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dc.contributor.author | VIDAURRE AGUT, CARLA MARIA | es_ES |
dc.contributor.author | Rivero-Buceta, Eva María | es_ES |
dc.contributor.author | Landry, Christopher C. | es_ES |
dc.contributor.author | BOTELLA ASUNCION, PABLO | es_ES |
dc.date.accessioned | 2022-07-29T18:02:12Z | |
dc.date.available | 2022-07-29T18:02:12Z | |
dc.date.issued | 2021-05 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/184972 | |
dc.description.abstract | [EN] To understand the factors that control the formation of the biomolecular corona, a systematic study of the adsorption of several miRNAs shown to be important in prostate cancer on amine-functionalized mesoporous silica nanoparticles (MSN-NH2) has been performed. Process parameters including miRNA type, nanoparticle concentration, incubation temperature and incubation time were investigated, as well as the potential competition for adsorption between different miRNA molecules. The influence of proteins and particle PEGylation on miRNA adsorption were also explored. We found that low particle concentrations and physiological temperature both led to increased miRNA adsorption. Adsorption of miRNA was also higher when proteins were present in the same solution; reducing or preventing protein adsorption by PEGylating the MSNs hindered adsorption. Finally, the amount of miRNA adsorbed from human serum by MSN-NH2 was compared to a commercial miRNA purification kit (TaqMan(R), Life Technologies, Carlsbad, CA, USA). MSN-NH2 adsorbed six times as much miRNA as the commercial kit, demonstrating higher sensitivity to subtle up- and downregulation of circulating miRNA in the blood of patients. | es_ES |
dc.description.sponsorship | This research was funded by the Spanish Ministry of Economy and Competitiveness (project PID2019-111436RB-C21), and the Generalitat Valenciana (project PROMETEO/2017/060). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Nanomaterials | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Mesoporous silica nanoparticles | es_ES |
dc.subject | Biomolecular corona | es_ES |
dc.subject | MiRNA | es_ES |
dc.subject | MiR-200c | es_ES |
dc.subject | MiR-221 | es_ES |
dc.subject | MiR-375 | es_ES |
dc.subject | Prostate cancer | es_ES |
dc.subject | Cancer diagnostic | es_ES |
dc.title | Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/nano11051196 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111436RB-C21/ES/IMAGEN FOTOACUSTICA CON NANOPARTICUTLAS CON PROPIEDADES OPTOELECTRONICAS Y MAGNETICAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2017%2F060//El pasado lejano: aproximación a la conducta y la ocupación del territorio en el Paleolítico valenciano/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química | es_ES |
dc.description.bibliographicCitation | Vidaurre Agut, CM.; Rivero-Buceta, EM.; Landry, CC.; Botella Asuncion, P. (2021). Isolation and Quantification of miRNA from the Biomolecular Corona on Mesoporous Silica Nanoparticles. Nanomaterials. 11(5):1-11. https://doi.org/10.3390/nano11051196 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/nano11051196 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 11 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 11 | es_ES |
dc.description.issue | 5 | es_ES |
dc.identifier.eissn | 2079-4991 | es_ES |
dc.identifier.pmid | 34062789 | es_ES |
dc.identifier.pmcid | PMC8147382 | es_ES |
dc.relation.pasarela | S\461576 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |