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dc.contributor.author | Alvarado-Marchena, Luis | es_ES |
dc.contributor.author | Martínez-Pérez, Mireya | es_ES |
dc.contributor.author | Úbeda, Jesús R. | es_ES |
dc.contributor.author | Pallás Benet, Vicente | es_ES |
dc.contributor.author | APARICIO HERRERO, FREDERIC | es_ES |
dc.date.accessioned | 2023-06-15T18:00:37Z | |
dc.date.available | 2023-06-15T18:00:37Z | |
dc.date.issued | 2022-08 | es_ES |
dc.identifier.issn | 1999-4915 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/194257 | |
dc.description.abstract | [EN] We have previously reported the presence of m(6)A in the AMV (Alfamovirus, Bromoviridae) genome. Interestingly, two of these putative m(6)A-sites are in hairpin (hp) structures in the 3'UTR of the viral RNA3. One site ((2012)AAACU(2016)) is in the loop of hpB, within the coat protein binding site 1 (CPB1), while the other ((1900)UGACC(1904)) is in the lower stem of hpE, a loop previously associated with AMV negative-strand RNA synthesis. In this work, we have performed in vivo experiments to assess the role of these two regions, containing the putative m(6)A-sites in the AMV cycle, by introducing compensatory point mutations to interfere with or abolish the m(6)A-tag of these sites. Our results suggest that the loop of hpB could be involved in viral replication/accumulation. Meanwhile, in the (1900)UGACC(1904) motif of the hpE, the maintenance of the adenosine residue and the lower stem hpE structure are necessary for in vivo plus-strand accumulation. These results extend our understanding of the requirements for hpE in the AMV infection cycle, indicating that both the residue identity and the base-pairing capacity in this structure are essential for viral accumulation. | es_ES |
dc.description.sponsorship | This research was funded by grant PID2020-115571RB-I00 to F.A and V.P., from the Spanish MCIN/AEI/10.13039/501100011033 granting agency. L.A.-M. was the recipient of a predoctoral contract from the Ministerio de Ciencia, Tecnologia y Telecomunicaciones, from Costa Rica (MICITTPINN-CON-624-2019). | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | MDPI AG | es_ES |
dc.relation.ispartof | Viruses | es_ES |
dc.rights | Reconocimiento (by) | es_ES |
dc.subject | N-6-methyladenosine | es_ES |
dc.subject | RNA covalent modifications | es_ES |
dc.subject | Plant alfamovirus | es_ES |
dc.subject | DRACH motif | es_ES |
dc.subject | In vivo AMV replication | es_ES |
dc.subject | 3 ' UTR | es_ES |
dc.subject.classification | BIOQUIMICA Y BIOLOGIA MOLECULAR | es_ES |
dc.title | Impact of the Potential m(6) A Modification Sites at the 3 ' UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.3390/v14081718 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-115571RB-I00/ES/LA MODIFICACION N6-METILADENOSINA (M6A) DEL RNA Y EL DIRECCIONAMIENTO DUAL A CLOROPLASTOS Y MITOCONDRIAS COMO MECANISMOS REGULADORES EN LA BIOLOGIA DE LOS VIRUS RNA DE PLANTAS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICITT//MICITT-PINN-CON-624-2019/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Alvarado-Marchena, L.; Martínez-Pérez, M.; Úbeda, JR.; Pallás Benet, V.; Aparicio Herrero, F. (2022). Impact of the Potential m(6) A Modification Sites at the 3 ' UTR of Alfalfa Mosaic Virus RNA3 in the Viral Infection. Viruses. 14(8):1-10. https://doi.org/10.3390/v14081718 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.3390/v14081718 | es_ES |
dc.description.upvformatpinicio | 1 | es_ES |
dc.description.upvformatpfin | 10 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 14 | es_ES |
dc.description.issue | 8 | es_ES |
dc.identifier.pmid | 36016339 | es_ES |
dc.identifier.pmcid | PMC9414508 | es_ES |
dc.relation.pasarela | S\482711 | es_ES |
dc.contributor.funder | Agencia Estatal de Investigación | es_ES |
dc.contributor.funder | Ministerio de Ciencia Tecnología y Telecomunicaciones de Costa Rica | es_ES |