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Fast-forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates

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Fast-forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates

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dc.contributor.author Carbonell, Alberto es_ES
dc.contributor.author López Del Rincón, Carmelo es_ES
dc.contributor.author Daròs, José-Antonio es_ES
dc.date.accessioned 2021-01-26T04:32:13Z
dc.date.available 2021-01-26T04:32:13Z
dc.date.issued 2019-02 es_ES
dc.identifier.issn 0894-0282 es_ES
dc.identifier.uri http://hdl.handle.net/10251/159845
dc.description.abstract [EN] Artificial small RNAs (sRNAs), including artificial microRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs), are used to silence viral RNAs and confer antiviral resistance in plants. Here, the combined use of recent high-throughput methods for generating artificial sRNA constructs and the Tomato spotted wilt virus (TSWV)¿Nicotiana benthamiana pathosystem allowed for the simple and rapid identification of amiRNAs with high anti-TSWV activity. A comparative analysis between the most effective amiRNA construct and a syn-tasiRNA construct including the four most effective amiRNA sequences showed that both were highly effective against two different TSWV isolates. These results highlight the usefulness of this high-throughput methodology for the fast-forward identification of artificial sRNAs with high antiviral activity prior to timeconsuming generation of stably transformed plants. es_ES
dc.description.sponsorship This study was supported by Ministerio de Economia y Competitividad (Spain) grant BIO2014-54269-R (cofounded FEDER funds) and Ministerio de Ciencia, Innovacion y Universidades (Spain) grant BIO2017-83184-R (cofounded FEDER funds) to J.-A. Daros, and by Instituto Nacional de Investigaciones Agrarias grant RTA2013-00047-C02-02 (cofounded FEDER funds) grant RTA2013-00047-C02-02 to C. Lopez. A. Carbonell was the recipient of an Individual Fellowship from the European Union Horizon 2020 research and innovation programme under the Marie Sklodowska Curie Individual Fellowship, European Union Horizon 2020 grant number 655841, and was selected in the Ramon y Cajal programme (RYC-2017-21648) from Ministerio de Ciencia, Innovacion y Universidades (Spain). es_ES
dc.language Inglés es_ES
dc.publisher Scientific Societies es_ES
dc.relation.ispartof Molecular Plant-Microbe Interactions es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject RNA silencing es_ES
dc.subject Artificial small RNAs es_ES
dc.subject Artificial microRNAs es_ES
dc.subject Synthetic trans-acting siRNAs es_ES
dc.subject Antiviral resistance es_ES
dc.subject TSWV es_ES
dc.subject.classification GENETICA es_ES
dc.title Fast-forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1094/MPMI-05-18-0117-TA es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2014-54269-R/ES/INSTRUMENTOS BIOTECNOLOGICOS DERIVADOS DE VIRUS DE PLANTAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/655841/EU/Genome-wide analysis of RNA and protein interacting profiles during a plant virus infection/
dc.relation.projectID info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2017-83184-R/ES/VIRUS DE PLANTAS: PATOGENOS Y TAMBIEN VECTORES PARA LA PRODUCCION DE PROTEINAS, METABOLITOS, RNAS Y NANOPARTICULAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//RYC-2017-21648/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//RTA2013-00047-C02-02/ES/Búsqueda de nuevas fuentes de resistencia y desarrollo de microRNAs artificiales para el control del virus del bronceado del tomate (TSWV)/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Carbonell, A.; López Del Rincón, C.; Daròs, J. (2019). Fast-forward Identification of Highly Effective Artificial Small RNAs Against Different Tomato spotted wilt virus Isolates. Molecular Plant-Microbe Interactions. 32(2):142-156. https://doi.org/10.1094/MPMI-05-18-0117-TA es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1094/MPMI-05-18-0117-TA es_ES
dc.description.upvformatpinicio 142 es_ES
dc.description.upvformatpfin 156 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 32 es_ES
dc.description.issue 2 es_ES
dc.identifier.pmid 30070616 es_ES
dc.relation.pasarela S\377282 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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