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dc.contributor.author | Vendrell Criado, Victoria | es_ES |
dc.contributor.author | Rodríguez Muñiz, Gemma María | es_ES |
dc.contributor.author | Lhiaubet ., Virginie Lyria | es_ES |
dc.contributor.author | Cuquerella Alabort, Maria Consuelo | es_ES |
dc.contributor.author | Miranda Alonso, Miguel Ángel | es_ES |
dc.date.accessioned | 2018-02-18T05:02:40Z | |
dc.date.available | 2018-02-18T05:02:40Z | |
dc.date.issued | 2016 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/98042 | |
dc.description.abstract | [EN] Based on our previous investigations into the photophysical properties of the 5-methyl-2-pyrimidone (Pyo) chromophore, we now extend our studies to the photobehavior of the dimeric (6-4) thymine photoproducts (6-4 PP) to evaluate their capability to act as instrinsic DNA photosensitizers. The lesion presents significant absorption in the UVB/UVA region, weak fluorescence emission, a singlet-excited-state energy of approximately 351 kJ mol(-1), and a triplet-excited-state energy of 297 kJ mol(-1). Its triplet transient absorption has a maximum at 420-440 nm, a lifetime of around 7 mu s, and a high formation quantum yield, Phi(ISC) = 0.86. This species is efficiently quenched by thymidine. Its DNA photosensitizing properties are demonstrated by a series of experiments run on a pBR322 plasmid. The lesion photoinduces both single-strand breaks and the formation of cyclobutane thymine dimers. Altogether, these results show that, the substitution of the pyrimidone ring at C4 by a 5-hydroxy-5,6-dihydrothymine does not cancel out the photosensitization properties of the chromophore. | es_ES |
dc.description.sponsorship | Spanish Government (CTQ2015-70164-P, RIRAAF RETICS RD12/0013/0009, Prometeo II program, Severo Ochoa program/SEV-2012-0267 and JAE-Predoc 2011-00740 to V. V.-C.) is gratefully acknowledged. | |
dc.language | Inglés | es_ES |
dc.publisher | John Wiley & Sons | es_ES |
dc.relation.ispartof | ChemPhysChem (Online) | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject | Cyclobutane pyrimidine dimers | es_ES |
dc.subject | DNA damage | es_ES |
dc.subject | Photochemistry | es_ES |
dc.subject | Pyrimidone | es_ES |
dc.subject | Triplet excited states | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.title | The (6-4) Dimeric Lesion as a DNA Photosensitizer | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1002/cphc.201600154 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//SEV-2012-0267/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//RD12%2F0013%2F0009/ES/Reacciones adversas a alérgenos y fármacos/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO//CTQ2015-70164-P/ES/LESIONES DEL ADN COMO FOTOSENSIBILIZADORES INTRINSECOS - CONCEPTO DE CABALLO DE TROYA/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Instituto Universitario Mixto de Tecnología Química - Institut Universitari Mixt de Tecnologia Química | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Vendrell Criado, V.; Rodríguez Muñiz, GM.; Lhiaubet ., VL.; Cuquerella Alabort, MC.; Miranda Alonso, MÁ. (2016). The (6-4) Dimeric Lesion as a DNA Photosensitizer. ChemPhysChem (Online). 17(13):1979-1982. https://doi.org/10.1002/cphc.201600154 | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1002/cphc.201600154 | es_ES |
dc.description.upvformatpinicio | 1979 | es_ES |
dc.description.upvformatpfin | 1982 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 17 | es_ES |
dc.description.issue | 13 | es_ES |
dc.identifier.eissn | 1439-7641 | es_ES |
dc.identifier.pmid | 26990589 | |
dc.relation.pasarela | S\318609 | es_ES |
dc.contributor.funder | Ministerio de Economía y Competitividad | es_ES |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |