Antoni-Alandes, R.; Gonzalez Guzman, M.; Rodriguez, L.; Peirats-Llobet, M.; Pizzio Bianchi, GA.; Fernández, MA.; De Winne, N.... (2013). PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root. Plant Physiology. 161(2):931-941. doi:10.1104/pp.112.208678
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/82494
Título:
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PYRABACTIN RESISTANCE1-LIKE8 plays an important role for the regulation of abscisic acid signaling in root
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Autor:
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Antoni-Alandes, Regina
González Guzmán, Miguel
Rodriguez, Lesia
Peirats-Llobet, Marta
Pizzio Bianchi, Gaston Alfredo
Fernández, Maria A.
De Winne, Nancy
De Jaeger, Geert
Dietrich, Daniela
Bennett, Malcolm J.
Rodríguez Egea, Pedro Luís
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Entidad UPV:
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Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
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Fecha difusión:
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Resumen:
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[EN] Abscisic acid (ABA) signaling plays a critical role in regulating root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival ...[+]
[EN] Abscisic acid (ABA) signaling plays a critical role in regulating root growth and root system architecture. ABA-mediated growth promotion and root tropic response under water stress are key responses for plant survival under limiting water conditions. In this work, we have explored the role of Arabidopsis (Arabidopsis thaliana) PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS for root ABA signaling. As a result, we discovered that PYL8 plays a nonredundant role for the regulation of root ABA sensitivity. Unexpectedly, given the multigenic nature and partial functional redundancy observed in the PYR/PYL family, the single pyl8 mutant showed reduced sensitivity to ABA-mediated root growth inhibition. This effect was due to the lack of PYL8-mediated inhibition of several clade A phosphatases type 2C (PP2Cs), since PYL8 interacted in vivo with at least five PP2Cs, namely HYPERSENSITIVE TO ABA1 (HAB1), HAB2, ABA-INSENSITIVE1 (ABI1), ABI2, and PP2CA/ABA-HYPERSENSITIVE GERMINATION3 as revealed by tandem affinity purification and mass spectrometry proteomic approaches. We also discovered that PYR/PYL receptors and clade A PP2Cs are crucial for the hydrotropic response that takes place to guide root growth far from regions with low water potential. Thus, an ABA-hypersensitive pp2c quadruple mutant showed enhanced hydrotropism, whereas an ABA-insensitive sextuple pyr/pyl mutant showed reduced hydrotropic response, indicating that ABA-dependent inhibition of PP2Cs by PYR/PYLs is required for the proper perception of a moisture gradient.
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Palabras clave:
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Tandem Affinity Purification (TAP)
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Phosphatases type 2C
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Different phosphorylation mechanisms
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Arabidopsis thaliana
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Protein phosphatases
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Growth maintenance
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Gene-expression
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Kinases
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Hydrotropism
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Derechos de uso:
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Reserva de todos los derechos
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Fuente:
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Plant Physiology. (issn:
0032-0889
) (eissn:
1532-2548
)
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DOI:
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10.1104/pp.112.208678
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Editorial:
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American Society of Plant Biologists
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Versión del editor:
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http://doi.org/10.1104/pp.112.208678
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Código del Proyecto:
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info:eu-repo/grantAgreement/MICINN//BIO2011-23446/ES/SEÑALIZACION DE ABA MEDIADA POR LOS RECEPTORES PYR%2FPYL Y SU CONEXION CON LOS MECANISMOS DE RESISTENCIA A SEQUIA/
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Agradecimientos:
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This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (grant no. BIO2011-23446 to P. L. R.; fellowships to R. A., L. ...[+]
This work was supported by the Ministerio de Ciencia e Innovacion, Fondo Europeo de Desarrollo Regional, and Consejo Superior de Investigaciones Cientificas (grant no. BIO2011-23446 to P. L. R.; fellowships to R. A., L. R., and M. P.-L.; Juan de la Cierva contract to M.G.-G.).
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Tipo:
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Artículo
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