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Listar por palabra clave "RNA silencing"

Mostrando ítems 1-20 de 34

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A viral suppressor of RNA silencing inhibits ARGONAUTE 1 function by precluding target RNA binding to pre-assembled RISC

Kenesi, Erzsebet; CARBONELL, ALBERTO; Lozsa, Rita; Vertessy, Beata; Lakatos, Lorant (Oxford University Press, 2017-07-27)

[EN] In most eukaryotes, RNA silencing is an adaptive immune system regulating key biological processes including antiviral defense. To evade this response, viruses of plants, worms and insects have evolved viral suppressors ...
Argonaute PIWI domain and microRNA duplex structure contribute to small RNA sorting in Arabidopsis.

Zhang, Xiaoming; Niu, DongDong; CARBONELL, ALBERTO; Wang, Airong; Lee, Angel; Tun, Vinnary; Wang, Zonghua; Carrington, James C.; Chang, Chia-en A.; Jin, Hailing (Nature Publishing Group, 2014-11-16)

[EN] Small RNAs (sRNAs) are loaded into ARGONAUTE (AGO) proteins to induce gene silencing. In plants, the 5¿-terminal nucleotide is important for sRNA sorting into different AGOs. Here we show that microRNA (miRNA) duplex ...
Artificial microRNAs and synthetic trans-acting small interfering RNAs interfere with viroid infection

Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio (Wiley, 2016)

[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence endogenous transcripts as well as viral RNAs ...
Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants

Cisneros, Adriana E.; CARBONELL, ALBERTO (MDPI, 2020-06)

[EN] Artificial small RNAs (art-sRNAs), such as artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are highly specific 21-nucleotide small RNAs designed to recognize and silence ...
Characterisation of differentially expressed trans-acting siRNA (tasiRNAs) facing stress conditions in melon

Cervera Seco, Luis Manuel (Universitat Politècnica de València, 2018-09-18)

[ES] Los trans-acting siRNAs (ta-siRNAs) son una clase de pequeños RNAs (20 a 24 nts) reguladores descritos en plantas. Se producen a partir de regiones especí cas del genoma llamadas genes TAS. Los transcritos derivados ...
Citrus tristeza virus infection induces the accumulation of viral small RNAs (21- 24-nt) mapping preferentially at the 3 -terminal region of the genomic RNA and affects the host small RNA profile

Ruiz Ruiz, Susana; Navarro, Beatriz; Gisel, Andreas; Peña, Leandro; Navarro, Luis; Moreno, Pedro; Di Serio, Francesco; Flores Pedauye, Ricardo (Springer Verlag (Germany), 2011-04)

[EN] To get an insight into the host RNA silencing defense induced by Citrus tristeza virus (CTV) and into the counter defensive reaction mediated by its three silencing suppressors (p25, p20 and p23), we have examined by ...
Citrus tristeza virus: Host RNA Silencing and Virus Counteraction

Ruiz-Ruiz, Susana; Navarro, Beatriz; PEÑA GARCIA, LEANDRO; Navarro, Luis; Moreno, Pedro; Di Serio, Francesco; FLORES PEDAUYE, RICARDO (Springer, 2019)

[EN] To dissect the host RNA silencing response incited by citrus tristeza virus (CTV, genus Closterovirus), a (+) ssRNA of similar to 19300 nt, and the counter reaction deployed by the virus via its three RNA silencing ...
Cytopathic effects incited by viroid RNAs and putative underlying mechanisms

Di Serio, Francesco; De Stradis, Angelo; Delgado Villar, Sonia Guadalupe; Flores Pedauye, Ricardo; Navarro, Beatriz (Frontiers Media, 2013-01-07)

[EN] Viroids are infectious agents identified only in plants so far. In contrast to viruses, the genome of viroids is composed of a tiny circular RNA (250-400 nt) not coding for proteins, but containing in its compact ...
Degradación in vivo de un viroide de replicación nuclear: rutas catalizadas por proteínas Argonauta cargadas con pequeños RNAs viroidales y por otras ribonucleasas que generan RNAs subgenómicos

Minoia, Sofia (Universitat Politècnica de València, 2015-03-31)

Los viroides, los agentes infecciosos más simples de la escala biológica, están constituidos por una molécula circular de RNA monocatenario de aproximadamente 250-400 nucleótios (nt) que no codifica proteína alguna. A ...
Double-stranded RNA interferes in a sequence-specific manner with the infection of representative members of the two viroid families

CARBONELL, ALBERTO; Martínez de Alba, Ángel-Emilio; FLORES PEDAUYE, RICARDO; Gago, Selma (Elsevier, 2008-02-05)

[EN] Infection by viroids, non-protein-coding circular RNAs, occurs with the accumulation of 21¿24 nt viroid-derived small RNAs (vd-sRNAs) with characteristic properties of small interfering RNAs (siRNAs) associated to RNA ...
Estudio de las bases moleculares de la actividad supresora del silenciamiento por RNA de la proteína P0 codificada por virus de las venas amarillas de pimiento 5 (PeVYV-5).

Puértolas García, Victoria (Universitat Politècnica de València, 2022-03-30)

[ES] En plantas, una de las respuestas antivirales más eficientes está basada en el proceso de silenciamiento por RNA que es disparado por RNAs de doble cadena virales y que, en última instancia, conduce a la degradación ...
Fast-forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates

Carbonell, Alberto; López Del Rincón, Carmelo; Daròs, José-Antonio (Scientific Societies, 2019-02)

[EN] Artificial small RNAs (sRNAs), including artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are used to silence viral RNAs and confer antiviral resistance in plants. ...
Functional analysis of three Arabidopsis ARGONAUTES using slicer-defective mutants

CARBONELL, ALBERTO; Fahlgren, Noah; García-Ruíz, Hernan; Gilbert, Kerrigan B.; Montgomery, Taiowa A.; Nguyen, Tammy; Cuperus, Josh T.; Carrington, James C. (American Society of Plant Biologists, 2012-09)

[EN] In RNA-directed silencing pathways, ternary complexes result from small RNA-guided ARGONAUTE (AGO) associating with target transcripts. Target transcripts are often silenced through direct cleavage (slicing), ...
Heterologous RNA silencing suppressors from both plant- and animal-infecting viruses support Plum pox virus infection

Maliogka, Varvara I.; Calvo, Maria; CARBONELL, ALBERTO; Garcia, Juan Antonio; Valli, Adrian (Microbiology Society, 2012-07)

[EN] HCPro, the RNA silencing suppressor (RSS) of viruses belonging to the Potyvirus genus in the Potyviridae family, is a multifunctional protein presumably involved in all essential steps of the viral infection cycle. ...
Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors

CARBONELL, ALBERTO; Fahlgren, Noah; Mitchell, Skyler; Cox, Kevin L., Jr.; Reilly, Kevin C.; Mockler, Todd C.; Carrington, James C. (Blackwell Publishing, 2015-06)

[EN] Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective ...
How sequence variants of a plastid-replicating viroid with one single nucleotide change initiate disease in its natural host

Delgado Villar, Sonia Guadalupe; Navarro, Beatriz; Serra, Pedro; Gentit, Pascal; Cambra, Miguel Ángel; Chiumenti, Michela; De Stradis, Angelo; Di Serio, Francesco; FLORES PEDAUYE, RICARDO (Landes Bioscience, 2019-07-03)

[EN] Understanding how viruses and subviral agents initiate disease is central to plant pathology. Whether RNA silencing mediates the primary lesion triggered by viroids (small non-protein-coding RNAs), or just intermediate-late ...
Identification and Characterization of Stress-Responsive TAS3-Derived TasiRNAs in Melon

Cervera-Seco, Luis; Marques, M.C.; Sanz-Carbonell, Alejandro; Márquez-Molins, Joan; CARBONELL, ALBERTO; DAROS ARNAU, JOSE ANTONIO; Gomez, Gustavo Germán (Oxford University Press, 2019-11)

[EN] Small interfering RNAs (siRNA) are key regulators of gene expression that play essential roles in diverse biological processes. Trans-acting siRNAs (tasiRNAs) are a class of plant-endogenous siRNAs that lead the ...
In memoriam of Ricardo Flores: The career, achievements, and legacy of an inspirational plant virologist

Pallás Benet, Vicente; HERNANDEZ FORT, CARMEN; Marcos, Jose F.; Daròs, José-Antonio; Ambrós, Silvia; Navarro, Beatriz; NAVARRO BOHIGUES, JOSE ANTONIO; La Peña Del Rivero, Marcos De; Gago-Zachert, Selma; Gas, Maria E.; CARBONELL, ALBERTO; López Del Rincón, Carmelo; Martínez-De Alba, Angel E.; Di Serio, Francesco; Moreno, Pedro (Elsevier, 2022-04-15)

[EN] Ricardo Flores (1947-2020) focused his research on the identification, replication, pathogenesis, and evolution of viroids, the minimal non-protein-coding circular RNAs (250-400 nt) able to replicate and incite diseases ...
Inferring the Regulatory Network of the miRNA-mediated Response to Biotic and Abiotic Stress in Melon

Sanz-Carbonell, Alejandro; Marques Romero, Mª Carmen; Bustamante-González, Antonio Javier; Fares Riaño, Mario Ali; Rodrigo Tarrega, Guillermo; Gomez, Gustavo Germán (Springer (Biomed Central Ltd.), 2019-02-18)

[EN] Background: MiRNAs have emerged as key regulators of stress response in plants, suggesting their potential as candidates for knock-in/out to improve stress tolerance in agricultural crops. Although diverse assays have ...
Methods for Producing Transgenic Plants Resistant to CTV

Soler, Nuria; Plomer, Montserrat; Fagoaga, Carmen; Moreno, Pedro; Navarro, Luis; FLORES PEDAUYE, RICARDO; Peña, Leandro (Springer, 2019)

[EN] Conventional breeding of citrus types demands a long-term effort due to their complex reproductive biology and long juvenile period. As a compelling alternative, genetic engineering of mature tissues allows the insertion ...