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Listar por palabra clave "Artificial microRNA"

Mostrando ítems 1-8 de 8

Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection

Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio (Wiley, 2016)

[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence endogenous transcripts as well as viral RNAs ...
Downregulation of Tomato STEROL GLYCOSYLTRANSFERASE 1 Perturbs Plant Development and Facilitates Viroid Infection

Cisneros, Adriana E.; Lisón, Purificación; Campos, Laura; López-Tubau, Joan Manel; Altabella, Teresa; Ferrer, Albert; Daròs, José-Antonio; CARBONELL, ALBERTO (Oxford University Press, 2022)

[EN] Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer tomato plants highly resistant to PSTVd and ...
Efecto del apareamiento de los nucleótidos del precursor shc-MIR390 sobre la acumulación de microRNAs artificiales derivados del mismo

García Cano, Pedro José (Universitat Politècnica de València, 2024-02-14)

[ES] El silenciamiento génico mediado RNA o RNA de interferencia (RNAi) es un mecanismo de regulación de la expresión génica tanto en células animales como vegetales. El RNAi está mediado por pequeños RNAs (sRNAs) no ...
Fast-forward generation of effective artificial small RNAs for enhanced antiviral defense in plants

CARBONELL, ALBERTO; Carrington, James C.; Daros Arnau, Jose Antonio (Smart Science and Technology, 2016-01-12)

[EN] Artificial small RNAs (sRNAs) are short ≈21-nt non-coding RNAs engineered to inactivate sequence complementary RNAs. In plants, they have been extensively used to silence cellular transcripts in gene function analyses ...
Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors

CARBONELL, ALBERTO; Fahlgren, Noah; Mitchell, Skyler; Cox, Kevin L., Jr.; Reilly, Kevin C.; Mockler, Todd C.; Carrington, James C. (Blackwell Publishing, 2015-06)

[EN] Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective ...
P-SAMS: a web suite for plant artificial microRNA and synthetic trans-acting small interfering RNA design

Fahlgren, Noah; Hill, Steven T.; Carrington, James C.; CARBONELL, ALBERTO (Oxford University Press, 2016-01-01)

[EN] The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syntasiRNAs) for efficient and specific ...
RNAi tools for controlling viroid diseases

CARBONELL, ALBERTO (Elsevier, 2022-05)

[EN] Viroids are small (250-400 nucleotides), single-stranded, circular RNAs without protein-coding capacity that infect a large number of ornamental and crop plant species, causing high economic losses worldwide. Strategies ...
Systemic silencing of an endogenous plant gene by two classes of mobile 21-nucleotide artificial small RNAs

Cisneros, Adriana E.; De la Torre-Montaña, Ainhoa; CARBONELL, ALBERTO (Blackwell Publishing, 2022-05)

[EN] Artificial small RNAs (art-sRNAs) are 21-nucleotide small RNAs (sRNAs) computationally designed to silence plant genes or pathogenic RNAs with high efficacy and specificity. They are typically produced in transgenic ...