Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Corredor, E.; Raska, I.; Testillano, P. S.; Risueno, M.C.(Elsevier, 2011-05-15)
After a stress treatment, in vitro-cultured pollen changes its normal gametophytic developmental pathway towards embryogenesis producing multicellular embryos from which, finally, haploid and double haploid plants develop. ...
Tur Giménez, Laura(Universitat Politècnica de València, 2020-10-05)
[ES] La embriogénesis de microsporas consiste en desviar in vitro la ruta normal de desarrollo gametofítico de las microsporas para formar un embrión a partir del cual se obtendrán plantas haploides o dobles haploides tras ...
Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis ...
Corral Martínez, Patricia; Seguí-Simarro, José M.(Springer Verlag (Germany), 2012)
Production of doubled haploids (DHs) through androgenesis induction is an important biotechnological tool for plant breeding. In some species, DHs are efficiently obtained through embryogenesis from isolated microspore ...
Salas Aragon, Pablo; Prohens Tomás, Jaime; Seguí-Simarro, José M.(Springer Verlag (Germany), 2011)
Anther culture is a convenient technique to obtain androgenic haploid and doubled haploid (DH) plants. In common eggplant (Solanum melongena), this technique has been used to develop DH pure lines for producing uniform F1 ...
Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M.(Frontiers Media, 2015-02)
[EN] The change in developmental fate of microspores reprogrammed toward embryogenesis
is a complex but fascinating experimental system where microspores undergo dramatic
changes derived from the developmental switch. ...
[EN] Doubled haploid (DH) technology allows for
the production of pure lines, useful for plant breeding,
through a one-generation procedure that reduces considerably
the time and resources needed to produce them.
...
Eshaghi, Zara Chardoli; Abdollahi, Mohammad Reza; Moosavi, Sayyed Saeed; Deljou, Ali; Seguí-Simarro, Jose M.(Springer Verlag (Germany), 2015-08)
[EN] Borage (Borago officinalis L.) is an important medicinal plant with different culinary, pharmaceutical and industrial properties. Unfortunately, there are no published reports on the establishment of protocols to ...
Parra Vega, Verónica; Corral Martínez, Patricia; Rivas-Sendra, Alba; Seguí-Simarro, Jose M.(Frontiers Media, 2015-11-25)
The induction of microspore embryogenesis produces dramatic changes in different aspects of the cell physiology and structure. Changes at the cell wall level are among the most intriguing and poorly understood. In this ...
Salas Aragon, Pablo; Rivas Sendra, Alba; Prohens Tomás, Jaime; Seguí-Simarro, José M.(Springer Verlag (Germany), 2012)
In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly ...
Camacho Fernández, Carolina(Universitat Politècnica de València, 2021-03-08)
[ES] Los dobles haploides son una gran herramienta para la mejora genética de híbridos debido a que se puede alcanzar la homocigosis completa en una sola generación. Entre las técnicas usadas para obtener estas plantas, ...
Calabuig Serna, Antonio(Universitat Politècnica de València, 2023-09-06)
[ES] El calcio (Ca2+) es un catión esencial que juega un papel fundamental en todos los organismos vivos. Desde el punto de vista funcional, el Ca2+ actúa como un segundo mensajero que regula distintos procesos celulares. ...
Corral Martínez, Patricia; Parra Vega, Verónica; Seguí-Simarro, Jose M.(Oxford University Press (OUP): Policy B - Oxford Open Option A, 2013)
[EN] Induction of embryogenesis from isolated microspore cultures is a complex experimental system where microspores undergo dramatic changes in developmental fate. After ~40 years of application of electron microscopy to ...
Corral Martínez, Patricia(Universitat Politècnica de València, 2011-07-28)
La producción de doble haploides a través del cultivo de microsporas aisladas es una
alternativa para obtener líneas puras mucho más eficaz que las técnicas de mejora genética
clásica. Esta técnica presenta las ventajas ...
Mir Moreno, Ricardo; Vicente, Oscar(University of Agronomic Sciences and Veterinary Medicine of Bucharest, 2021)
[EN] Pollen, the male gametophyte of higher plants, represents an extremely interesting, and peculiar, experimental system for the study of diverse aspects of plant biology, apart from the - 0bvious - research on gametophytic ...
Hoveida, Z.S.; Abdollahi, Mohammad Reza; Mirzaie-Asl, Asghar; Moosavi, Sayyed Saeed; Seguí-Simarro, Jose M.(Springer-Verlag, 2017)
[EN] Anther culture can be used as a powerful tool to produce doubled haploid (DH) lines in medicinal plants, thus accelerating breeding programs. In the particular case of borage (Borago officinalis L.), a method to produce ...
Calabuig-Serna, Antonio; Camacho-Fernández, Carolina; Mir Moreno, Ricardo; Porcel, R; Carrera, Esther; LOPEZ DIAZ, ISABEL; Seguí-Simarro, Jose M.(Springer-Verlag, 2022-03)
[EN] In eggplant microspore embryogenesis, embryos are produced and then transformed into undifferentiated calli, instead of developing as true embryos. This is the main current bottleneck that precludes this process from ...
Corral Martínez, Patricia; Seguí-Simarro, Jose M.(Springer Verlag (Germany), 2014-02)
Microspore embryogenesis is an inducible pathway interesting from basic and applied perspectives. For plant breeding, it is a powerful tool to produce doubled haploids, useful as pure lines. The most efficient way to produce ...
Parra Vega, Verónica; Renau Morata, Begoña; Sifres Cuerda, Alicia Gemma; Seguí Simarro, José María(Springer Verlag (Germany), 2013-03)
Production of doubled haploids (DHs) is a convenient tool to obtain pure lines for breeding purposes. Until now, the easiest and most useful approach to obtain pepper DHs is via anther culture. However, this method has an ...