Zhai, Jixian; Bischof, Sylvain; Wang, Haifeng; Feng, Suhua; Lee, Tzuu-Fen; Teng, Chong; Chen, Xinyuan; Park, Soo Young; Liu, Linshan; Gallego Bartolomé, Javier; Liu, Wanlu; Henderson, Ian R; Meyers, Blake C.; Ausin, Israel; Jacobsen, Steven E.(Elsevier, 2015-10-08)
[EN] RNA-directed DNA methylation in Arabidopsis thaliana is driven by the plant-specific RNA Polymerase IV (Pol IV). It has been assumed that a Pol IV transcript can give rise to multiple 24-nt small interfering RNAs ...
Gallego Bartolomé, Javier; Liu, Wanlu; Kuo, Peggy Hsuanyu; Feng, Suhua; Ghoshal, Basudev; Gardiner, Jason; Zhao, Jenny Miao-Chi; Park, Soo Young; Chory, Joanne; Jacobsen, Steven E.(Elsevier, 2019-02-21)
[EN]
The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation ...
[EN] Over millions of years, eukaryotes evolved from unicellular to multicellular organisms with increasingly complex genomes and sophisticated gene expression networks. Consequently, chromatin regulators evolved to support ...
Wongpalee, Somsakul Pop; Liu, Shiheng; Gallego Bartolomé, Javier; Leitner, Alexander; Aebersold, Ruedi; Liu, Wanlu; Yen, Linda; Nohales, Maria A.; Kuo, Peggy Hsuanyu; Vashisht, Ajay A.; Wohlschlegel, James A.; Feng, Suhua; Kay, Steve A.; Zhou, Z. Hong; Jacobsen, Steven E.(Nature Publishing Group, 2019-09-02)
[EN]
Transcription by RNA polymerase V (Pol V) in plants is required for RNA-directed DNA methylation, leading to transcriptional gene silencing. Global chromatin association of Pol V requires components of the DDR complex ...
Ausin, Israel; Feng, Suhua; Yu, Chaowei; Liu, Wanlu; Kuo, Hsuan Yu; Jacobsen, Elise L.; Zhai, Jixian; Gallego Bartolomé, Javier; Wang, Lin; Egertsdotter, Ulrika; Street, Nathaniel R.; Jacobsen, Steven E.; Wang, Haifeng(Proceedings of the National Academy of Sciences, 2016-12-13)
[EN] DNA methylation plays important roles in many biological processes, such as silencing of transposable elements, imprinting, and regulating gene expression. Many studies of DNA methylation have shown its essential roles ...
[EN] The ability to target epigenetic marks like DNA methylation to specific loci is important in both basic research and in crop plant engineering. However, heritability of targeted DNA methylation, how it impacts gene ...
Hale, Christopher J.; Potok, Magdalena E.; Lopez, Jennifer; Do, Truman; Liu, Ao; Gallego Bartolomé, Javier; Michaels, Scott D.; Jacobsen, Steven E.(Public Library of Science, 2016-06)
[EN]
Eukaryotic genomes are regulated by epigenetic marks that act to modulate transcriptional control as well as to regulate DNA replication and repair. In Arabidopsis thaliana, mutation of the ATXR5 and ATXR6 histone ...
Zhong, Xuehua; Du, Jiamu; Hale, Christopher J; Gallego Bartolomé, Javier; Feng, Suhua; Vashisht, Ajay A.; Chory, Joanne; Wohlschlegel, James A.; Patel, Dinshaw J.; Jacobsen, Steven E.(Elsevier, 2014-05-26)
[EN]
DNA methylation is a conserved epigenetic generegulation mechanism. DOMAINS REARRANGED METHYLTRANSFERASE (DRM) is a key de novo methyltransferase in plants, but how DRM acts mechanistically is poorly understood. ...
[EN] Small RNAs regulate chromatin modifications such as DNA methylation and gene silencing across eukaryotic genomes. In plants, RNA-directed DNA methylation (RdDM) requires 24-nucleotide small interfering RNAs (siRNAs) ...
Gallego Bartolomé, Javier; Gardiner, Jason; Liu, Wanlu; Papikian, Ashot; Ghoshal, Basudev; Kuo, Hsuan Yu; Zhao, Jenny Miao-Chi; Segal, David J.; Jacobsen, Steven E.(Proceedings of the National Academy of Sciences, 2018-02-27)
[EN] DNA methylation is an important epigenetic modification involved in gene regulation and transposable element silencing. Changes in DNA methylation can be heritable and, thus, can lead to the formation of stable ...