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dc.contributor.author | Martínez-Pérez, Mireya | es_ES |
dc.contributor.author | Gómez Mena, María Concepción | es_ES |
dc.contributor.author | Alvarado-Marchena, Luis | es_ES |
dc.contributor.author | Nadi, Riad | es_ES |
dc.contributor.author | Micol, José Luis | es_ES |
dc.contributor.author | Pallás Benet, Vicente | es_ES |
dc.contributor.author | APARICIO HERRERO, FREDERIC | es_ES |
dc.date.accessioned | 2022-06-16T18:05:55Z | |
dc.date.available | 2022-06-16T18:05:55Z | |
dc.date.issued | 2021-10-04 | es_ES |
dc.identifier.issn | 1664-302X | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/183413 | |
dc.description.abstract | [EN] The N-6-methyladenosine (m(6)A) pathway has been widely described as a viral regulatory mechanism in animals. We previously reported that the capsid protein (CP) of alfalfa mosaic virus (AMV) interacts with the Arabidopsis m(6)A demethylase ALKBH9B regulating m(6)A abundance on viral RNAs (vRNAs) and systemic invasion of floral stems. Here, we analyze the involvement of other ALKBH9 proteins in AMV infection and we carry out a detailed evaluation of the infection restraint observed in alkbh9b mutant plants. Thus, via viral titer quantification experiments and in situ hybridization assays, we define the viral cycle steps that are altered by the absence of the m(6)A demethylase ALKBH9B in Arabidopsis. We found that ALKBH9A and ALKBH9C do not regulate the AMV cycle, so ALKBH9B activity seems to be highly specific. We also define that not only systemic movement is affected by the absence of the demethylase, but also early stages of viral infection. Moreover, our findings suggest that viral upload into the phloem could be blocked in alkbh9b plants. Overall, our results point to ALKBH9B as a possible new component of phloem transport, at least for AMV, and as a potential target to obtain virus resistance crops. | es_ES |
dc.description.sponsorship | This research was funded by the Spanish Agencia Estatal de Investigacion (AEI), grant numbers PID2020-115571RB-I00 to VP, and PGC2018-093445-B-I00 to JLM. MM-P was recipient of Predoctoral Contract FPI-2015-072406 from the Subprograma Formacion de Personal InvestigadorMinisterio de Economia y Competitividad (FPI-MINECO). LA-M was recipient of a Predoctoral contract from the Ministerio de Ciencia, Tecnologia y Telecomunicaciones from Costa Rica (MICITT-PINN-CON-624-2019). RN was recipient of the GRISOLIAP/2016/131 Predoctoral Contract from the Generalitat Valenciana. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Frontiers Media SA | es_ES |
dc.relation.ispartof | Frontiers in Microbiology | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | AMV | es_ES |
dc.subject | M6A | es_ES |
dc.subject | ALKBH9B | es_ES |
dc.subject | Systemic infection | es_ES |
dc.subject | Phloem transport | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | The m6A RNA demethylase ALKBH9B plays a critical role for vascular movement of Alfalfa mosaic virus in Arabidopsis | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3389/fmicb.2021.745576 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-093445-B-I00/ES/NUEVOS COMPONENTES DE LA MAQUINARIA EPIGENETICA DE LAS PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICITT//MICITT-PINN-CON-624-2019/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//GRISOLIAP%2F2016%2F131/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//BES-2015-072406/ES/BES-2015-072406/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia | es_ES |
dc.description.bibliographicCitation | Martínez-Pérez, M.; Gómez Mena, MC.; Alvarado-Marchena, L.; Nadi, R.; Micol, JL.; Pallás Benet, V.; Aparicio Herrero, F. (2021). The m6A RNA demethylase ALKBH9B plays a critical role for vascular movement of Alfalfa mosaic virus in Arabidopsis. Frontiers in Microbiology. 12:1-13. https://doi.org/10.3389/fmicb.2021.745576 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2021.745576 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 13 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 12 | es_ES |
dc.identifier.pmid | 34671333 | es_ES |
dc.identifier.pmcid | PMC8521051 | es_ES |
dc.relation.pasarela | S\446507 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica | es_ES |