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Root transcriptional responses of two melongenotypes with contrasting resistance toMonosporascus cannonballus (Pollack et Uecker)infection

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Root transcriptional responses of two melongenotypes with contrasting resistance toMonosporascus cannonballus (Pollack et Uecker)infection

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dc.contributor.author Roig Montaner, Mª Cristina es_ES
dc.contributor.author Fita, Ana es_ES
dc.contributor.author Rios, Gabino es_ES
dc.contributor.author Hammond, John P. es_ES
dc.contributor.author Nuez Viñals, Fernando es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.date.accessioned 2013-09-03T13:33:59Z
dc.date.available 2013-09-03T13:33:59Z
dc.date.issued 2012
dc.identifier.issn 1471-2164
dc.identifier.uri http://hdl.handle.net/10251/31713
dc.description.abstract Background: Monosporascus cannonballus is the main causal agent of melon vine decline disease. Several studies have been carried out mainly focused on the study of the penetration of this pathogen into melon roots, the evaluation of symptoms severity on infected roots, and screening assays for breeding programs. However, a detailed molecular view on the early interaction between M. cannonballus and melon roots in either susceptible or resistant genotypes is lacking. In the present study, we used a melon oligo-based microarray to investigate the gene expression responses of two melon genotypes, Cucumis melo ¿Piel de sapo¿ (¿PS¿) and C. melo ¿Pat 81¿, with contrasting resistance to the disease. This study was carried out at 1 and 3 days after infection (DPI) by M. cannonballus. Results: Our results indicate a dissimilar behavior of the susceptible vs. the resistant genotypes from 1 to 3 DPI. ¿PS¿ responded with a more rapid infection response than ¿Pat 81¿ at 1 DPI. At 3 DPI the total number of differentially expressed genes identified in ¿PS¿ declined from 451 to 359, while the total number of differentially expressed transcripts in ¿Pat 81¿ increased from 187 to 849. Several deregulated transcripts coded for components of Ca2+ and jasmonic acid (JA) signalling pathways, as well as for other proteins related to defence mechanisms. Transcriptional differences in the activation of the JA-mediated response in ¿Pat 81¿ compared to ¿PS¿ suggested that JA response might be partially responsible for their observed differences in resistance. Conclusions: As a result of this study we have identified for the first time a set of candidate genes involved in the root response to the infection of the pathogen causing melon vine decline. This information is useful for understanding the disease progression and resistance mechanisms few days after inoculation. es_ES
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Genomics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject.classification GENETICA es_ES
dc.title Root transcriptional responses of two melongenotypes with contrasting resistance toMonosporascus cannonballus (Pollack et Uecker)infection es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/1471-2164-13-601
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Roig Montaner, MC.; Fita, A.; Rios, G.; Hammond, JP.; Nuez Viñals, F.; Picó Sirvent, MB. (2012). Root transcriptional responses of two melongenotypes with contrasting resistance toMonosporascus cannonballus (Pollack et Uecker)infection. BMC Genomics. 13(601):1-12. doi:10.1186/1471-2164-13-601 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1186/1471-2164-13-601 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 12 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 13 es_ES
dc.description.issue 601 es_ES
dc.relation.senia 234253
dc.identifier.pmid 23134692 en_EN
dc.identifier.pmcid PMC3542287 en_EN
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