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Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.)

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Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.)

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dc.contributor.author Leida, Carmen es_ES
dc.contributor.author Moser, Claudio es_ES
dc.contributor.author Esteras Gómez, Cristina es_ES
dc.contributor.author Sulpice, Ronan es_ES
dc.contributor.author Lunn, John E. es_ES
dc.contributor.author Lagen, Frank de es_ES
dc.contributor.author Monforte Gilabert, Antonio José es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.date.accessioned 2016-05-13T13:08:10Z
dc.date.available 2016-05-13T13:08:10Z
dc.date.issued 2015-03-19
dc.identifier.issn 1471-2156
dc.identifier.uri http://hdl.handle.net/10251/64035
dc.description.abstract [EN] Background A collection of 175 melon (Cucumis melo L.) accessions (including wild relatives, feral types, landraces, breeding lines and commercial cultivars) from 50 countries was selected to study the phenotypic variability for ripening behavior and sugar accumulation. The variability of single nucleotide polymorphisms (SNPs) at 53 selected candidate genes involved in sugar accumulation and fruit ripening processes was studied, as well as their association with phenotypic variation of related traits. Results The collection showed a strong genetic structure, defining seven groups plus a number of accessions that could not be associated to any of the groups (admixture), which fitted well with the botanical classification of melon varieties. The variability in candidate genes for ethylene, cell wall and sugar-related traits was high and similar to SNPs located in reference genes. Variability at ripening candidate genes had an important weight on the genetic stratification of melon germplasm, indicating that traditional farmers might have selected for ripening traits during cultivar diversification. A strong relationship was also found between the genetic structure and phenotypic diversity, which could hamper genetic association studies. Accessions belonging to the ameri group are the most appropriate for association analysis given the high phenotypic and molecular diversity within the group, and lack of genetic structure. The most remarkable association was found between sugar content and SNPs in LG III, where a hotspot of sugar content QTLs has previously been defined. By studying the differences in allelic variation of SNPs within horticultural groups with specific phenotypic features, we also detected differential variation in sugar-related candidates located in LGIX and LGX, and in ripening-related candidates located in LGII and X, all in regions with previously mapped QTLs for the corresponding traits. Conclusions In the current study we have found an important variability at both the phenotypic and candidate gene levels for ripening behavior and sugar accumulation in melon fruit. By combination of differences in allelic diversity and association analysis, we have identified several candidate genes that may be involved in the melon phenotypic diversity. es_ES
dc.description.sponsorship C.L. is recipient of a Marie Curie Career Integration Grant (CIG) and her visit to IBMCP-COMAV (Valencia) is supported by ESF Cost Action "FA1106 Quality Fruit" - Short-Term Scientific Mission (STSM). We thank Dr. Pau Javier-Montero (COMAV) and Dr. Javier Forment Millet (IBMCP) for their kind bioinformatics support, Nicole Krohn and Beatrice Encke (MPIMP) for their technical support in sugar and malic acid analyses, and Prof. Mark Stitt for helpful discussions. Genotyping was supported by SAFQIM project, AGL2012-40130-C02-02 from the Spanish Ministry of Economy and Competitivity (MINECO). Metabolite analysis at the MPIMP was supported by the German Federal Ministry of Education and Research (FP6 ERA-NET Plant Genomics project MELRIP; BMBF contract 0313987 to Mark Stitt). en_EN
dc.language Inglés es_ES
dc.publisher BioMed Central es_ES
dc.relation.ispartof BMC Genetics es_ES
dc.rights Reconocimiento (by) es_ES
dc.subject Germplasm collection es_ES
dc.subject Sugar es_ES
dc.subject Climacteric ripening es_ES
dc.subject Melon es_ES
dc.subject.classification GENETICA es_ES
dc.title Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.) es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1186/s12863-015-0183-2
dc.relation.projectID info:eu-repo/grantAgreement/COST//FA1106/EU/Quality fruit/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//AGL2012-40130-C02-02/ES/DESCIFRANDO LA BASE GENETICA DE LA MORFOLOGIA DEL FRUTO Y LA DOMESTICACION DE MELON/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/BMBF//0313987/ es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Leida, C.; Moser, C.; Esteras Gómez, C.; Sulpice, R.; Lunn, JE.; Lagen, FD.; Monforte Gilabert, AJ.... (2015). Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon (Cucumis melo L.). BMC Genetics. 16(28):1-27. https://doi.org/10.1186/s12863-015-0183-2 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://dx.doi.org/10.1186/s12863-015-0183-2 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 27 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 16 es_ES
dc.description.issue 28 es_ES
dc.relation.senia 284195 es_ES
dc.identifier.pmid 25886993 en_EN
dc.identifier.pmcid PMC4380257 en_EN
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.contributor.funder European Cooperation in Science and Technology es_ES
dc.contributor.funder Bundesministerium für Bildung und Forschung, Alemania es_ES
dc.contributor.funder European Commission
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