Mostrar el registro sencillo del ítem
dc.contributor.author | Li, Hanqing | es_ES |
dc.contributor.author | Watson, Ash | es_ES |
dc.contributor.author | Olechwier, Agnieszka | es_ES |
dc.contributor.author | Anaya, Michael | es_ES |
dc.contributor.author | Sorooshyari, Siamak K. | es_ES |
dc.contributor.author | Harnett, Dermott P. | es_ES |
dc.contributor.author | Lee, Hyung-Kook (Peter) | es_ES |
dc.contributor.author | Vielmetter, Jost | es_ES |
dc.contributor.author | Fares Riaño, Mario Ali | es_ES |
dc.contributor.author | Garcia, K. Christopher | es_ES |
dc.contributor.author | Ozkan, Engin | es_ES |
dc.contributor.author | Labrador, Juan-Pablo | es_ES |
dc.contributor.author | Zinn, Kai | |
dc.date.accessioned | 2019-01-16T21:03:49Z | |
dc.date.available | 2019-01-16T21:03:49Z | |
dc.date.issued | 2017 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/115606 | |
dc.description.abstract | [EN] An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons. | es_ES |
dc.description.sponsorship | This work was supported by NIH grants to KZ (R37 NS28182), and to EO¨ (RO1 NS097161), by the Klingenstein-Simons Fellowship in the Neurosciences to EO¨ , and by SFI grants 07/IN.1/B913 and 08/ RFP/NSC1617 to J-P L. We thank Aref Arzan Zarin for preliminary genetic analysis. We thank Elena Armand and Suzanne Fisher for technical assistance, Maria Prats for preparation of AP supernatants for the experiments of Figs. S2 and S3, and Violana Nesterova for figure preparation. We thank Kaushiki Menon and Namrata Bali for help with larval staining. We acknowledge Dr. Elena Solomaha and the University of Chicago BioPhysics Core Facilities for training with and access to a Biacore 3000. We thank Laura Quintana Rio, Lalanti Venkatasubramanian, and Richard Mann (Columbia) for the Side-VI-T2A-GAL4 line. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | eLife Sciences Publications | es_ES |
dc.relation.ispartof | eLife | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.title | Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.7554/eLife.28111 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/J4NIH/NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE/5R01NS097161-02/US/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/J4SFI/SFI Research Frontiers Programme (RFP)/08/RFP/NSC1617/IE/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/J4SFI/SFI Principal Investigator Programme (PI)/07/IN.1/B913/IE/ | |
dc.relation.projectID | info:eu-repo/grantAgreement/J4NIH/NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE/5R37NS028182-26/US/ | |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Li, H.; Watson, A.; Olechwier, A.; Anaya, M.; Sorooshyari, SK.; Harnett, DP.; Lee, H(.... (2017). Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development. eLife. 6:1-24. doi:10.7554/eLife.28111 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.7554/eLife.28111.001 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 24 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 6 | es_ES |
dc.identifier.eissn | 2050-084X | es_ES |
dc.identifier.pmid | 28829740 | |
dc.identifier.pmcid | PMC5578738 | |
dc.relation.pasarela | S\355995 | es_ES |
dc.contributor.funder | National Institutes of Health, EEUU | |
dc.contributor.funder | Esther A. and Joseph Klingenstein Fund |