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Multiplex PCR assay for the simultaneous detection and differentiation of Olpidium bornovanus, O. brassicae, and O. virulentus

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Multiplex PCR assay for the simultaneous detection and differentiation of Olpidium bornovanus, O. brassicae, and O. virulentus

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dc.contributor.author Herrera-Vásquez, Jose Angel es_ES
dc.contributor.author Cebrián, María del Carmen es_ES
dc.contributor.author Alfaro Fernández, Ana Olvido es_ES
dc.contributor.author Córdoba-Sellés, Maria del Carmen es_ES
dc.contributor.author Jordá, Concepción es_ES
dc.date.accessioned 2024-01-18T19:01:15Z
dc.date.available 2024-01-18T19:01:15Z
dc.date.issued 2009-05 es_ES
dc.identifier.issn 0953-7562 es_ES
dc.identifier.uri http://hdl.handle.net/10251/202023
dc.description.abstract [EN] A multiplex PCR method has been developed to detect, differentiate, and confirm the morphological identification of three root infecting Olpidium spp.: O. bornovanus, O. brassicae, and O. virulentus. Of the 132 root samples examined, 101 samples were infected by Olpidium spp.. Based on the morphology of resting spores, the presence of O. bornovanus was confirmed in 20.5 % of the samples, whereas species identity could not be determined for the remaining samples because they failed to reproduce sexually. With multiplex PCR, it was possible to determine the Olpidium identity of all the infected samples, even when resting spores were not formed. This method was also effective for detecting Olpidium spp. in water samples. In addition, the specificity and sensitivity of multiplex PCR were evaluated. The multiplex PCR method was validated with samples of 9 different crops from 11 countries of America, Europe, and Africa. es_ES
dc.description.sponsorship Jose Angel Herrera Vasquez was supported by a predoctoral fellowship from the Agencia Espanola de Cooperacion Internacional Para el Desarrollo (AECID, Spain, 2004-2007 period) and the Instituto Para la Formacion y Aprovechamiento de Recursos Humanos and the Secretaria Nacional de Ciencia y Tecnologia (IFARHU-SENACYT, Panama, 2007-2010 period). We thank the Direccion Nacional de Sanidad Vegetal-Ministerio de Desarrollo Agropecuario (DNSV-MIDA, Panama) for their technical assistance. We also thank Dr. M. L Herrero (Bioforsk, Plant Health and Plant Protection Division, Norway) and Dr. J. C. Tello (Plant Production Department, University of Almeria, Spain) for providing the fungal isolates used in the specificity assays, and Dr. R. Beltran for providing the soil samples from Brazil. This work was supported by grants AGL 2005-06682-C03-01 from the Comision Interministerial de Ciencia y Tecnologia CICYT, Spain. es_ES
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation.ispartof Mycological Research es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Chytrid species es_ES
dc.subject Molecular identification es_ES
dc.subject Morphological identification es_ES
dc.subject Sensitivity es_ES
dc.subject Specificity es_ES
dc.subject.classification PRODUCCION VEGETAL es_ES
dc.title Multiplex PCR assay for the simultaneous detection and differentiation of Olpidium bornovanus, O. brassicae, and O. virulentus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.mycres.2009.01.007 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MEC//AGL2005-06682-C03-01/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural - Escola Tècnica Superior d'Enginyeria Agronòmica i del Medi Natural es_ES
dc.description.bibliographicCitation Herrera-Vásquez, JA.; Cebrián, MDC.; Alfaro Fernández, AO.; Córdoba-Sellés, MDC.; Jordá, C. (2009). Multiplex PCR assay for the simultaneous detection and differentiation of Olpidium bornovanus, O. brassicae, and O. virulentus. Mycological Research. 113:602-610. https://doi.org/10.1016/j.mycres.2009.01.007 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.mycres.2009.01.007 es_ES
dc.description.upvformatpinicio 602 es_ES
dc.description.upvformatpfin 610 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 113 es_ES
dc.identifier.pmid 19640401 es_ES
dc.relation.pasarela S\37264 es_ES
dc.contributor.funder Ministerio de Educación y Ciencia es_ES
dc.contributor.funder Agencia Española de Cooperación Internacional para el Desarrollo es_ES
dc.contributor.funder Secretaría Nacional de Ciencia, Tecnología e Innovación, Panamá es_ES


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