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dc.contributor.author | Juste-Dolz, Augusto Miguel | es_ES |
dc.contributor.author | Delgado-Pinar, Martina | es_ES |
dc.contributor.author | Avella-Oliver, Miquel | es_ES |
dc.contributor.author | Fernández-Sánchez, María Estrella | es_ES |
dc.contributor.author | Cruz, José Luis | es_ES |
dc.contributor.author | Andrés, Miguel V. | es_ES |
dc.contributor.author | Maquieira Catala, Angel | es_ES |
dc.date.accessioned | 2023-02-22T19:00:40Z | |
dc.date.available | 2023-02-22T19:00:40Z | |
dc.date.issued | 2022-09-14 | es_ES |
dc.identifier.issn | 1944-8244 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/192033 | |
dc.description.abstract | [EN] The nanostructuration of biolayers has become a paradigm for exploiting nanoscopic light-matter phenomena for biosensing, among other biomedical purposes. In this work, we present a photopatterning method to create periodic structures of biomacromolecules based on a local and periodic mild denaturation of protein biolayers mediated by UV-laser irradiation. These nanostructures are constituted by a periodic modulation of the protein activity, so they are free of topographic and compositional changes along the pattern. Herein, we introduce the approach, explore the patterning parameters, characterize the resulting structures, and assess their overall homogeneity. This UV-based patterning principle has proven to be an easy, cost-effective, and fast way to fabricate large areas of homogeneous one-dimensional protein patterns (2 min, 15 x 1.2 mm, relative standard deviation ? 16%). This work also investigates the implementation of these protein patterns as transducers for diffractive biosensing. Using a model immunoassay, these patterns have demonstrated negligible signal contributions from non-specific bindings and comparable experimental limits of detection in buffer media and in human serum (53 and 36 ng & BULL;mL-1 of unlabeled IgG, respectively). | es_ES |
dc.description.sponsorship | This work was financially supported by the Ministerio de Ciencia e Innovacion/Agencia Estatal de Investigacion (MCIN/AEI/10.13039/501100011033) , co-funded by the European Union "ERDF A way of making Europe", under grants PID2019-110713RB-I00 and PDI2019-104276RB-I00, and Generalitat Valenciana (PROMETEO/2020/094 and PROMETEO/2019/048) . A.J.-D. acknowledges the FPI-UPV 2017 grant program. The authors thank Angel Lopez Munoz for the construction of the scanning system. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | ACS Applied Materials & Interfaces | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | Biosensor | es_ES |
dc.subject | UV denaturation: Immunoassay | es_ES |
dc.subject | Non-specific binding | es_ES |
dc.subject | Label-free | es_ES |
dc.subject | Diffraction | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1021/acsami.2c12808 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104276RB-I00/ES/ONDAS ACUSTICAS EN FIBRAS OPTICAS Y MICROCAVIDADES/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/UPV//FPI 2017/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110713RB-I00/ES/HACIA EL BIOSENSADO HOLOGRAFICO AVANZADO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2020%2F094//HOLOGRAFÍA. UNA VIA PARA AFRONTAR NUEVOS RETOS EN BIOSENSADO/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/GVA//PROMETEO%2F2019%2F048/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Juste-Dolz, AM.; Delgado-Pinar, M.; Avella-Oliver, M.; Fernández-Sánchez, ME.; Cruz, JL.; Andrés, MV.; Maquieira Catala, A. (2022). Denaturing for Nanoarchitectonics: Local and Periodic UV-Laser Photodeactivation of Protein Biolayers to Create Functional Patterns for Biosensing. ACS Applied Materials & Interfaces. 14(36):41640-41648. https://doi.org/10.1021/acsami.2c12808 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1021/acsami.2c12808 | es_ES |
dc.description.upvformatpinicio | 41640 | es_ES |
dc.description.upvformatpfin | 41648 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 14 | es_ES |
dc.description.issue | 36 | es_ES |
dc.identifier.pmid | 36047566 | es_ES |
dc.identifier.pmcid | PMC9940103 | es_ES |
dc.relation.pasarela | S\479144 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | European Regional Development Fund | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |