- -

New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus

RiuNet: Repositorio Institucional de la Universidad Politécnica de Valencia

Compartir/Enviar a

Citas

Estadísticas

  • Estadisticas de Uso

New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus

Mostrar el registro sencillo del ítem

Ficheros en el ítem

[Cerrado]
Nombre: Rubio-Moraga;Ramb ...
Tamaño: 4.460Mb
Formato: PDF
Descripción: Versión editorial
Solicitar una copia al autor
dc.contributor.author Rubio-Moraga, Angela es_ES
dc.contributor.author Rambla Nebot, Jose Luis es_ES
dc.contributor.author Fernández Del Carmen, María Asunción es_ES
dc.contributor.author Trapero Mozos, Almudena es_ES
dc.contributor.author Ahrazem, Oussama es_ES
dc.contributor.author Orzáez Calatayud, Diego Vicente es_ES
dc.contributor.author Granell Richart, Antonio es_ES
dc.contributor.author Gómez Gómez, Lourdes es_ES
dc.date.accessioned 2017-09-20T09:53:51Z
dc.date.available 2017-09-20T09:53:51Z
dc.date.issued 2014-11
dc.identifier.issn 0167-4412
dc.identifier.uri http://hdl.handle.net/10251/87611
dc.description.abstract [EN] Apocarotenoid compounds play diverse communication functions in plants, some of them being as hormones, pigments and volatiles. Apocarotenoids are the result of enzymatic cleavage of carotenoids catalyzed by carotenoid cleavage dioxygenase (CCD). The CCD4 family is the largest family of plant CCDs, only present in flowering plants, suggesting a functional diversification associated to the adaptation for specific physiological capacities unique to them. In saffron, two CCD4 genes have been previously isolated from the stigma tissue and related with the generation of specific volatiles involved in the attraction of pollinators. The aim of this study was to identify additional CCD4 members associated with the generation of other carotenoid-derived volatiles during the development of the stigma. The expression of CsCCD4c appears to be restricted to the stigma tissue in saffron and other Crocus species and was correlated with the generation of megastigma-4,6,8-triene. Further, CsCCD4c was up-regulated by wounding, heat, and osmotic stress, suggesting an involvement of its apocarotenoid products in the adaptation of saffron to environmental stresses. The enzymatic activity of CsCCD4c was determined in vivo in Escherichia coli and subsequently in Nicotiana benthamiana by analyzing carotenoids by HPLC-DAD and the volatile products by GC/MS. beta-Carotene was shown to be the preferred substrate, being cleaved at the 9,10 (9',10') bonds and generating beta-ionone, although beta-cyclocitral resulting from a 7,8 (7',8') cleavage activity was also detected at lower levels. Lutein, neoxanthin and violaxanthin levels in Nicotiana leaves were markedly reduced when CsCCD4c is over expressed, suggesting that CsCCD4c recognizes these carotenoids as substrates. es_ES
dc.description.sponsorship We thank J. Argandona (Instituto Botanico, Universidad de Castilla-La Mancha, Albacete, Spain) for excellent technical support, and K. A. Walsh for language revision. The laboratory is supported by the Spanish Ministerio de Ciencia e Innovacion (BIO2013-44239-R) and participates in the IBERCAROT network (112RT0445). Dr. Ahrazem was funded by FPCYTA through the INCRECYT Programme. en_EN
dc.language Inglés es_ES
dc.publisher Springer Verlag (Germany) es_ES
dc.relation.ispartof Plant Molecular Biology es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Apocarotenoids es_ES
dc.subject Carotenoid cleavage dioxygenases es_ES
dc.subject Saffron es_ES
dc.subject Stigmas es_ES
dc.subject Stress es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s11103-014-0250-5
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//BIO2013-44239-R/ES/ELUCIDACION DE LA BIOSINTESIS, MODIFICACION, ACUMULACION Y REGULACION DE APOCAROTENOIDES EN AZAFRAN Y ESPECIES AFINES MEDIANTE APROXIMACIONES OMICAS/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.description.bibliographicCitation Rubio-Moraga, A.; Rambla Nebot, JL.; Fernández Del Carmen, MA.; Trapero Mozos, A.; Ahrazem, O.; Orzáez Calatayud, DV.; Granell Richart, A.... (2014). New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus. Plant Molecular Biology. 86(4-5):555-569. https://doi.org/10.1007/s11103-014-0250-5 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://doi.org/10.1007/s11103-014-0250-5 es_ES
dc.description.upvformatpinicio 555 es_ES
dc.description.upvformatpfin 569 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 86 es_ES
dc.description.issue 4-5 es_ES
dc.relation.senia 284475 es_ES
dc.identifier.eissn 1573-5028
dc.identifier.pmid 25204497
dc.contributor.funder Ministerio de Economía y Competitividad es_ES
dc.description.references Ahrazem O, Rubio-Moraga A, Lopez RC, Gomez-Gomez L (2010a) The expression of a chromoplast-specific lycopene beta cyclase gene is involved in the high production of saffron’s apocarotenoid precursors. J Exp Bot 61:105–119 es_ES
dc.description.references Ahrazem O, Trapero A, Gomez MD, Rubio-Moraga A, Gomez-Gomez L (2010b) Genomic analysis and gene structure of the plant carotenoid dioxygenase 4 family: a deeper study in Crocus sativus and its allies. Genomics 96:239–250 es_ES
dc.description.references Alder A, Holdermann I, Beyer P, Al-Babili S (2008) Carotenoid oxygenases involved in plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction. Biochem J 416:289–296 es_ES
dc.description.references Arnold K, Bordoli L, Kopp J, Schwede T (2006) The SWISS-MODEL Workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:7 es_ES
dc.description.references Auldridge ME, McCarty DR, Klee HJ (2006) Plant carotenoid cleavage oxygenases and their apocarotenoid products. Curr Opin Plant Biol 9:315–321 es_ES
dc.description.references Baldermann S, Kato M, Kurosawa M, Kurobayashi Y, Fujita A, Fleischmann P, Watanabe N (2010) Functional characterization of a carotenoid cleavage dioxygenase 1 and its relation to the carotenoid accumulation and volatile emission during the floral development of Osmanthus fragrans Lour. J Exp Bot 61:2967–2977 es_ES
dc.description.references Bindon KA, Dry PR, Loveys BR (2007) Influence of plant water status on the production of C13-norisoprenoid precursors in Vitis vinifera L. Cv. cabernet sauvignon grape berries. J Agric Food Chem 55:4493–4500 es_ES
dc.description.references Booker J, Sieberer T, Wright W, Williamson L, Willett B, Stirnberg P, Turnbull C, Srinivasan M, Goddard P, Leyser O (2005) MAX1 encodes a cytochrome P450 family member that acts downstream of MAX3/4 to produce a carotenoid-derived branch-inhibiting hormone. Dev Cell 8:7 es_ES
dc.description.references Bouvier F, Suire C, Mutterer J, Camara B (2003) Oxidative remodeling of chromoplast carotenoids: identification of the carotenoid dioxygenase CsCCD and CsZCD genes involved in Crocus secondary metabolite biogenesis. Plant Cell 15:47–62 es_ES
dc.description.references Brandi F, Bar E, Mourgues F, Horvath G, Turcsi E, Giuliano G, Liverani A, Tartarini S, Lewinsohn E, Rosati C (2011) Study of ‘Redhaven’ peach and its white-fleshed mutant suggests a key role of CCD4 carotenoid dioxygenase in carotenoid and norisoprenoid volatile metabolism. BMC Plant Biol 11:24 es_ES
dc.description.references Brehelin C, Kessler F, van Wijk KJ (2007) Plastoglobules: versatile lipoprotein particles in plastids. Trends Plant Sci 12:260–266 es_ES
dc.description.references Brilli F, Barta C, Fortunati A, Lerdau M, Loreto F, Centritto M (2007) Response of isoprene emission and carbon metabolism to drought in white poplar (Populus alba) saplings. New Phytol 175:244–254 es_ES
dc.description.references Campbell R, Ducreux LJ, Morris WL, Morris JA, Suttle JC, Ramsay G, Bryan GJ, Hedley PE, Taylor MA (2010) The metabolic and developmental roles of carotenoid cleavage dioxygenase4 from potato. Plant Physiol 154:656–664 es_ES
dc.description.references Castillo R, Fernandez JA, Gomez-Gomez L (2005) Implications of carotenoid biosynthetic genes in apocarotenoid formation during the stigma development of Crocus sativus and its closer relatives. Plant Physiol 139:674–689 es_ES
dc.description.references Cunningham FX Jr, Pogson B, Sun Z, McDonald KA, DellaPenna D, Gantt E (1996) Functional analysis of the beta and epsilon lycopene cyclase enzymes of Arabidopsis reveals a mechanism for control of cyclic carotenoid formation. Plant Cell 8:1613–1626 es_ES
dc.description.references Dun EA, Brewer PB, Beveridge CA (2009) Strigolactones: discovery of the elusive shoot branching hormone. Trends Plant Sci 14:364–372 es_ES
dc.description.references Falchi R, Vendramin E, Zanon L, Scalabrin S, Cipriani G, Verde I, Vizzotto G, Morgante M (2013) Three distinct mutational mechanisms acting on a single gene underpin the origin of yellow flesh in peach. Plant J 76:175–187 es_ES
dc.description.references Floss DS, Schliemann W, Schmidt J, Strack D, Walter MH (2008) RNA interference-mediated repression of MtCCD1 in mycorrhizal roots of Medicago truncatula causes accumulation of C27 apocarotenoids, shedding light on the functional role of CCD1. Plant Physiol 148:1267–1282 es_ES
dc.description.references Force A, Lynch M, Pickett FB, Amores A, Yan YL, Postlethwait J (1999) Preservation of duplicate genes by complementary, degenerative mutations. Genetics 151:1531–1545 es_ES
dc.description.references Galpaz N, Ronen G, Khalfa Z, Zamir D, Hirschberg J (2006) A chromoplast-specific carotenoid biosynthesis pathway is revealed by cloning of the tomato white-flower locus. Plant Cell 18:1947–1960 es_ES
dc.description.references Gang DR (2005) Evolution of flavors and scents. Annu Rev Plant Biol 56:301–325 es_ES
dc.description.references Garcia-Limones C, Schnabele K, Blanco-Portales R, Luz Bellido M, Caballero JL, Schwab W, Munoz-Blanco J (2088) Functional characterization of FaCCD1: a carotenoid cleavage dioxygenase from strawberry involved in lutein degradation during fruit ripening. J Agric Food Chem 56:9277–9285 es_ES
dc.description.references Glover BJ (2011) Pollinator attraction: the importance of looking good and smelling nice. Curr Biol 21:R307–R309 es_ES
dc.description.references Ha CV, Leyva-Gonzalez MA, Osakabe Y, Tran UT, Nishiyama R, Watanabe Y, Tanaka M, Seki M, Yamaguchi S, Dong NV, Yamaguchi-Shinozaki K, Shinozaki K, Herrera-Estrella L, Tran LS (2014) Positive regulatory role of strigolactone in plant responses to drought and salt stress. Proc Natl Acad Sci USA 111:851–856 es_ES
dc.description.references Huang FC, Horvath G, Molnar P, Turcsi E, Deli J, Schrader J, Sandmann G, Schmidt H, Schwab W (2009a) Substrate promiscuity of RdCCD1, a carotenoid cleavage oxygenase from Rosa damascena. Phytochemistry 70:457–464 es_ES
dc.description.references Huang FC, Molnar P, Schwab W (2009b) Cloning and functional characterization of carotenoid cleavage dioxygenase 4 genes. J Exp Bot 60:3011–3022 es_ES
dc.description.references Ibdah M, Azulay Y, Portnoy V, Wasserman B, Bar E, Meir A, Burger Y, Hirschberg J, Schaffer AA, Katzir N, Tadmor Y, Lewinsohn E (2006) Functional characterization of CmCCD1, a carotenoid cleavage dioxygenase from melon. Phytochemistry 67:1579–1589 es_ES
dc.description.references Ilg A, Beyer P, Al-Babili S (2009) Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis. FEBS J 276:736–747 es_ES
dc.description.references Ilg A, Yu Q, Schaub P, Beyer P, Al-Babili S (2010) Overexpression of the rice carotenoid cleavage dioxygenase 1 gene in Golden Rice endosperm suggests apocarotenoids as substrates in planta. Planta 232:691–699 es_ES
dc.description.references Kato M, Matsumoto H, Ikoma Y, Okuda H, Yano M (2006) The role of carotenoid cleavage dioxygenases in the regulation of carotenoid profiles during maturation in citrus fruit. J Exp Bot 57:2153–2164 es_ES
dc.description.references Kishimoto S, Sumitomo K, Nakayama M, Ohmiya A (2007) Three routes to orange petal color via carotenoid components in 9 composite species. J Jpn Soc Hortic Sci 76:8 es_ES
dc.description.references Klingner A, Bothe H, Wray V, Marner FJ (1995) Identification of a yellow pigment formed in maize roots upon mycorrhizal colonization. Phytochemistry 38:3 es_ES
dc.description.references Kloer DP, Schulz GE (2006) Structural and biological aspects of carotenoid cleavage. Cell Mol Life Sci 63:2291–2303 es_ES
dc.description.references Lamikanra O, Richard OA, Parker A (2002) Ultraviolet induced stress response in fresh cut cantaloupe. Phytochemistry 60:27–32 es_ES
dc.description.references Lashbrooke JG, Young PR, Dockrall SJ, Vasanth K, Vivier MA (2013) Functional characterisation of three members of the Vitis vinifera L. carotenoid cleavage dioxygenase gene family. BMC Plant Biol 13:156 es_ES
dc.description.references Lewinsohn E, Sitrit Y, Bar E, Azulay Y, Meir A, Zamir D, Tadmor Y (2005) Carotenoid pigmentation affects the volatile composition of tomato and watermelon fruits, as revealed by comparative genetic analyses. J Agric Food Chem 53:3142–3148 es_ES
dc.description.references Lynch M, Conery JS (2003) The origins of genome complexity. Science 302:1401–1404 es_ES
dc.description.references Ma G, Zhang L, Matsuta A, Matsutani K, Yamawaki K, Yahata M, Wahyudi A, Motohashi R, Kato M (2013) Enzymatic formation of beta-citraurin from beta-cryptoxanthin and zeaxanthin by carotenoid cleavage dioxygenase4 in the flavedo of citrus fruit. Plant Physiol 163:682–695 es_ES
dc.description.references Maere S, De Bodt S, Raes J, Casneuf T, Van Montagu M, Kuiper M, Van de Peer Y (2005) Modeling gene and genome duplications in eukaryotes. Proc Natl Acad Sci USA 102:5454–5459 es_ES
dc.description.references Maier W, Peipp H, Schmidt J, Wray V, Strack D (1995) Levels of a terpenoid glycoside (blumenin) and cell wall-bound phenolics in some cereal mycorrhizas. Plant Physiol 109:465–470 es_ES
dc.description.references Mathieu S, Terrier N, Procureur J, Bigey F, Gunata Z (2005) A carotenoid cleavage dioxygenase from Vitis vinifera L.: functional characterization and expression during grape berry development in relation to C13-norisoprenoid accumulation. J Exp Bot 56:2721–2731 es_ES
dc.description.references Messing SA, Gabelli SB, Echeverria I, Vogel JT, Guan JC, Tan BC, Klee HJ, McCarty DR, Amzel LM (2010) Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid. Plant Cell 22:2970–2980 es_ES
dc.description.references Moraga AR, Rambla JL, Ahrazem O, Granell A, Gomez-Gomez L (2009) Metabolite and target transcript analyses during Crocus sativus stigma development. Phytochemistry 70:1009–1016 es_ES
dc.description.references Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S (2007) Improved gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Biosci Biotechnol Biochem 71:2095–2100 es_ES
dc.description.references Nei M, Gojobori T (1986) Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol 3:9 es_ES
dc.description.references Ohmiya A, Kishimoto S, Yoshioka S, Sumitomo K (2005) Cloning of a carotenoid cleavage dioxygenase gene (CmCCD1) differentially expressed in white petals of chrysanthemum. Plant Cell Physiol 46:1 es_ES
dc.description.references Ohmiya A, Kishimoto S, Aida R, Yoshioka S, Sumitomo K (2006) Carotenoid cleavage dioxygenase (CmCCD4a) contributes to white color formation in chrysanthemum petals. Plant Physiol 142:1193–1201 es_ES
dc.description.references Ohmiya A, Sumitomo K, Aida A (2009) ”Yellow Jimba”: supression of carotenoid cleavage dioxygenase (CmCCD4a) expression turns white chrysanthemum petals yellow. J Jpn Soc Hortic Sci 78:6 es_ES
dc.description.references Osborn TC, Pires JC, Birchler JA, Auger DL, Chen ZJ, Lee HS, Comai L, Madlung A, Doerge RW, Colot V, Martienssen RA (2003) Understanding mechanisms of novel gene expression in polyploids. Trends Genet 19:141–147 es_ES
dc.description.references Pfander H, Schurtenberger H (1982) Biosynthesis of C20-Carotenoids in Crocus sativus. Phytochemistry 21:4 es_ES
dc.description.references Rodrigo MJ, Alquézar B, Alós E, Medina V, Carmona L, Bruno M, Al-Babili S, Zacarias L (2013) A novel carotenoid cleavage activity involved in the biosynthesis of Citrus fruit-specific apocarotenoid pigments. J Exp Bot 64(14):4461–4478 es_ES
dc.description.references Ronen G, Carmel-Goren L, Zamir D, Hirschberg J (2000) An alternative pathway to beta-carotene formation in plant chromoplasts discovered by map-based cloning of beta and old-gold color mutations in tomato. Proc Natl Acad Sci USA 97:11102–11107 es_ES
dc.description.references Rosati C, Diretto G, Giuliano G (2010) Biosynthesis and engineering of carotenoids and apocarotenoids in plants: state of the art and future prospects. Biotechnol Genet Eng Rev 26:139–162 es_ES
dc.description.references Rubio A, Rambla JL, Santaella M, Gomez MD, Orzaez D, Granell A, Gomez-Gomez L (2008) Cytosolic and plastoglobule-targeted carotenoid dioxygenases from Crocus sativus are both involved in beta-ionone release. J Biol Chem 283:24816–24825 es_ES
dc.description.references Rubio-Moraga A, Ahrazem O, Perez-Clemente RM, Gomez-Cadenas A, Yoneyama K, Lopez-Raez JA, Molina RV, Gomez-Gomez L (2014) Apical dominance in saffron and the involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting. BMC Plant Biol 14:171 es_ES
dc.description.references Ryle MJ, Hausinger RP (2002) Non-heme iron oxygenases. Curr Opin Chem Biol 6:193–201 es_ES
dc.description.references Sainsbury F, Thuenemann EC, Lomonossoff GP (2009) pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol J 7:682–693 es_ES
dc.description.references Schwartz SH, Tan BC, Gage DA, Zeevaart JA, McCarty DR (1997) Specific oxidative cleavage of carotenoids by VP14 of maize. Science 276:1872–1874 es_ES
dc.description.references Schwartz SH, Qin X, Zeevaart JA (2001) Characterization of a novel carotenoid cleavage dioxygenase from plants. J Biol Chem 276:25208–25211 es_ES
dc.description.references Schwartz SH, Qin X, Loewen MC (2004) The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching. J Biol Chem 279:46940–46945 es_ES
dc.description.references Sharkey TD, Chen X, Yeh S (2001) Isoprene increases thermotolerance of fosmidomycin-fed leaves. Plant Physiol 125:2001–2006 es_ES
dc.description.references Simkin AJ, Schwartz SH, Auldridge M, Taylor MG, Klee HJ (2004a) The tomato carotenoid cleavage dioxygenase 1 genes contribute to the formation of the flavor volatiles beta-ionone, pseudoionone, and geranylacetone. Plant J 40:882–892 es_ES
dc.description.references Simkin AJ, Underwood BA, Auldridge M, Loucas HM, Shibuya K, Schmelz E, Clark DG, Klee HJ (2004b) Circadian regulation of the PhCCD1 carotenoid cleavage dioxygenase controls emission of beta-ionone, a fragrance volatile of petunia flowers. Plant Physiol 136:3504–3514 es_ES
dc.description.references Simkin AJ, Moreau H, Kuntz M, Pagny G, Lin C, Tanksley S, McCarthy J (2008) An investigation of carotenoid biosynthesis in Coffea canephora and Coffea arabica. J Plant Physiol 165:1087–1106 es_ES
dc.description.references Soares VL, Rodrigues SM, de Oliveira TM, de Queiroz TO, Lima LS, Hora-Junior BT, Gramacho KP, Micheli F, Cascardo JC, Otoni WC, Gesteira AS, Costa MG (2011) Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis. Mol Biol Rep 38:1329–1340 es_ES
dc.description.references Sun Z, Hans J, Walter MH, Matusova R, Beekwilder J, Verstappen FW, Ming Z, van Echtelt E, Strack D, Bisseling T, Bouwmeester HJ (2008) Cloning and characterisation of a maize carotenoid cleavage dioxygenase (ZmCCD1) and its involvement in the biosynthesis of apocarotenoids with various roles in mutualistic and parasitic interactions. Planta 228:789–801 es_ES
dc.description.references Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:4 es_ES
dc.description.references Taylor IB, Burbidge A, Thompson AJ (2000) Control of abscisic acid synthesis. J Exp Bot 51:1563–1574 es_ES
dc.description.references Vallabhaneni R, Bradbury LM, Wurtzel ET (2010) The carotenoid dioxygenase gene family in maize, sorghum, and rice. Arch Biochem Biophys 504:104–111 es_ES
dc.description.references Van Norman JM, Zhang J, Cazzonelli CI, Pogson BJ, Harrison PJ, Bugg TD, Chan KX, Thompson AJ, Benfey PN (2014) Periodic root branching in Arabidopsis requires synthesis of an uncharacterized carotenoid derivative. Proc Natl Acad Sci USA 111:E1300–E1309 es_ES
dc.description.references Vickers CE, Gershenzon J, Lerdau MT, Loreto F (2009) A unified mechanism of action for volatile isoprenoids in plant abiotic stress. Nat Chem Biol 5:283–291 es_ES
dc.description.references Vogel JT, Tan BC, McCarty DR, Klee HJ (2008) The carotenoid cleavage dioxygenase 1 enzyme has broad substrate specificity, cleaving multiple carotenoids at two different bond positions. J Biol Chem 283:11364–11373 es_ES
dc.description.references Voinnet O, Rivas S, Mestre P, Baulcombe D (2003) An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 33:949–956 es_ES
dc.description.references Wahlberg I (2002) Carotenoid-derived aroma compounds in tobacco. In: Winterhalter P, Ruseff R (eds) Carotenoid-derived aroma compounds. American Chemical Society, Washington, pp 131–144 es_ES
dc.description.references Walsh JB (1995) How often do duplicated genes evolve new functions? Genetics 139:421–428 es_ES
dc.description.references Walter MH, Strack D (2011) Carotenoids and their cleavage products: biosynthesis and functions. Nat Prod Rep 28(4):663–669 es_ES
dc.description.references Welsch R, Wust F, Bar C, Al-Babili S, Beyer P (2008) A third phytoene synthase is devoted to abiotic stress-induced abscisic acid formation in rice and defines functional diversification of phytoene synthase genes. Plant Physiol 147:367–380 es_ES
dc.description.references Yamamizo C, Kishimoto S, Ohmiya A (2010) Carotenoid composition and carotenogenic gene expression during Ipomoea petal development. J Exp Bot 61:709–719 es_ES
dc.description.references Ytterberg AJ, Peltier JB, van Wijk KJ (2006) Protein profiling of plastoglobules in chloroplasts and chromoplasts. A surprising site for differential accumulation of metabolic enzymes. Plant Physiol 140:984–997 es_ES


Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem