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Engineered 3D hydrogels with full-length fibronectin that sequester and present growth factors

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Engineered 3D hydrogels with full-length fibronectin that sequester and present growth factors

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dc.contributor.author Trujillo-Muñoz, Sara es_ES
dc.contributor.author González-García, Cristina es_ES
dc.contributor.author Rico Tortosa, Patricia María es_ES
dc.contributor.author Reid, Andrew es_ES
dc.contributor.author Windmill, James es_ES
dc.contributor.author Dalby, Matthew J. es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2021-05-05T03:31:50Z
dc.date.available 2021-05-05T03:31:50Z
dc.date.issued 2020-09 es_ES
dc.identifier.issn 0142-9612 es_ES
dc.identifier.uri http://hdl.handle.net/10251/165955
dc.description.abstract [EN] Extracellular matrix (ECM)-derived matrices such as Matrigel are used to culture numerous cell types in vitro as they recapitulate ECM properties that support cell growth, organisation, migration and differentiation. These ECM-derived matrices contain various growth factors which make them highly bioactive. However, they suffer lot-to-lot variability, undefined composition and lack of controlled physical properties. There is a need to develop rationally designed biomaterials that can also recapitulate ECM roles. Here, we report the development of fibronectin (FN)-based 3D hydrogels of controlled stiffness and degradability that incorporate full-length FN to enable solid-phase presentation of growth factors in a physiological manner. We demonstrate, in vitro and in vivo, the effect of incorporating vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP2) in these hydrogels to enhance angiogenesis and bone regeneration, respectively. These hydrogels represent a step-change in the design of well-defined, reproducible, synthetic microenvironments for 3D cell culture that incorporate growth factors to achieve functional effects. es_ES
dc.description.sponsorship This study was supported by the UK Regenerative Medicine Platform (MRC grant MR/L022710/1), the UK Engineering and Physical Sciences Research Council (EPSRC EP/P001114/1) and a programme grant from the Sir Bobby Charlton Foundation. mu CT work was supported by the European Research Council (ERC) under the European Union's Seventh Framework Programme (FP7/2007-2013) (grant agreement No. [615030]). S.T. acknowledges support from the University of Glasgow through their internal scholarship funding program. We thank the support of David Adams (Anatomy lab, University of Glasgow) and the Biological Services and Veterinary Research Facility (University of Glasagow) for their assistance. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Biomaterials es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Hydrogels es_ES
dc.subject Growth factors es_ES
dc.subject Fibronectin es_ES
dc.subject Poly(ethylene) glycol es_ES
dc.subject Bone es_ES
dc.subject Vascularisation es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.title Engineered 3D hydrogels with full-length fibronectin that sequester and present growth factors es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.biomaterials.2020.120104 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/615030/EU/Soft and Small: Acoustic Transducers Inspired by Nature/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//EP%2FP001114%2F1/GB/Engineering growth factor microenvironments - a new therapeutic paradigm for regenerative medicine/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/UKRI//MR%2FL022710%2F1/GB/Synergistic microenvironments for non-union bone defects/
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Centro de Biomateriales e Ingeniería Tisular - Centre de Biomaterials i Enginyeria Tissular es_ES
dc.description.bibliographicCitation Trujillo-Muñoz, S.; González-García, C.; Rico Tortosa, PM.; Reid, A.; Windmill, J.; Dalby, MJ.; Salmerón Sánchez, M. (2020). Engineered 3D hydrogels with full-length fibronectin that sequester and present growth factors. Biomaterials. 252:1-15. https://doi.org/10.1016/j.biomaterials.2020.120104 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.biomaterials.2020.120104 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 15 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 252 es_ES
dc.identifier.pmid 32422492 es_ES
dc.relation.pasarela S\434898 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder UK Research and Innovation es_ES
dc.contributor.funder University of Glasgow es_ES
dc.contributor.funder Sir Bobby Charlton Foundation es_ES
dc.contributor.funder UK Regenerative Medicine Platform es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido es_ES
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