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A circular RNA vector for targeted plant gene silencing based on an asymptomatic viroid

Marquez-Molins, Joan; Hernandez-Azurdia, Andrea Gabriela; Urrutia-Perez, María; Pallás Benet, Vicente; Gomez, Gustavo Germán (Blackwell Publishing, 2022-10)

[EN] Gene silencing for functional studies in plants has been largely facilitated by manipulating viral genomes with inserts from host genes to trigger virus-induced gene silencing (VIGS) against the corresponding mRNAs. ...
Highly specific gene silencing in a monocot species by artificial microRNAs derived from chimeric MIRNA precursors

CARBONELL, ALBERTO; Fahlgren, Noah; Mitchell, Skyler; Cox, Kevin L., Jr.; Reilly, Kevin C.; Mockler, Todd C.; Carrington, James C. (Blackwell Publishing, 2015-06)

[EN] Artificial microRNAs (amiRNAs) are used for selective gene silencing in plants. However, current methods to produce amiRNA constructs for silencing transcripts in monocot species are not suitable for simple, cost-effective ...
Ratiometric gibberellin biosensors for the analysis of signaling dynamics and metabolism in plant protoplasts

Andres, Jennifer; Schmunk, Lisa J.; Grau-Enguix, Federico; Braguy, Justine; Samodelov, Sophia L.; Blomeier, Tim; Ochoa-Fernandez, Rocio; Weber, Wilfried; AlBabili, Salim; Alabadí Diego, David; Blázquez, Miguel A.; Zurbriggen, Matias D. (Blackwell Publishing, 2024-05)

[EN] Gibberellins (GAs) are major regulators of developmental and growth processes in plants. Using the degradation-based signaling mechanism of GAs, we have built transcriptional regulator (DELLA)-based, genetically encoded ...

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