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Anti-CRISPR protein AcrIIA4 as a novel tool to control CRISPR/Cas9-mediated gene editing and activation in Nicotiana benthamiana

Calvache Román, Camilo Alberto (Universitat Politècnica de València, 2021-02-12)

[ES] La tecnología CRISPR-Cas9 se ha convertido en una herramienta esencial en la biotecnología de plantas, cambiando nuestra capacidad para manipular genomas y estudiar redes moleculares gracias a las posibilidades ofrecidas ...
GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for Fine-Tuned Recombinant Gene Expressionin Plants

Moreno-Giménez, Elena; Selma, Sara; Calvache-Román, Camilo Alberto; Orzáez Calatayud, Diego Vicente (American Chemical Society, 2022-08-31)

[EN] Programmable transcriptional factors based on the CRISPR architecture are becoming commonly used in plants for endogenous gene regulation. In plants, a potent CRISPR tool for gene induction is the so-called dCasEV2.1 ...
Strong and tunable anti-CRISPR/Cas activities in plants

Calvache-Román, Camilo Alberto; Selma, Sara; Vázquez-Vilar, Marta; Uranga, Mireia; Fernández-Del-Carmen, Asun; Daròs, José-Antonio; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-02)

[EN] CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This ...

Mostrando ítems 1-3 de 3