Viroids are single-stranded, circular, noncoding RNAs that infect plants, causing devastating diseases. In this work, we employed 2D DIGE, followed by MS identification, to analyze the response of tomato plants infected ...
Carbonell Olivares, Alberto; Daros Arnau, Jose Antonio(Wiley, 2016)
[EN] Artificial microRNAs (amiRNAs) and synthetic trans-acting small
interfering RNAs (syn-tasiRNAs) are two classes of artificial small
RNAs (sRNAs) engineered to silence endogenous transcripts as
well as viral RNAs ...
[EN] Artificial small RNAs (art-sRNAs), such as artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are highly specific 21-nucleotide small RNAs designed to recognize and silence ...
Yerga Castelló, Víctor(Universitat Politècnica de València, 2022-09-06)
[ES] Las ribozimas son biomoléculas enzimáticas cuyo centro catalítico está compuesto exclusivamente de ácido ribonucleico (RNA). Las ribozimas pequeñas de autocorte son una familia de ribozimas que se caracterizan por ...
[EN] Potato spindle tuber viroid (PSTVd) is a plant pathogen naturally infecting economically
important crops such as tomato (Solanum lycopersicum). Here, we aimed to engineer
tomato plants highly resistant to PSTVd and ...
López-Gresa, María Pilar; Payá, C.; Rodrigo Bravo, Ismael; Belles Albert, José Mª; Barceló-Cerdá, Susana; Hae Choi, Young; Verpoorte, Robert; Lisón, Purificación(MDPI AG, 2019-05-14)
[EN] Benzothiadiazole (BTH) is a functional analogue of the phytohormone salycilic acid (SA) involved in the plant immune response. NahG tomato plants are unable to accumulate SA, which makes them hypersusceptible to several ...
Eiras, Marcelo; Aragones, V.; Marqués, Jorge; Gómez-Jiménez, María Dolores; Daròs, José-Antonio(Springer (Biomed Central Ltd.), 2024-10-15)
[EN] Viroids that belong to genera Avsunviroid and Pelamovirod (family Avsunviroidae) replicate and accumulate in the chloroplasts of infected cells. In this report, we confirmed by RNA in situ hybridization using ...
[EN] Taxonomy
Eggplant latent viroid (ELVd) is the only species of the genus Elaviroid (family Avsunviroidae). All the viroids in the family Avsunviroidae contain hammerhead ribozymes in the strands of both polarities, ...
Castellano Pérez, Mayte(Universitat Politècnica de València, 2014-02-06)
[ES] EL objetivo del trabajo fue determinar el movimiento subcelular de los viroides ASBVd y PLMVd. Para
ello se crearon unas construcciones dónde la secuencia del viroide actuase como extremo 5'UTR del
gen de la GFP. ...
Márquez-Molins, Joan; NAVARRO BOHIGUES, JOSE ANTONIO; Pallás Benet, Vicente; Gomez, Gustavo Germán(Springer (Biomed Central Ltd.), 2019-08-01)
[EN] Background Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that ...
[EN] Members of the family Avsunviroidae have a single-stranded circular RNA genome that adopts a rod-like or branched conformation and can form, in the strands of either polarity, hammerhead ribozymes involved in their ...
Márquez Molins, Joan(Universitat Politècnica de València, 2022-06-20)
[ES] Los viroides son los patógenos con replicación autónoma más simples y sólo se han encontrado de forma natural infectando plantas superiores. Desde que se descubrieron en los años setenta, se ha adquirido un conocimiento ...
Silva Valencia, Samanta Gissela(Universitat Politècnica de València, 2023-02-13)
[ES] Los viroides son pequeños patógenos vegetales con capacidad autorreplicativa, constituidos por una cadena de RNA monocatenaria circular con una elevada estructura secundaria. Los viroides no tienen capacidad codificante; ...
Cervera, Amelia; Urbina, Denisse; La Peña del Rivero, Marcos de(BioMed Central, 2016-06-23)
[EN] Background: Catalytic RNAs, or ribozymes, are regarded as fossils of a prebiotic RNA world that have remained in
the genomes of modern organisms. The simplest ribozymes are the small self-cleaving RNAs, like the ...
[EN] Viroids are small, circular, highly structured pathogens that infect a broad range of plants, causing economic losses. Since their discovery in the 1970s, they have been considered as non-coding pathogens. In the last ...
[EN] Viroids are small (250-400 nucleotides), single-stranded, circular RNAs without protein-coding capacity that infect a large number of ornamental and crop plant species, causing high economic losses worldwide. Strategies ...
[EN] Viroids are tiny infectious agents of plants, consisting exclusively of a small (246–401 nucleotides)
circular RNA lacking protein-coding ability. Likely mimicking structural features of cellular RNAs,
they can ...
Carbonell, Alberto; Flores Pedauye, Ricardo; Gago Zachert, Selma Persida(Oxford University Press (OUP): Policy C - Option B, 2011)
[EN] Trans-cleaving hammerheads with discontinuous or extended stem I and with tertiary stabilizing motifs (TSMs) have been tested previously against short RNA substrates in vitro at low Mg2+ concentration. However, the ...
[EN] Despite being composed by a single-stranded, circular, non-protein-coding RNA of just 246-401 nucleotides (nt), viroids can incite in their host plants symptoms similar to those caused by DNA and RNA viruses, which ...
[EN] Viroids are small (246¿401 nucleotides), non-coding,
circular RNAs able to replicate autonomously in certain plants.
Viroids are classified into the families Pospiviroidae and
Avsunviroidae, whose members replicate ...