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dc.contributor.author | Roque Mesa, Edelin Marta | es_ES |
dc.contributor.author | Fares Riaño, Mario Ali | es_ES |
dc.contributor.author | Yenush, Lynne | es_ES |
dc.contributor.author | Rochina Peñalver, Mª Cruz | es_ES |
dc.contributor.author | Wen, Jiangqi | es_ES |
dc.contributor.author | Mysore, Kirankumar S. | es_ES |
dc.contributor.author | Gómez Mena, María Concepción | es_ES |
dc.contributor.author | Beltran Porter, Jose Pio | es_ES |
dc.contributor.author | Cañas Clemente, Luís Antonio | es_ES |
dc.date.accessioned | 2018-04-21T04:20:25Z | |
dc.date.available | 2018-04-21T04:20:25Z | |
dc.date.issued | 2016 | es_ES |
dc.identifier.issn | 0022-0957 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/100803 | |
dc.description.abstract | [EN] PISTILLATA (PI) is a member of the B-function MADS-box gene family, which controls the identity of both petals and stamens in Arabidopsis thaliana. In Medicago truncatula (Mt), there are two PI-like paralogs, known as MtPI and MtNGL9. These genes differ in their expression patterns, but it is not known whether their functions have also diverged. Describing the evolution of certain duplicated genes, such as transcription factors, remains a challenge owing to the complex expression patterns and functional divergence between the gene copies. Here, we report a number of functional studies, including analyses of gene expression, protein-protein interactions, and reverse genetic approaches designed to demonstrate the respective contributions of each M. truncatula PI-like paralog to the B-function in this species. Also, we have integrated molecular evolution approaches to determine the mode of evolution of Mt PI-like genes after duplication. Our results demonstrate that MtPI functions as a master regulator of B-function in M. truncatula, maintaining the overall ancestral function, while MtNGL9 does not seem to have a role in this regard, suggesting that the pseudogenization could be the functional evolutionary fate for this gene. However, we provide evidence that purifying selection is the primary evolutionary force acting on this paralog, pinpointing the conservation of its biochemical function and, alternatively, the acquisition of a new role for this gene. | es_ES |
dc.description.sponsorship | This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (MINECO; BIO2009-08134 and BIO2012-39849-CO2-01). MAF was supported by a grant from the MINECO (BFU2012-36346). We wish to thank Drs Santiago F. Elena Fito (IBMCP, CSIC-UPV), Jose V. Gimeno Alcaniz (IATA, CSIC), Javier Paz-Ares (CNB, CSIC), and Carlos Alonso-Blanco (CNB, CSIC) for valuable suggestions and comments in the initial stages of this work. The technical assistance of Rafael Martinez-Pardo in the greenhouse is gratefully acknowledged. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Oxford University Press | es_ES |
dc.relation.ispartof | Journal of Experimental Botany | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Duplicated B-function genes | es_ES |
dc.subject | Functional analyses | es_ES |
dc.subject | MADS-box transcription factors | es_ES |
dc.subject | Medicago truncatula | es_ES |
dc.subject | Molecular evolution | es_ES |
dc.subject | PISTILLATA-like | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Evolution by gene duplication of Medicago truncatula PISTILLATA-like transcription factors | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1093/jxb/erv571 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BFU2012-36346/ES/EL PAPEL DE LA DUPLICACION GENICA EN LA COMPLEJIDAD DE SISTEMAS BIOLOGICOS: RE-DIRECCION DE DINAMICAS MUTACIONALES Y ORIGEN DE INNOVACIONES BIOLOGICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//BIO2009-08134/ES/Mejora Del Valor Nutritivo De La Alfalfa (Medicago Sativa L.) Mediante Ingenieria Genetica/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BIO2012-39849-C02-01/ES/AISLAMIENTO Y CARACTERIZACION DE GENES QUE CONTROLAN CARACTERES DE INTERES AGRONOMICO EN LA ALFALFA (MEDICAGO SATIVA L.). DESARROLLO DE HERRAMIENTAS BIOTECNOLOGICAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Roque Mesa, EM.; Fares Riaño, MA.; Yenush, L.; Rochina Peñalver, MC.; Wen, J.; Mysore, KS.; Gómez Mena, MC.... (2016). Evolution by gene duplication of Medicago truncatula PISTILLATA-like transcription factors. Journal of Experimental Botany. 67(6):1805-1817. https://doi.org/10.1093/jxb/erv571 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1093/jxb/erv571 | es_ES |
dc.description.upvformatpinicio | 1805 | es_ES |
dc.description.upvformatpfin | 1817 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 67 | es_ES |
dc.description.issue | 6 | es_ES |
dc.identifier.pmid | 26773809 | |
dc.identifier.pmcid | PMC4783364 | |
dc.relation.pasarela | S\318948 | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |