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Listar por palabra clave "Polymerase chain reaction"

Mostrando ítems 1-13 de 13

Assessment of parental contributions to fast- and slow-growing progenies in the sea bream Sparus aurata L. using a new multiplex PCR

Borrell, Yaisel J.; Gallego Albiach, Victor; García Fernández, Carmen; Mazzeo ., Ilaria; Pérez Igualada, Luz María; Asturiano Nemesio, Juan Francisco; Carleos, Carlos E.; Vázquez, Emilia; Sánchez, José A.; Blanco, Gloria (Elsevier, 2011-04-04)

Molecular tools to assist breeding programs in the gilthead sea bream (Sparus aurata L.) are scarce. A new multiplex PCR technique (OVIDORPLEX), which amplifies nine known microsatellite markers, was developed in this work. ...
Carbon nanoparticles in lateral flow methods to detect genes encoding virulence factors of Shiga toxin-producing Escherichia coli

Noguera Murray, Patricia Silvestre; Posthuma-Trumpie, G.A.; van Tuil, M.; van der Wal, F.J.; de Boer, A.; Moers, A.P.H.A.; van Amerongen, A. (Springer Verlag, 2011)

The use of carbon nanoparticles is shown for the detection and identification of different Shiga toxin-producing Escherichia coli virulence factors (vt1, vt2, eae and ehxA) and a 16S control (specific for E. coli) based ...
Chaperone-like properties of tobacco plastid thioredoxins f and m

Sanz-Barrio, R.; Fernández-San Millán, A.; Carballeda, J.; Corral Martínez, Patricia; Seguí-Simarro, Jose M.; Farran, I. (Oxford University Press (OUP): Policy B - Oxford Open Option A, 2012)

Thioredoxins (Trxs) are ubiquitous disulphide reductases that play important roles in the redox regulation of many cellular processes. However, some redox-independent functions, such as chaperone activity, have also been ...
Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages

Jiménez Gasco, María del Mar; Malcolm, Glenna M.; Berbegal Martínez, Mónica; Armengol Fortí, Josep; JIMENEZ DIAZ, RAFAEL (American Phytopathological Society, 2014-06)

Verticillium wilts caused by the soilborne fungus Verticillium dahliae are among the most challenging diseases to control. Populations of this pathogen have been traditionally studied by means of vegetative compatibility ...
Detección de genes de resistencia a antibióticos en carnes de pollo comercializadas en Valencia

Orts Martínez, Samuel (Universitat Politècnica de València, 2024-09-05)

[ES] La OMS (Organización Mundial de la Salud) ha declarado que las resistencias bacterianas a antibióticos suponen uno de los mayores problemas para la salud y seguridad alimentaria. A pesar de ser un fenómeno que ocurre ...
Detection of food-borne pathogens with DNA arrays on disk

Arnandis Chover, Tania; Morais, Sergi; Tortajada-Genaro, Luis Antonio; Puchades, Rosa; Maquieira Catala, Ángel; Berganza, J.; Olabarria, G. (Elsevier, 2012)

A DNA oligonucleotide array for duplex pathogen detection on a DVD platform is developed. The assay involves hybridization of PCR products and optical detection using compact disc technology. Different DNA array constructions ...
Development and application of new molecular markers for analysis of genetic diversity in Verticillium dahliae populations

Berbegal Martínez, Mónica; Garzón, C. D.; Ortega, A.; Armengol Fortí, Josep; Jiménez Díaz, R. M.; Jiménez Gasco, María del Mar (Wiley, 2011-10)

The aim of this study was to develop new polymorphic markers for analysis of genetic diversity in the fungal soilborne plant pathogen Verticillium dahliae. Twelve polymorphic markers (five microsatellites and seven polymorphic ...
Development and evaluation of a real-time PCR assay for quantification of Giardia and Cryptosporidium in sewage samples

Alonso Molina, José Luís; Amoros, Inmaculada; Cañigral Cárcel, Irene (SPRINGER, 2011)

[EN] Cryptosporidium and Giardia are major causes of diarrheal disease in humans worldwide and are major causes of protozoan waterborne diseases. Two DNA TaqMan PCR-based Giardia and Cryptosporidium methods targeting a ...
Evaluación de Campylobacter spp. en carne y vísceras de cerdo y pollo por diferentes métodos microbiológicos y moleculares

Valenzuela Pérez, Ramón Isael (Universitat Politècnica de València, 2019-10-25)

[ES] El análisis microbiológico de Campylobacter spp. en alimentos es una técnica complicada, tanto por las necesidades de microaerofilia, como por la dificultad del aislamiento de estas bacterias en los medios de cultivo, ...
Isolation of a new Mexican strain of Bacillus subtilis with antifungal and antibacterial activities

Basurto Cadena, María Guadalupe Lucía; Vázquez Arista, Manuel; García Jiménez, José; Salcedo Hernandez, R.; Bideshi, D.K.; Barboza Corona, J.E. (Hindawi Publishing Corporation, 2012)

[EN] Although several strains of B. subtilis with antifungal activity have been isolated worldwide, to date there are no published reports regarding the isolation of a native B. subtilis strain from strawberry plants in ...
Multiplex DNA Detection of Food Allergens on a Digital Versatile Disk

Tortajada Genaro, Luis Antonio; Santiago Felipe, Sara; Morais Ezquerro, Sergi Beñat; GABALDÓN, JOSE ANTONIO; Puchades Pla, Rosa; Maquieira Catala, Ángel (American Chemical Society, 2012-01-11)

The development of a DNA microarray method on a digital versatile disk (DVD) is described for the simultaneous detection of traces of hazelnut (Corylus avellana L.), peanut (Arachis hypogaea), and soybean (Glycine max) in ...
Recent Advances on the Multiplex Molecular Detection of Plant Viruses and Viroids

Pallás Benet, Vicente; Sanchez Navarro, Jesús Ángel; James, D. (Frontiers Media SA, 2018)

[EN] Plant viruses are still one of the main contributors to economic losses in agriculture. It has been estimated that plant viruses can cause as much as 50 billion euros loss worldwide, per year. This situation may be ...
Specific detection of viable Listeria monocytogenes in Spanish wastewater treatment plants by Fluorescent In Situ Hybridization and PCR

Moreno Trigos, Mª Yolanda; Ballesteros, Lorena; García Hernández, Jorge; Santiago Cuéllar, Paula; González Pellicer, Ana; Ferrús Pérez, Mª Antonia (IWA Publishing, 2011-10)

Listeria monocytogenes detection in wastewater can be difficult because of the large amount of background microbiota and the presence of viable but non-culturable forms in this environment. The aim of this study was to ...