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dc.contributor.author | Oliveira Leastro, Mikhail | es_ES |
dc.contributor.author | Villar-Álvarez, David | es_ES |
dc.contributor.author | Freitas-Astúa, Juliana | es_ES |
dc.contributor.author | Watanabe Kitajima, Elliot | es_ES |
dc.contributor.author | Pallás Benet, Vicente | es_ES |
dc.contributor.author | SANCHEZ NAVARRO, JESUS ANGEL | es_ES |
dc.date.accessioned | 2022-05-25T18:03:56Z | |
dc.date.available | 2022-05-25T18:03:56Z | |
dc.date.issued | 2021-12 | es_ES |
dc.identifier.issn | 1999-4915 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/182912 | |
dc.description.abstract | [EN] Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. | es_ES |
dc.description.sponsorship | This research was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), grant numbers 2014/0845-9, 2017/50222-0, 2015/10249-1, 2017/19898-8 and by the Spanish Agencia Estatal de Investigación (AEI) and Fondo Europeo de Desarrollo Regional (FEDER), grant number PID2020-115571RB-100. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Viruses | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Viral fitness | es_ES |
dc.subject | Cilevirus | es_ES |
dc.subject | Alfalfa mosaic virus system | es_ES |
dc.subject | Movement protein | es_ES |
dc.subject | Amino acid mutation | es_ES |
dc.title | Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/v13122498 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2014%2F08458-9/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2017%2F50222-0/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2015%2F10249-1/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/FAPESP//2017%2F19898-8/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.description.bibliographicCitation | Oliveira Leastro, M.; Villar-Álvarez, D.; Freitas-Astúa, J.; Watanabe Kitajima, E.; Pallás Benet, V.; Sanchez Navarro, JA. (2021). Spontaneous Mutation in the Movement Protein of Citrus Leprosis Virus C2, in a Heterologous Virus Infection Context, Increases Cell-to-Cell Transport and Generates Fitness Advantage. Viruses. 13(12):1-16. https://doi.org/10.3390/v13122498 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/v13122498 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 16 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 13 | es_ES |
dc.description.issue | 12 | es_ES |
dc.identifier.pmid | 34960766 | es_ES |
dc.identifier.pmcid | PMC8708801 | es_ES |
dc.relation.pasarela | S\460373 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Fundação de Amparo à Pesquisa do Estado de São Paulo | es_ES |