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Listar por autor "Selma García, Sara"

Mostrando ítems 1-9 de 9

A copper switch for inducing CRISPR/Cas9-based transcriptional activation tightly regulates gene expression in Nicotiana benthamiana

García-Pérez, Elena; Diego-Martín, Borja; Quijano-Rubio, Alfredo; Moreno-Giménez, Elena; Selma, Sara; Orzáez Calatayud, Diego Vicente; Vázquez-Vilar, Marta (Springer (Biomed Central Ltd.), 2022-03-24)

[EN] Background CRISPR-based programmable transcriptional activators (PTAs) are used in plants for rewiring gene networks. Better tuning of their activity in a time and dose-dependent manner should allow precise control ...
Custom-made design of metabolite composition in N. benthamiana leaves using CRISPR activators

Selma, Sara; Sanmartín, Neus; Espinosa-Ruiz, Ana; Gianoglio, Silvia; López-Gresa, María Pilar; Vázquez-Vilar, Marta; Flors, Victor; GRANELL RICHART, ANTONIO; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-08)

[EN] Transcriptional regulators based on CRISPR architecture expand our ability to reprogramme endogenous gene expression in plants. One of their potential applications is the customization of plant metabolome through the ...
Development of CRISPR-based programmable transcriptional regulators and their applications in plants

Selma García, Sara (Universitat Politècnica de València, 2022-09-01)

[ES] La Biología Sintética de Plantas tiene como objetivo rediseñar las plantas para que adquieran características y funcionalidades novedosas a través de circuitos reguladores ortogonales. Para lograr este objetivo, se ...
Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

Uranga-Ruiz De Eguino, Mireia; Aragones, V; Selma García, Sara; Vázquez-Vilar, Marta; Orzáez Calatayud, Diego Vicente; Daròs, José-Antonio (Blackwell Publishing, 2021-04)

[EN] Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR) and CRISPR-associated proteins (Cas) have revolutionized genome editing in many organisms, including plants. Most CRISPR-Cas ...
Estudio de la regulación transcripcional del promotor de la proteína metalotioneína de tipo 2B mediante el sistema CRISPR/Cas9 en Nicotiana benthamiana

Ajenjo Bauzá, María (Universitat Politècnica de València, 2018-09-14)

[EN] The discovery of CRISPR/Cas system has meant a revolution in the synthetic biology field. Among all its applications, it could be found the genomic engineering or programmable transcriptional modulation. In this work, ...
Functionality optimization of CRISPR-Cas13d as a new repressor system in Nicotiana Benthamiana

Freire Alarcón, Jacqueline Belén (Universitat Politècnica de València, 2021-02-12)

[ES] La tecnología CRISPR / Cas es una plataforma valiosa que ha proporcionado herramientas eficientes para la biología sintética de plantas. Varios estudios han intentado dirigirse al ARN empleando sistemas establecidos ...
GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expressionin Plants

Moreno-Giménez, Elena; Selma, Sara; Calvache-Román, Camilo Alberto; Orzáez Calatayud, Diego Vicente (American Chemical Society, 2022-08-31)

[EN] Programmable transcriptional factors based on the CRISPR architecture are becoming commonly used in plants for endogenous gene regulation. In plants, a potent CRISPR tool for gene induction is the so-called dCasEV2.1 ...
Strong and tunable anti-CRISPR/Cas activities in plants

Calvache-Román, Camilo Alberto; Selma, Sara; Vázquez-Vilar, Marta; Uranga, Mireia; Fernández-Del-Carmen, Asun; Daròs, José-Antonio; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-02)

[EN] CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This ...
Synthetic transcriptional repressors design based on the CRISPR-Cas technology in N. benthamiana

Salazar Sarasúa, Blanca (Universitat Politècnica de València, 2020-02-12)

[EN] Deactivated versions of Cas proteins like dCas9 and dCas12a open new posibilities for plant synthetic biology in the realm of negative transcriptional regulation. Here we describe two repression strategies tested ...