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dc.contributor.author | Tortajada-Genaro, Luis Antonio | es_ES |
dc.contributor.author | Yamanaka, Eric Seiti | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.date.accessioned | 2020-04-24T07:14:03Z | |
dc.date.available | 2020-04-24T07:14:03Z | |
dc.date.issued | 2019 | es_ES |
dc.identifier.issn | 0039-9140 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/141451 | |
dc.description.abstract | [EN] Consumer electronic technologies offer practical performances to develop compact biosensing systems intended for the point-of-care testing of DNA biomarkers. Herein a discrimination method for detecting single nucleotide polymorphisms, based on isothermal amplification and on-chip hybridisation, was developed and integrated into user-friendly optical devices: e.g., USB digital microscope, flatbed scanner, smartphone and DVD drive. In order to adequately identify a single base change, loop-mediated isothermal amplification (LAMP) was employed, with high yields (8 orders) within 45 min. Subsequently, products were directly hybridised to the allele-specific probes attached to plastic chips in an array format. After colorimetric staining, four consumer electronic techniques were compared. Sensitive precise measurements were taken (high signal-to-noise ratios, 10-mu m image resolution, 99% scan-to-scan reproducibility). These features confirmed their potential as analytical tools, are a competitive alternative to fluorescence scanners, and incorporate additional advantages, such as user-friendly interface and connectivity for telemedicine needs. The analytical performances of the integrated platform (assay and reader) in the human samples were also excellent, with a low detection limit (100 genomic DNA copies), and reproducible (< 15%) and cheap assays (< 10 (sic)/test). The correct genotyping of a genetic biomarker (single-nucleotide polymorphism located in the GRIK4 gene) was achieved as the assigned genotypes agreed with those determined by using sequencing. The portability, favourable discriminating and read-out capabilities reveal that the implementation of mass-produced low-cost devices into minimal-specialised clinical laboratories is closer to becoming a reality. | es_ES |
dc.description.sponsorship | The authors acknowledge the financial support received from the Generalitat Valenciana, Spain (GVA-PROMETEOII/2014/040 Project and GRISOLIA/2014/024 Ph.D. grant) and the Spanish Ministry of Economy and Competitiveness, Spain (MINECO CTQ2016-75749-R project) by U. E. FEDER funds. The authors also thank J. Carrascosa for supporting the DVD reader measurements. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Talanta | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Single-nucleotide polymorphism | es_ES |
dc.subject | Isothermal DNA amplification | es_ES |
dc.subject | Point-of-care testing | es_ES |
dc.subject | Smartphone | es_ES |
dc.subject | Scanner | es_ES |
dc.subject | Compact disc | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.talanta.2019.01.124 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GRISOLIA%2F2014%2F024/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2016-75749-R/ES/BIOSENSORES HOLOGRAFICOS. PRUEBA DE CONCEPTO Y DEMOSTRACION EN APLICACIONES CLINICAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Tortajada-Genaro, LA.; Yamanaka, ES.; Maquieira Catala, A. (2019). Consumer electronics devices for DNA genotyping based on loop-mediated isothermal amplification and array hybridisation. Talanta. 198:424-431. https://doi.org/10.1016/j.talanta.2019.01.124 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1016/j.talanta.2019.01.124 | es_ES |
dc.description.upvformatpinicio | 424 | es_ES |
dc.description.upvformatpfin | 431 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 198 | es_ES |
dc.relation.pasarela | S\388821 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |