. Este proceso es ...
Asadi, Abouzar; Zebarjadi, Alireza; Abdollahi, Mohammad Reza; Seguí-Simarro, Jose M.(Springer-Verlag, 2018)
[EN] Cucumber is one of the most important vegetable crops worldwide, which makes it a good candidate to produce doubled haploid (DH) lines to accelerate plant breeding. Traditionally, these approaches involved induction ...
Seguí Simarro, José María(Editorial Universitat Politècnica de València, 2011-09-30)
La biología reproductiva de las plantas engloba todos los procesos que permiten a un organismo vegetal tener descendencia, sea por vía sexual o asexual. El conocimiento de estos procesos es esencial para poder sacar provecho ...
Rivas Sendra, Alba(Universitat Politècnica de València, 2017-07-20)
Androgenesis induction is an experimental procedure by which microspores are diverted from their original gametophytic pathway towards embryogenesis by applying specific stresses in vitro. It allows for the production of ...
Calabuig-Serna, Antonio; Mir Moreno, Ricardo; Arjona, Paloma; Seguí-Simarro, Jose M.(Frontiers Media SA, 2023-03-31)
[EN] Free calcium (Ca2+) is a pivotal player in different in vivo and in vitro morphogenic
processes. In the induction of somatic embryogenesis, its role has been
demonstrated in different species. In carrot, however, this ...
Calabuig-Serna, Antonio; Mir Moreno, Ricardo; Seguí-Simarro, Jose M.(MDPI, 2023-03)
[EN] In this work, we studied the induction of somatic embryogenesis in Arabidopsis using IZEs as explants. We characterized the process at the light and scanning electron microscope level and studied several specific ...
Campos Vega, Manuel(Universitat Politècnica de València, 2016-04-22)
[EN] This work is part of a research aimed to obtain a line of high androgenic response, to use as model in different studies related to the androgenesis. We used different doubled haploid lines (Solanum melongena) in order ...
García Fortea, Edgar(Universitat Politècnica de València, 2015-09-04)
[ES] La puesta a punto de métodos eficientes de obtención de individuos doble haploides (DHs) es uno de los objetivos principales de muchas empresas empresas de mejora genética, pues permiten obtener líneas puras de forma ...
Camacho-Fernández, Carolina; Seguí-Simarro, Jose M.; Mir Moreno, Ricardo; Boutilier, Kim; Corral-Martínez, Patricia(Frontiers Media SA, 2021-10-06)
[EN] Microspore cultures generate a heterogeneous population of embryogenic structures that can be grouped into highly embryogenic structures [exine-enclosed (EE) and loose bicellular structures (LBS)] and barely embryogenic ...
Sanz-Barrio, R.; Fernández-San Millán, A.; Carballeda, J.; Corral Martínez, Patricia; Seguí-Simarro, Jose M.; Farran, I.(Oxford University Press (OUP): Policy B - Oxford Open Option A, 2012)
Thioredoxins (Trxs) are ubiquitous disulphide reductases that play important roles in the redox regulation of many cellular processes. However, some redox-independent functions, such as chaperone activity, have also been ...
Prohens Tomás, Jaime; Plazas Ávila, María de la O; Raigón Jiménez, Mª Dolores; Seguí-Simarro, José M.; Stommel, John R.; Vilanova Navarro, Santiago(Springer Verlag (Germany), 2012)
Common (Solanum melongena L.) and scarlet (S. aethiopicum L.) eggplants are cultivated for their fruits and form part of the same genepool. We have studied plant and fruit characteristics, pollen viability and seed set, ...
Camacho-Fernández, Carolina; Hervás, David; Rivas-Sendra, Alba; Marín, Mª Pilar; Seguí-Simarro, Jose M.(Springer (Biomed Central Ltd.), 2018)
[EN] Background: For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A
typical example of this is isolated microspore culture, where specific cell densities ...
Tur Giménez, Laura(Universitat Politècnica de València, 2020-10-05)
[ES] La embriogénesis de microsporas consiste en desviar in vitro la ruta normal de desarrollo gametofítico de las microsporas para formar un embrión a partir del cual se obtendrán plantas haploides o dobles haploides tras ...
Camacho Fernández, Carolina(Universitat Politècnica de València, 2016-03-11)
[EN] In isolated microspores cultures, cellular density is a key factor in the response to androgenesis induction. Traditionally, counting chambers have been used to adjust the initial density of microspores, but other ...
Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Kouassi, Brice; Prohens Tomás, Jaime; Gramazio, Pietro; Kouassi, Abou; Vilanova Navarro, Santiago; Galán-Ávila, Alberto; Herraiz García, Francisco Javier; Kouassi, Auguste; Seguí-Simarro, Jose M.; Plazas Ávila, María de la O(Elsevier, 2016)
[EN] Introgression breeding can contribute to broadening the genetic background of eggplant (Solanum melongena). We used six eggplant varieties and 44 interspecific hybrids between these eggplant accessions and 10 accessions ...