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Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits

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Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits

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dc.contributor.author Abad-Guamán, Rodrigo es_ES
dc.contributor.author Larrea-Dávalos, Jose Antonio es_ES
dc.contributor.author Carabaño, Rosa es_ES
dc.contributor.author García, Javier es_ES
dc.contributor.author Carro, Maria Dolores es_ES
dc.date.accessioned 2019-01-03T12:32:17Z
dc.date.available 2019-01-03T12:32:17Z
dc.date.issued 2018-09-28
dc.identifier.issn 1257-5011
dc.identifier.uri http://hdl.handle.net/10251/114685
dc.description.abstract [EN] Two in vitro experiments were performed to analyse the fermentative potential of ileal content, caecal content, soft faeces and hard faeces from adult rabbits. Experiment 1 evaluated 3 doses (0.5, 1.0 and 2.0 g fresh digesta/g substrate dry matter [DM]) of ileal and caecal digesta as inoculum in 28 h-incubations. Two ileal and 2 caecal inocula were obtained, each by pooling the ileal or caecal digesta of 2 adult rabbits. Pectin from sugar beet pulp (SBP) and the insoluble residue obtained after a 2-step in vitro pre-digestion of SBP and wheat straw were used as substrates. The 0.5 dose produced the lowest (P<0.05) amount of gas at 28 h, with no differences (P>0.05) between the 1.0 and 2.0 doses (44.9, 51.6 and 53.8 mL/g substrate DM, respectively; values averaged across inocula and substrates). Experiment 2 evaluated two doses of ileal inoculum (1 and 1.5 g fresh digesta/g substrate DM) and compared ileal digesta, caecal digesta, soft faeces and hard faeces as inoculum for determining in vitro gas production (144-h incubations) of the 3 substrates used in Experiment 1 and wheat starch. Three inocula of each type were obtained, each by pooling either digesta or faeces from 3 rabbits. There were no differences (P>0.05) between the 2 ileal doses tested in gas production parameters, and therefore the 1.0 dose was selected for further ileal fermentations. Starch and pectin showed similar (P>0.05) values of gas production rate and maximal gas production rate when they were fermented with caecal digesta (0.038 vs. 0.043%/h, and 13.7 vs. 15.2 mL/h, respectively), soft (0.022 vs. 0.031%/h, and 9.97 vs. 9.33 mL/h) and hard faeces (0.031 vs. 0.038%/h, and 13.6 vs. 10.8 mL/h), and values were higher than those for SBP and wheat straw; in contrast, values for starch and pectin differed with the ileal inoculum (0.046 vs. 0.024%/h, and 18.4 vs. 6.60 mL/h). Both ileal and caecal gas production parameters were well correlated with those for hard and soft faeces inocula, respectively (r≥0.77; P≤0.040). The ileal inoculum showed a relevant fermentative potential, but lower than that of caecal digesta and soft and hard faeces for all substrates except wheat starch. es_ES
dc.description.sponsorship Funding from the Spanish Ministry of Economy and Competitiveness (Project AGL2011-22628) and the Comunidad Autónoma de Madrid (CAM; Project MEDGAN ABI-2913) is gratefully acknowledged. es_ES
dc.language Inglés es_ES
dc.publisher Universitat Politècnica de València
dc.relation.ispartof World Rabbit Science
dc.rights Reserva de todos los derechos es_ES
dc.subject In vitro es_ES
dc.subject Gas production es_ES
dc.subject Sugar beet pulp es_ES
dc.subject Pectin es_ES
dc.subject Wheat straw es_ES
dc.subject Starch es_ES
dc.subject Rabbit es_ES
dc.title Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits es_ES
dc.type Artículo es_ES
dc.date.updated 2019-01-03T11:41:54Z
dc.identifier.doi 10.4995/wrs.2018.9726
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2011-22628/ES/ESTUDIO DE LA MICROBIOTA EN SISTEMAS IN VITRO DE SIMULACION DE LA FERMENTACION RUMINAL (CULTIVOS NO RENOVADOS, FERMENTADORES RUSITEC Y FERMENTADORES DE FLUJO CONTINUO)/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/CAM//S2013%2FABI-2913/ es_ES
dc.rights.accessRights Abierto es_ES
dc.description.bibliographicCitation Abad-Guamán, R.; Larrea-Dávalos, JA.; Carabaño, R.; García, J.; Carro, MD. (2018). Influence of inoculum type (ileal, caecal and faecal) on the in vitro fermentation of different sources of carbohydrates in rabbits. World Rabbit Science. 26(3):227-240. https://doi.org/10.4995/wrs.2018.9726 es_ES
dc.description.accrualMethod SWORD es_ES
dc.relation.publisherversion https://doi.org/10.4995/wrs.2018.9726 es_ES
dc.description.upvformatpinicio 227 es_ES
dc.description.upvformatpfin 240 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 26
dc.description.issue 3
dc.identifier.eissn 1989-8886
dc.contributor.funder Comunidad de Madrid es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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