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Transcriptional analysis of intramuscular fatty acid composition in the longissimus thoracis muscle of Iberian × Landrace back-crossed pigs

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Transcriptional analysis of intramuscular fatty acid composition in the longissimus thoracis muscle of Iberian × Landrace back-crossed pigs

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dc.contributor.author Pena, R. es_ES
dc.contributor.author Noguera, J.L. es_ES
dc.contributor.author Casellas, J. es_ES
dc.contributor.author Diaz, I. es_ES
dc.contributor.author Díaz Fernández, Ana Isabel es_ES
dc.contributor.author Folch, J.M. es_ES
dc.contributor.author Ibañez Escriche, Noelia es_ES
dc.date.accessioned 2020-10-07T03:35:10Z
dc.date.available 2020-10-07T03:35:10Z
dc.date.issued 2013-12 es_ES
dc.identifier.issn 0268-9146 es_ES
dc.identifier.uri http://hdl.handle.net/10251/151310
dc.description.abstract [EN] This study aimed at identifying differential gene expression conditional on the fatty acid profile of the longissimus thoracis (Lt) muscle, a prime cut of economic relevance for fresh and cured pork production. A population of 110 Iberian (25%)xLandrace (75%) back-crossed pigs was used, because these two breeds exhibit extreme profiles of intramuscular saturated fatty acid, monounsaturated fatty acid (MUFA) and polyunsaturated fatty acid (PUFA) contents. Total RNA from Lt muscle was individually hybridized to GeneChip Porcine Genome arrays (Affymetrix). A principal component analysis was performed with data from the 110 animals to select 40 extreme animals based on the total fatty acid profile and the MUFA composition (MAP). Comparison of global transcription levels between extreme fatty acid profile pigs (n=40) resulted in 219 differentially expressed probes (false discovery rate <0.10). Gene ontology, pathway and network analysis indicated that animals with higher percentages of PUFA exhibit a shift toward a more oxidative muscular metabolism state, with a raise in mitochondria function (PPARGC1A, ATF2), fatty acid uptake and oxidation (FABP5, MGLL). On the other hand, 87 probes were differentially expressed between MUFA composition groups (n=40; false discovery rate <0.10). In particular, muscles rich in n-7 MUFA expressed higher levels of genes involved in lipid metabolism (GLUL, CRAT, PLA2G15) and lower levels of fatty acid elongation genes (ELOVL5). Moreover, the chromosomal position of FABP5, PAQR3, MGLL, PPARGC1A, GLUL and ELOVL5 co-localized with very relevant QTL for fat deposition and composition described in the same resource population. This study represents a complementary approach to identifying genes underlying these QTL effects. es_ES
dc.description.sponsorship This work was funded by MICINN project AGL2008-04818-C03/GAN. The authors gratefully acknowledge NOVA GENETICA S.A. and the cooperative COPAGA for their collaboration and would particularly like to thank Eva Ramells, Pere Borras, Josep M Borras and Felix Grau. es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation.ispartof Animal Genetics es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Fatty acid profile es_ES
dc.subject Gene expression es_ES
dc.subject Microarray es_ES
dc.subject Muscle es_ES
dc.subject Porcine es_ES
dc.subject.classification PRODUCCION ANIMAL es_ES
dc.title Transcriptional analysis of intramuscular fatty acid composition in the longissimus thoracis muscle of Iberian × Landrace back-crossed pigs es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/age.12066 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04818-C03-03/ES/GENES CANDIDATOS E IDENTIFICACION GENOMICA DE LOCI Y RUTAS GENICAS QUE AFECTAN A LA CALIDAD DE LA CARNE EN CERDOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04818-C03-02/ES/GENES CANDIDATOS E IDENTIFICACION GENOMICA DE LOCI Y RUTAS GENETICAS QUE AFECTAN A LA CALIDAD DE LA CARNE EN CERDOS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MICINN//AGL2008-04818-C03-01/ES/GENES CANDIDATOS E IDENTIFICACION GENOMICA DE LOCI Y RUTAS GENETICAS QUE AFECTAN A LA CALIDAD DE LA CARNE EN CERDOS/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Ciencia Animal - Departament de Ciència Animal es_ES
dc.description.bibliographicCitation Pena, R.; Noguera, J.; Casellas, J.; Diaz, I.; Díaz Fernández, AI.; Folch, J.; Ibañez Escriche, N. (2013). Transcriptional analysis of intramuscular fatty acid composition in the longissimus thoracis muscle of Iberian × Landrace back-crossed pigs. Animal Genetics. 44(6):648-660. https://doi.org/10.1111/age.12066 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/age.12066 es_ES
dc.description.upvformatpinicio 648 es_ES
dc.description.upvformatpfin 660 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 44 es_ES
dc.description.issue 6 es_ES
dc.identifier.pmid 23826865 es_ES
dc.relation.pasarela S\343538 es_ES
dc.contributor.funder Ministerio de Ciencia e Innovación es_ES
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