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dc.contributor.author | Alfaro Fernández, Ana Olvido | es_ES |
dc.contributor.author | Cebrián, M.C | es_ES |
dc.contributor.author | Herrera-Vasquez, J. A. | es_ES |
dc.contributor.author | Córdoba-Sellés, M. C. | es_ES |
dc.contributor.author | SANCHEZ NAVARRO, JESUS ANGEL | es_ES |
dc.contributor.author | Jordá, C. | es_ES |
dc.date.accessioned | 2024-01-18T19:01:02Z | |
dc.date.available | 2024-01-18T19:01:02Z | |
dc.date.issued | 2010-08 | es_ES |
dc.identifier.issn | 0032-0862 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/202019 | |
dc.description.abstract | [EN] The population structure and genetic variation of Tomato torrado virus (ToTV) were estimated from 19 Spanish isolates collected from 2001 to 2009 in different tomato-production areas by analyses of the partial nucleotide sequences of five regions of the virus genome: the protease cofactor (Pro-Co) and the RNA-dependent RNA polymerase (RdRp) in RNA1, and the movement protein (MP) and two subunits of the coat protein (CP; viz. Vp35 and Vp23) in RNA2. Three Hungarian isolates of the virus were also included in the analyses. All the ToTV isolates clustered together in the phylogenetic analysis of the nucleotide sequences of the different regions. However, some genetic diversity was observed in the case of the two CP subunits among the Gran Canaria isolates and the remaining ToTV-isolates analysed, which grouped together. A high similarity was observed among all the isolates and the two published ToTV isolates: the ToTV type isolate (PRI-ToTV0301) and the Polish isolate Wal03. The most variable encoding regions studied were those on RNA2. In general, no correlation was found between genetic diversity and collection date. Studying the genetic distances between pairs of sequences, the ratio between nonsynonymous (amino-acid-replacing) and synonymous (silent mutational) substitutions was low, indicating a strong negative selective pressure in the studied regions. Nine negatively selected sites (distributed in Pro-Co, MP, Vp23 and Vp35) and just one positively selected one (in Pro-Co) were found for all the genome regions studied. | es_ES |
dc.description.sponsorship | This work was supported by grants AGL2005-06682-C03-01 from the Spanish Ministry of Education and Science (MEC). We thank the Instituto Agroforestal Mediterraneo (UPV, Valencia) for fellowship support to AAF. We also thank Dr M. Juarez (Universidad Miguel Hernandez, Orihuela) and Dr A. Lacasa (IMI-DA, Murcia) for their advice and assistance in the field surveys. We wish to also thank Bese Gabor (Csongrad Megyei MgSzH) and A. Forray (Floraton Kft.) for providing the Hungarian isolates of ToTV included in this assay. | es_ES |
dc.language | Inglés | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.relation.ispartof | Plant Pathology | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Genetic variation | es_ES |
dc.subject | Phylogenetic analysis | es_ES |
dc.subject | RNA sequence | es_ES |
dc.subject | Solanum lycopersicum | es_ES |
dc.subject | Weeds | es_ES |
dc.subject.classification | PRODUCCION VEGETAL | es_ES |
dc.title | Molecular variability of Spanish and Hungarian isolates of Tomato torrado virus | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1111/j.1365-3059.2010.02280.x | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//AGL2005-06682-C03-01/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural | es_ES |
dc.description.bibliographicCitation | Alfaro Fernández, AO.; Cebrián, M.; Herrera-Vasquez, JA.; Córdoba-Sellés, MC.; Sanchez Navarro, JA.; Jordá, C. (2010). Molecular variability of Spanish and Hungarian isolates of Tomato torrado virus. Plant Pathology. 59(4):785-793. https://doi.org/10.1111/j.1365-3059.2010.02280.x | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://doi.org/10.1111/j.1365-3059.2010.02280.x | es_ES |
dc.description.upvformatpinicio | 785 | es_ES |
dc.description.upvformatpfin | 793 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 59 | es_ES |
dc.description.issue | 4 | es_ES |
dc.relation.pasarela | S\39139 | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |
dc.contributor.funder | Universitat Politècnica de València | es_ES |