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dc.contributor.author | Santiago Felipe, Sara | es_ES |
dc.contributor.author | Tortajada-Genaro, Luis Antonio | es_ES |
dc.contributor.author | Puchades, Rosa | es_ES |
dc.contributor.author | Maquieira Catala, Ángel | es_ES |
dc.date.accessioned | 2016-06-06T13:20:25Z | |
dc.date.available | 2016-06-06T13:20:25Z | |
dc.date.issued | 2014-02-06 | |
dc.identifier.issn | 0003-2670 | |
dc.identifier.uri | http://hdl.handle.net/10251/65319 | |
dc.description.abstract | [EN] Polymerase chain reaction in conjunction with enzyme-linked immunosorbent assay (PCR-ELISA) is a well-established technique that provides a suitable rapid, sensitive, and selective method for a broad range of applications. However, the need for precise rapid temperature cycling of PCR is an important drawback that can be overcome by employing isothermal amplification reactions such as recombinase polymerase amplification (RPA). The RPA-ELISA combination is proposed for amplification at a low, constant temperature (40 degrees C) in a short time (40 min), for the hybridisation of labelled products to specific 5'-biotinylated probes/streptavidin in coated microtiter plates at room temperature, and for detection by colorimetric immunoassay. RPA-ELISA was applied to screen common safety threats in foodstuffs, such as allergens (hazelnut, peanut, soybean, tomato, and maize), genetically modified organisms (P35S and TNOS), pathogenic bacteria (Salmonella sp. and Cronobacter sp.), and fungi (Fusarium sp.). Satisfactory sensitivity and reproducibility results were achieved for all the targets. The RPA-ELISA technique does away with thermocycling and provides a suitable sensitive, specific, and cost-effective method for routine applications, and proves particularly useful for resource-limited settings. (C) 2013 Elsevier B.V. All rights reserved. | es_ES |
dc.description.sponsorship | This research has been funded through Projects GV/2009/028 (Generalitat Valenciana) and CTQ/2010/15943 (MICINN). The Spanish Ministry of Education and Science provided S.S.F. with a grant for her PhD studies. The fungal cultures were supplied by B. Mora-Sala and J. Garcia-Jimenez from the Instituto Agroforestal Mediterraneo, UPV. | en_EN |
dc.language | Inglés | es_ES |
dc.publisher | Elsevier | es_ES |
dc.relation.ispartof | Analytica Chimica Acta | es_ES |
dc.rights | Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) | es_ES |
dc.subject | Isothermal amplification | es_ES |
dc.subject | ELISA | es_ES |
dc.subject | Allergen | es_ES |
dc.subject | GMO | es_ES |
dc.subject | Pathogen | es_ES |
dc.subject | Food safety | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1016/j.aca.2013.12.017 | |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//GV%2F2009%2F028/ES/Puesta a punto de biosensores en disco compacto/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICINN//CTQ2010-15943/ES/ESTUDIO DE NUEVAS VIAS DE DESARROLLO DE BIOMEMS PARA SCREENING MASIVO. DEMOSTRACION DE CONCEPTO COMO HERRAMIENTA DE ANALISIS APLICABLE EN "OMICAS"/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Santiago Felipe, S.; Tortajada-Genaro, LA.; Puchades, R.; Maquieira Catala, Á. (2014). Recombinase polymerase and enzyme-linked immunosorbent assay as a DNA amplification-detection strategy for food analysis. Analytica Chimica Acta. 811:81-87. https://doi.org/10.1016/j.aca.2013.12.017 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | https://dx.doi.org/10.1016/j.aca.2013.12.017 | es_ES |
dc.description.upvformatpinicio | 81 | es_ES |
dc.description.upvformatpfin | 87 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 811 | es_ES |
dc.relation.senia | 255420 | es_ES |
dc.identifier.eissn | 1873-4324 | |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Ciencia e Innovación | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |