CARBONELL, ALBERTO; Carrington, James C.(Elsevier, 2015-10)
[EN] ARGONAUTES (AGOs) are the effector proteins functioning in eukaryotic RNA silencing pathways. AGOs associate with small RNAs and are programmed to target complementary RNA or DNA. Plant viruses induce a potent and ...
[EN] Small RNAs (sRNAs) are loaded into ARGONAUTE (AGO) proteins to induce gene silencing. In plants, the 5¿-terminal nucleotide is important for sRNA sorting into different AGOs. Here we show that microRNA (miRNA) duplex ...
Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio(Wiley, 2016)
[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small
interfering RNAs (syn-tasiRNAs) are two classes of artificial small
RNAs (sRNAs) engineered to silence endogenous transcripts as
well as viral RNAs ...
Rodrigo Tarrega, Guillermo; Fares Riaño, Mario Ali(BioMed Central, 2012-04-13)
Background: The potential role of RNA molecules as gene expression regulators has led to a new perspective on the intracellular control and genome organization. Because secondary structures are crucial for their regulatory ...
Ausin, Israel; Feng, Suhua; Yu, Chaowei; Liu, Wanlu; Kuo, Hsuan Yu; Jacobsen, Elise L.; Zhai, Jixian; Gallego Bartolomé, Javier; Wang, Lin; Egertsdotter, Ulrika; Street, Nathaniel R.; Jacobsen, Steven E.; Wang, Haifeng(Proceedings of the National Academy of Sciences, 2016-12-13)
[EN] DNA methylation plays important roles in many biological processes, such as silencing of transposable elements, imprinting, and regulating gene expression. Many studies of DNA methylation have shown its essential roles ...
Rodrigo Tarrega, Guillermo; Majer, Eszter; Prakash, Satya; Daros Arnau, Jose Antonio; Jaramillo, Alfonso; Poyatos, Juan F.(Biophysical Society, 2015-09-01)
[EN] The regulation of gene expression, triggered by conformational changes in RNA molecules, is widely observed in cellular systems. Here, we examine this mode of control by means of a model-based design and construction ...
CARBONELL, ALBERTO; Carrington, James C.; Daros Arnau, Jose Antonio(Smart Science and Technology, 2016-01-12)
[EN] Artificial small RNAs (sRNAs) are short ≈21-nt non-coding RNAs engineered to inactivate sequence
complementary RNAs. In plants, they have been extensively used to silence cellular transcripts in gene function
analyses ...
ARGONAUTE (AGO) proteins function in small RNA (sRNA)-based RNA silencing pathways to regulate gene expression and control invading nucleic acids. In posttranscriptional RNA silencing pathways, plant AGOs associate with ...
Gilbert, Kerrigan B.; Fahlgren, Noah; Kasschau, Kristin D.; Chapman, Elisabeth J.; Carrington, James C.; CARBONELL, ALBERTO(Bio-Protocol, 2014-11-05)
[EN] High-throughput sequencing is a powerful tool for exploring small RNA populations in
plants. The ever-increasing output from an Illumina Sequencing System allows for multiplexing
multiple samples while still obtaining ...