[EN] The development of double haploids (DHs) is a straightforward path for obtaining pure lines but has multiple bottlenecks. Among them is the determination of the optimal stage of pollen induction for androgenesis. In ...
Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Parra Vega, Verónica; Gonzalez Garcia, Beatriz(Springer Verlag (Germany), 2011-05)
[EN] Tomato, eggplant, and pepper are three solanaceous crops of outstanding importance worldwide. For hybrid seed production in these species, a fast and cheap method to obtain pure (homozygous) lines is a priority. ...
Rivas Sendra, Alba(Universitat Politècnica de València, 2017-07-20)
Androgenesis induction is an experimental procedure by which microspores are diverted from their original gametophytic pathway towards embryogenesis by applying specific stresses in vitro. It allows for the production of ...
Campos Vega, Manuel(Universitat Politècnica de València, 2016-04-22)
[EN] This work is part of a research aimed to obtain a line of high androgenic response, to use as model in different studies related to the androgenesis. We used different doubled haploid lines (Solanum melongena) in order ...
Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Mir Moreno, Ricardo; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(MDPI AG, 2021-07)
[EN] This review compiles the most relevant advances made in the production of doubled haploid plants in eggplant, the application of doubled haploid lines in breeding programs, and the future perspectives for the development ...
Salas Aragon, Pablo; Prohens Tomás, Jaime; Seguí-Simarro, José M.(Springer Verlag (Germany), 2011)
Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 ...
In plant breeding, androgenic doubled haploids represent powerful tools to save time and resources for pure line generation. While in many species efficient protocols are known, in tomato (Solanum lycopersicum), the knowledge ...
Salas Aragon, Pablo; Rivas Sendra, Alba; Prohens Tomás, Jaime; Seguí-Simarro, José M.(Springer Verlag (Germany), 2012)
In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly ...
Cerezuela Martínez, Alicia(Universitat Politècnica de València, 2020-09-07)
[ES] La obtención de líneas puras es un proceso de gran relevancia para la industria agronómica, pues a partir de su cruce, se pueden obtener híbridos comerciales de mayor rendimiento y homogeneidad en la producción.
La ...
Hoveida, Z.S.; Abdollahi, Mohammad Reza; Mirzaie-Asl, Asghar; Moosavi, Sayyed Saeed; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] Anther culture can be used as a powerful tool to produce doubled haploid (DH) lines in medicinal plants, thus accelerating breeding programs. In the particular case of borage (Borago officinalis L.), a method to produce ...