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Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid - Gelatin injectable hydrogels

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Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid - Gelatin injectable hydrogels

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dc.contributor.author Vaca-González, Juan Jairo es_ES
dc.contributor.author Clara-Trujillo, Sandra es_ES
dc.contributor.author Guillot-Ferriols, María Teresa es_ES
dc.contributor.author Ródenas Rochina, Joaquín es_ES
dc.contributor.author Sanchis Sánchez, María Jesús es_ES
dc.contributor.author Gómez Ribelles, José Luís es_ES
dc.contributor.author Garzón-Alvarado, Diego Alexander es_ES
dc.contributor.author Gallego Ferrer, Gloria es_ES
dc.date.accessioned 2021-05-12T03:32:23Z
dc.date.available 2021-05-12T03:32:23Z
dc.date.issued 2020-08 es_ES
dc.identifier.issn 1567-5394 es_ES
dc.identifier.uri http://hdl.handle.net/10251/166218
dc.description.abstract [EN] Electrical stimulation (ES) has provided enhanced chondrogenesis of mesenchymal stem cells (MSCs) cultured in micro-mass without the addition of exogenous growth factors. In this study, we demonstrate for the first time that ES of MSCs encapsulated in an injectable hyaluronic acid (HA) - gelatin (GEL) mixture enhances the chondrogenic potential of the hydrogel. Samples were stimulated for 21 days with 10 mV/cm at 60 kHz, applied for 30 min every 6 h a day. Mechanical properties of hydrogels were higher if the precursors were dissolved in Calcium-Free Krebs Ringer Buffer (G' = 1141 +/- 23 Pa) compared to those diluted in culture media (G' = 213 +/- 19 Pa). Cells within stimulated hydrogels were rounder (55%) than non-stimulated cultures (32%) (p = 0.005). Chondrogenic markers such as SOX-9 and aggrecan were higher in stimulated hydrogels compared to controls. The ES demonstrated that normalized content of glycosaminoglycans and collagen to DNA was slightly higher in stimulated samples. Additionally, collagen type II normalized to total collagen was 2.43 times higher in stimulated hydrogels. These findings make ES a promising tool for enhancing articular cartilage tissue engineering outcomes by combining hydrogels and MSCs. es_ES
dc.description.sponsorship The financial support received from COLCIENCIAS through Fellowship No. 647 and Grant 712-2015 No. 50457 is acknowledged, as is that from the Spain Ministry of Economy and Competitiveness through the MAT2016-76039-C4-1-R project (including the Feder Funds). es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Bioelectrochemistry es_ES
dc.rights Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) es_ES
dc.subject Chondrogenic differentiation es_ES
dc.subject Electric fields es_ES
dc.subject Injectable hydrogels es_ES
dc.subject Hyaluronic acid es_ES
dc.subject Gelatin Mesenchymal stem cells es_ES
dc.subject.classification INGENIERIA QUIMICA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.title Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid - Gelatin injectable hydrogels es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.bioelechem.2020.107536 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COLCIENCIAS//712-2015 50457/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/COLCIENCIAS//647/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//MAT2016-76039-C4-1-R/ES/BIOMATERIALES PIEZOELECTRICOS PARA LA DIFERENCIACION CELULAR EN INTERFASES CELULA-MATERIAL ELECTRICAMENTE ACTIVAS/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Vaca-González, JJ.; Clara-Trujillo, S.; Guillot-Ferriols, MT.; Ródenas Rochina, J.; Sanchis Sánchez, MJ.; Gómez Ribelles, JL.; Garzón-Alvarado, DA.... (2020). Effect of electrical stimulation on chondrogenic differentiation of mesenchymal stem cells cultured in hyaluronic acid - Gelatin injectable hydrogels. Bioelectrochemistry. 134:1-11. https://doi.org/10.1016/j.bioelechem.2020.107536 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.bioelechem.2020.107536 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 11 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 134 es_ES
dc.identifier.pmid 32335352 es_ES
dc.relation.pasarela S\409236 es_ES
dc.contributor.funder Agencia Estatal de Investigación es_ES
dc.contributor.funder European Regional Development Fund es_ES
dc.contributor.funder Departamento Administrativo de Ciencia, Tecnología e Innovación, Colombia es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
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