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Multicolor plate reader fluorescence calibration

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Multicolor plate reader fluorescence calibration

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dc.contributor.author Beal, Jacob es_ES
dc.contributor.author Telmer, Cheryl A. es_ES
dc.contributor.author Vignoni, Alejandro es_ES
dc.contributor.author Boada-Acosta, Yadira Fernanda es_ES
dc.contributor.author Baldwin, Geoff S. es_ES
dc.contributor.author Hallett, Liam es_ES
dc.contributor.author Lee, Taeyang es_ES
dc.contributor.author Selvarajah, Vinoo es_ES
dc.contributor.author Billerbeck, Sonja es_ES
dc.contributor.author Brown, Bradley es_ES
dc.contributor.author Cai, Guo-nan es_ES
dc.contributor.author Cai, Liang es_ES
dc.contributor.author Eisenstein, Edward es_ES
dc.contributor.author Kiga, Daisuke es_ES
dc.contributor.author Ross, David es_ES
dc.date.accessioned 2024-04-24T18:06:18Z
dc.date.available 2024-04-24T18:06:18Z
dc.date.issued 2022-02-02 es_ES
dc.identifier.uri http://hdl.handle.net/10251/203726
dc.description This is a pre-copyedited, author-produced version of an article accepted for publication in Synthetic Biology following peer review. The version of record Jacob Beal, Cheryl A Telmer, Alejandro Vignoni, Yadira Boada, Geoff S Baldwin, Liam Hallett, Taeyang Lee, Vinoo Selvarajah, Sonja Billerbeck, Bradley Brown, Guo-nan Cai, Liang Cai, Edward Eisenstein, Daisuke Kiga, David Ross, Nina Alperovich, Noah Sprent, Jaclyn Thompson, Eric M Young, Drew Endy, Traci Haddock-Angelli, Multicolor plate reader fluorescence calibration, Synthetic Biology, Volume 7, Issue 1, 2022, ysac010, https://doi.org/10.1093/synbio/ysac010, is available online at: https://doi.org/10.1093/synbio/ysac010. es_ES
dc.description.abstract [EN] Plate readers are commonly used to measure cell growth and fluorescence, yet the utility and reproducibility of plate reader data is limited by the fact that it is typically reported in arbitrary or relative units. We have previously established a robust serial dilution protocol for calibration of plate reader measurements of absorbance to estimated bacterial cell count and for green fluorescence from proteins expressed in bacterial cells to molecules of equivalent fluorescein. We now extend these protocols to calibration of red fluorescence to the sulforhodamine-101 fluorescent dye and blue fluorescence to Cascade Blue. Evaluating calibration efficacy via an interlaboratory study, we find that these calibrants do indeed provide comparable precision to the prior calibrants and that they enable effective cross-laboratory comparison of measurements of red and blue fluorescence from proteins expressed in bacterial cells. es_ES
dc.description.sponsorship This work was supported in part by the following funding sources: J.B. was supported by Air Force Research Laboratory (AFRL) and DARPA contract FA8750-17-C-0184. N.S. was supported by funding from the BBSRC under award BB/M011178/1. G.B., L.H., and T.L. were supported by the EPSRC under award EP/R034915/1 and EP/S022856/1. G.C. and L.C. were supported by funds from YF Capital and the National Top Talent Undergraduate Training Program, China. A.V. and Y.B. were funded by Grant MINECO/AEI, EU DPI2017-82896-C2-1-R and MCIN/AEI/10.13039/501100011033 grant number PID2020117271RB-C21. Y.B. was supported by Secretaria de Educaci ' on Superior, Ciencia, Tecnologia e Innovacion-Ecuador (Scholarship Convocatoria Abierta 2011). D.K. was supported by JST, CREST Grant Number JPMJCR21N4, Japan. E.Y. was supported by the National Science Foundation under Grant No. 1939860. J.T. was supported by the Office of the Director of National Intelligence (ODNI), Intelligence Advanced Research Projects Activity (IARPA) under Finding Engineering Linked Indicators (FELIX) program contract N66001-18-C-4507. This document does not contain technology or technical data controlled under either the U.S. International Traffic in Arms Regulations or the U.S. Export Administration Regulations. Views, opinions, and/or findings expressed are those of the author(s) and should not be interpreted as representing the official views or policies of the Department of Defense or the U.S. Government. es_ES
dc.language Inglés es_ES
dc.publisher Oxford University Press es_ES
dc.relation.ispartof Synthetic Biology es_ES
dc.rights Reconocimiento - No comercial (by-nc) es_ES
dc.subject Calibration es_ES
dc.subject Units es_ES
dc.subject Fluorescence es_ES
dc.subject Cell count es_ES
dc.subject.classification INGENIERIA DE SISTEMAS Y AUTOMATICA es_ES
dc.title Multicolor plate reader fluorescence calibration es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1093/synbio/ysac010 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AEI//DPI2017-82896-C2-1-R-AR//DISEÑO, CARACTERIZACION Y AJUSTE OPTIMO DE BIOCIRCUITOS SINTETICOS PARA BIOPRODUCCION CON CONTROL DE CARGA METABOLICA/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/AGENCIA ESTATAL DE INVESTIGACION//PID2020-117271RB-C21//REGULACIÓN DINÁMICA MULTI-ESCALA EN INGENIERÍA METABÓLICA: CONTROL REALIMENTADO Y CICLO DBTL AUTOMATIZADO/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/NSF//1939860/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EPSRC//EP%2FR034915%2F1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EPSRC//EP%2FS022856%2F1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/JST//JPMJCR21N4/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BBSRC//BB%2FM011178%2F1/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/DARPA//FA8750-17-C-0184/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/IARPA//N66001-18-C-4507//Finding Engineering Linked Indicators (FELIX)/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Escuela Técnica Superior de Ingeniería del Diseño - Escola Tècnica Superior d'Enginyeria del Disseny es_ES
dc.description.bibliographicCitation Beal, J.; Telmer, CA.; Vignoni, A.; Boada-Acosta, YF.; Baldwin, GS.; Hallett, L.; Lee, T.... (2022). Multicolor plate reader fluorescence calibration. Synthetic Biology. 7(1):1-9. https://doi.org/10.1093/synbio/ysac010 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1093/synbio/ysac010 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 9 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 7 es_ES
dc.description.issue 1 es_ES
dc.identifier.eissn 2397-7000 es_ES
dc.identifier.pmid 35949424 es_ES
dc.identifier.pmcid PMC9357555 es_ES
dc.relation.pasarela S\486332 es_ES
dc.contributor.funder Yunfeng Capital es_ES
dc.contributor.funder Air Force Research Laboratory es_ES
dc.contributor.funder AGENCIA ESTATAL DE INVESTIGACION es_ES
dc.contributor.funder National Science Foundation, EEUU es_ES
dc.contributor.funder Japan Science and Technology Agency es_ES
dc.contributor.funder Defense Advanced Research Projects Agency es_ES
dc.contributor.funder Office of the Director of National Intelligence es_ES
dc.contributor.funder Intelligence Advanced Research Projects Activity es_ES
dc.contributor.funder Engineering and Physical Sciences Research Council, Reino Unido es_ES
dc.contributor.funder Biotechnology and Biological Sciences Research Council, Reino Unido es_ES
dc.contributor.funder Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Ecuador es_ES


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