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Listar por autor "Aragonés Blasco, Verónica"

Mostrando ítems 1-9 de 9

A viroid-derived system to produce large amounts of recombinant RNA in Escherichia coli

DAROS ARNAU, JOSE ANTONIO; Aragones, V; Cordero-Cucart, María Teresa (Nature Publishing Group, 2018)

[EN] Viruses have been engineered into useful biotechnological tools for gene therapy or to induce the synthesis of products of interest, such as therapeutic proteins and vaccines, in animal and fungal cells, bacteria or ...
A Watermelon mosaic virus clone tagged with the yellow visual maker phytoene synthase facilitates scoring infectivity in melon breeding programs

Aragones, V; Pérez De Castro, Ana María; Cordero, Teresa; Cebolla Cornejo, Jaime; López Del Rincón, Carmelo; Picó Sirvent, María Belén; DAROS ARNAU, JOSE ANTONIO (Springer-Verlag, 2019)
Carotenoid fortification of zucchini fruits using a viral RNA vector

Fakhreddine-Houhou, Houhou; Martí-Botella, Mari Carmen; Cordero-Cucart, María Teresa; Aragones, V; Sáez-Sánchez, Cristina; Cebolla Cornejo, Jaime; Pérez De Castro, Ana María; Rodriguez-Concepcion, Manuel; Picó Sirvent, María Belén; Daròs, José-Antonio (John Wiley & Sons, 2022-05)

[EN] Background Carotenoids are health-promoting metabolites in livestock and human diets. Some important crops have been genetically modified to increase their content. Although the usefulness of transgenic plants to ...
Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

Uranga-Ruiz De Eguino, Mireia; Aragones, V; Selma García, Sara; Vázquez-Vilar, Marta; Orzáez Calatayud, Diego Vicente; Daròs, José-Antonio (Blackwell Publishing, 2021-04)

[EN] Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas ...
Efficient production of saffron crocins and picrocrocin in Nicotiana benthamiana using a virus-driven system

Martí, Maricarmen; Diretto, Gianfranco; Aragones, V; Frusciante, Sarah; Ahrazem, Oussama; Gómez-Gómez, Lourdes; Daròs, José-Antonio (Elsevier, 2020-09)

[EN] Crocins and picrocrocin are glycosylated apocarotenoids responsible, respectively, for the color and the unique taste of the saffron spice, known as red gold due to its high price. Several studies have also shown the ...
Eggplant latent viroid is located in the chloroplasts and nuclei of eggplant infected cells

Eiras, Marcelo; Aragones, V.; Marqués, Jorge; Gómez-Jiménez, María Dolores; Daròs, José-Antonio (Springer (Biomed Central Ltd.), 2024-10-15)

[EN] Viroids that belong to genera Avsunviroid and Pelamovirod (family Avsunviroidae) replicate and accumulate in the chloroplasts of infected cells. In this report, we confirmed by RNA in situ hybridization using ...
Production of Saffron Apocarotenoids in Nicotiana benthamiana Plants Genome-Edited to Accumulate Zeaxanthin Precursor

Demurtas, Olivia Costantina; Sulli, Maria; Ferrante, Paola; Mini, Paola; Aragones, V.; Daròs, José-Antonio; Marti, Maricarmen; Giuliano, Giovanni (MDPI AG, 2023-06)

[EN] Crocins are glycosylated apocarotenoids with strong coloring power and anti-oxidant, anticancer, and neuro-protective properties. We previously dissected the saffron crocin biosynthesis pathway, and demonstrated that ...
RNA Virus-Mediated Gene Editing for Tomato Trait Breeding

Uranga-Ruiz De Eguino, Mireia; Aragones, V.; García, Arcadio; Mirabel, Sophie; Gianoglio, Silvia; Presa Castro, Silvia; GRANELL RICHART, ANTONIO; Pasin, Fabio; Daròs, José-Antonio (Oxford University Press, 2024-01-10)

[EN] Virus-induced genome editing (VIGE) leverages viral vectors to deliver CRISPR-Cas components into plants for robust and flexible trait engineering. We describe here a VIGE approach applying an RNA viral vector based ...
Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors

Aragones, V; Aliaga, F; Daròs, José-Antonio; Pasin, F (John Wiley & Sons, 2022-07)

[EN] Viral vectors provide a quick and effective way to express exogenous sequences in eukaryotic cells and to engineer eukaryotic genomes through the delivery of CRISPR/Cas components. Here, we presentJoinTRV, an improved ...