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Listar por palabra clave "Cas9"

Mostrando ítems 1-5 de 5

Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum)

Liu, Ying; Andersson, Mariette; GRANELL RICHART, ANTONIO; Cardi, Teodoro; Hofvander, Per; Nicolia, Alessandro (Springer-Verlag, 2022-09)

[EN] Key message We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with a high mutation ...
Estudio de diversos parámetros que afectan la edición genómica mediada por CRISPR-Cas12a/Cas9 en Nicotiana benthamiana

Sánchez Vicente, Javier (Universitat Politècnica de València, 2020-02-12)

[ES] En los últimos años, la tecnología CRISPR ha revolucionado la ingeniería del genoma en las plantas. El sistema CRISPR / Cas ofrece una alternativa para la generación de rupturas de doble cadena de ADN (DSB) en un lugar ...
PYL1-and PYL8-like ABA Receptors of Nicotiana benthamiana Play a Key Role in ABA Response in Seed and Vegetative Tissue

Pizzio, Gaston A.; Mayordomo, Cristian; Lozano-Juste, Jorge; García-Carpintero, Victor; Vazquez-Vilar, Marta; Nebauer, Sergio G.; Kaminski, Kacper P.; Ivanov, Nikolai V.; Estevez ,Juan C.; Rivera-Moreno, Maria; Albert, Armando; Orzáez Calatayud, Diego Vicente; Rodríguez, Pedro L. (MDPI, 2022-03)

[EN] To face the challenges of climate change and sustainable food production, it is essential to develop crop genome editing techniques to pinpoint key genes involved in abiotic stress signaling. The identification of ...
Simplifying plant gene silencing and genome editing logistics by a one-Agrobacterium system for simultaneous delivery of multipartite virus vectors

Aragones, V; Aliaga, F; Daròs, José-Antonio; Pasin, F (John Wiley & Sons, 2022-07)

[EN] Viral vectors provide a quick and effective way to express exogenous sequences in eukaryotic cells and to engineer eukaryotic genomes through the delivery of CRISPR/Cas components. Here, we presentJoinTRV, an improved ...
Strong and tunable anti-CRISPR/Cas activities in plants

Calvache-Román, Camilo Alberto; Selma, Sara; Vázquez-Vilar, Marta; Uranga, Mireia; Fernández-Del-Carmen, Asun; Daròs, José-Antonio; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-02)

[EN] CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This ...