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Large-scale gene gains and losses molded the NLR defense arsenal during the Cucurbita evolution

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Large-scale gene gains and losses molded the NLR defense arsenal during the Cucurbita evolution

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dc.contributor.author Andolfo, Guisseppe es_ES
dc.contributor.author Sáez-Sánchez, Cristina es_ES
dc.contributor.author Cañizares Sales, Joaquín es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.contributor.author Ercolano, Maria R. es_ES
dc.date.accessioned 2022-09-05T18:03:06Z
dc.date.available 2022-09-05T18:03:06Z
dc.date.issued 2021-10 es_ES
dc.identifier.issn 0032-0935 es_ES
dc.identifier.uri http://hdl.handle.net/10251/185279
dc.description.abstract [EN] Main conclusion Genome-wide annotation reveals that the gene birth-death process of the Cucurbita R family is associated with a species-specific diversification of TNL and CNL protein classes. The Cucurbitaceae family includes nearly 1000 plant species known universally as cucurbits. Cucurbita genus includes many economically important worldwide crops vulnerable to more than 200 pathogens. Therefore, the identification of pathogen-recognition genes is of utmost importance for this genus. The major class of plant-resistance (R) genes encodes nucleotide-binding site and leucine-rich repeat (NLR) proteins, and is divided into three sub-classes namely, TIR-NB-LRR (TNL), CC-NB-LRR (CNL) and RPW8-NB-LRR (RNL). Although the characterization of the NLR gene family has been carried out in important Cucurbita species, this information is still linked to the availability of sequenced genomes. In this study, we analyzed 40 de novo transcriptomes and 5 genome assemblies, which were explored to investigate the Cucurbita expressed-NLR (eNLR) and NLR repertoires using an ad hoc gene annotation approach. Over 1850 NLR-encoding genes were identified, finely characterized and compared to 96 well-characterized plant R-genes. The maximum likelihood analyses revealed an unusual diversification of CNL/TNL genes and a strong RNL conservation. Indeed, several gene gain and loss events have shaped the Cucurbita NLR family. Finally, to provide a first validation step Cucurbita, eNLRs were explored by real-time PCR analysis. The NLR repertories of the 12 Cucurbita species presented in this paper will be useful to discover novel R-genes. es_ES
dc.description.sponsorship Open access funding provided by Universita degli Studi di Napoli Federico II within the CRUI-CARE Agreement. es_ES
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Planta es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Diversifying selection es_ES
dc.subject Orthology relations es_ES
dc.subject Phylogeny es_ES
dc.subject R-genes es_ES
dc.subject Transcriptomes es_ES
dc.subject.classification GENETICA es_ES
dc.title Large-scale gene gains and losses molded the NLR defense arsenal during the Cucurbita evolution es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00425-021-03717-x es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GENERALITAT VALENCIANA//PROMETEO%2F2017%2F078//SELECCION DE VARIEDADES TRADICIONALES Y DESARROLLO DE NUEVAS VARIEDADES DE CUCURBITACEAS ADAPTADAS A LA PRODUCCION ECOLOGICA./ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AGENCIA ESTATAL DE INVESTIGACION//RTA2017-00061-C03-03//AVANCES EN EL CONTROL DE LOS VIRUS TOLCNDV Y CGMMV EN CUCURBITACEAS MEDIANTE MEJORA GENÉTICA/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Andolfo, G.; Sáez-Sánchez, C.; Cañizares Sales, J.; Picó Sirvent, MB.; Ercolano, MR. (2021). Large-scale gene gains and losses molded the NLR defense arsenal during the Cucurbita evolution. Planta. 254(4):1-14. https://doi.org/10.1007/s00425-021-03717-x es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s00425-021-03717-x es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 14 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 254 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 34559316 es_ES
dc.identifier.pmcid PMC8463517 es_ES
dc.relation.pasarela S\455430 es_ES
dc.contributor.funder GENERALITAT VALENCIANA es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder Università degli Studi di Napoli Federico II es_ES
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dc.subject.ods 02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible es_ES


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