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Browsing by Author "Fernández Del Carmen, María Asunción"

Now showing items 1-13 of 13

A coat-independent superinfection exclusion rapidly imposed in Nicotiana benthamiana cells by tobacco mosaic virus is not prevented by depletion of the movement protein

Julve Parreño, Jose Manuel; Gandia Fernàndez, Antoni; Fernández Del Carmen, María Asunción; Sarrion-Perdigones, Alejandro; Castelijns, Bas; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer Verlag (Germany), 2013-04)

[EN] New evidence is emerging which indicates that population variants in plant virus infections are not uniformly distributed along the plant, but structured in a mosaic-like pattern due to limitation to the superinfection ...
A modular toolbox for gRNA-Cas9 genome engineering in plants based on the GoldenBraid standard

Vázquez-Vilar, Marta; Bernabé-Orts, Joan Miquel; Fernández Del Carmen, María Asunción; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Springer (Biomed Central Ltd.), 2016)

[EN] Background: The efficiency, versatility and multiplexing capacity of RNA-guided genome engineering using the CRISPR/Cas9 technology enables a variety of applications in plants, ranging from gene editing to the ...
Evaluation of unintended effects in the composition of tomatoes expressing a human immunoglobulin A against Rotavirus

Juárez Ortega, Paloma; Fernández Del Carmen, María Asunción; Rambla Nebot, Jose Luis; Presa Castro, Silvia; Mico, Amparo; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (American Chemical Society, 2014-08-13)

The production of neutralizing immunoglobulin A (IgA) in edible fruits as a means of oral passive immunization is a promising strategy for the inexpensive treatment of mucosal diseases. This approach is based on the ...
Fine-tuning tomato agronomic properties by computational genome redesign

Carrera Montesinos, Javier; Fernández Del Carmen, María Asunción; Fernández Muñoz, Rafael; Rambla Nebot, Jose Luis; Pons Puig, Clara; Jaramillo Rosales, Alfonso; Elena Fito, Santiago Fco; Granell Richart, Antonio (Public Library of Science, 2012-06-07)

[EN] Considering cells as biofactories, we aimed to optimize its internal processes by using the same engineering principles that large industries are implementing nowadays: lean manufacturing. We have applied reverse ...
GB3.0: a platform for plant bio-design that connects functional DNA elements with associated biological data

Vázquez-Vilar, Marta; Quijano-Rubio, Alfredo; Fernández Del Carmen, María Asunción; Sarrion-Perdigones, Alejandro; Ochoa-Fernández, Rocío; Ziarsolo Areitioaurtena, Pello; Blanca Postigo, José Miguel; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Oxford University Press, 2017)

[EN] Modular DNA assembly simplifies multigene engineering in Plant Synthetic Biology. Furthermore, the recent adoption of a common syntax to facilitate the exchange of plant DNA parts (phytobricks) is a promising strategy ...
GoldenBraid: an iterative simple standardized cloning system system for standardized assembly assembly of recyclable reusable genetic modules

Sarrión Perdigones, Manuel Alejandro; Juárez Ortega, Paloma; Fernández Del Carmen, María Asunción; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Public Library of Science, 2011)

Synthetic Biology requires efficient and versatile DNA assembly systems to facilitate the building of new genetic modules/pathways from basic DNA parts in a standardized way. Here we present GoldenBraid (GB), a standardized ...
Highly efficient genomics-assisted development of a library of introgression lines of Solanum pimpinellifolium

Barrantes, Walter; Fernández Del Carmen, María Asunción; Lopez-Casado, Gloria; González-Sánchez, María Ángeles; Fernandez-Munoz, Rafael; Granell Richart, Antonio; Monforte Gilabert, Antonio José (Springer Verlag (Germany), 2014-12)

The Solanum pimpinellifolium L. accession TO-937 is resistant to pests due to the presence of type IV glandular trichomes and also has the potential to increase fruit quality traits in tomato cultivars. This accession was ...
Identification, introgression, and validation of fruit volatile QTLs from a red-fruited wild tomato species

Rambla Nebot, Jose Luis; Medina, Aurora; Fernández Del Carmen, María Asunción; Barrantes, Walter; Grandillo, Silvana; Cammareri, Maria; López Casado, Gloria; Rodrigo Tarrega, Guillermo; Alonso, Arancha; Garcia-Martinez, S; Primo Millo, Jaime; Ruiz, JJ; Fernandez-Muñoz, R; Monforte Gilabert, Antonio José; Granell Richart, Antonio (Oxford University Press (OUP), 2016-01)

[EN] Volatile organic compounds (VOCs) are major determinants of fruit flavor, a primary objective in tomato breeding. A recombinant inbred line (RIL) population consisting of 169 lines derived from a cross between Solanum ...
New target carotenoids for CCD4 enzymes are revealed with the characterization of a novel stress-induced carotenoid cleavage dioxygenase gene from Crocus sativus

Rubio-Moraga, Angela; Rambla Nebot, Jose Luis; Fernández Del Carmen, María Asunción; Trapero Mozos, Almudena; Ahrazem, Oussama; Orzáez Calatayud, Diego Vicente; Granell Richart, Antonio; Gómez Gómez, Lourdes (Springer Verlag (Germany), 2014-11)

[EN] Apocarotenoid compounds play diverse communication functions in plants, some of them being as hormones, pigments and volatiles. Apocarotenoids are the result of enzymatic cleavage of carotenoids catalyzed by carotenoid ...
Recombinant jacalin-like plant lectins are produced at high levels in Nicotiana benthamiana and retain agglutination activity and sugar specificity

Fernández Del Carmen, María Asunción; Juárez Ortega, Paloma; Presa Castro, Silvia; Granell Richart, Antonio; Orzáez Calatayud, Diego Vicente (Elsevier, 2013-02-20)

The plant kingdom is an underexplored source of valuable proteins which, like plant lectins, display unique interacting specificities. Furthermore, plant protein diversity remains under-exploited due to the low availability ...
Strong and tunable anti-CRISPR/Cas activities in plants

Calvache-Román, Camilo Alberto; Selma, Sara; Vázquez-Vilar, Marta; Uranga, Mireia; Fernández-Del-Carmen, Asun; Daròs, José-Antonio; Orzáez Calatayud, Diego Vicente (Blackwell Publishing, 2022-02)

[EN] CRISPR/Cas has revolutionized genome engineering in plants. However, the use of anti-CRISPR proteins as tools to prevent CRISPR/Cas-mediated gene editing and gene activation in plants has not been explored yet. This ...
The tomato genome sequence provides insights into fleshy fruit evolution

Sato, Shusei; Tabata, Satoshi; Hirakawa, Hideki; Asamizu, Erika; Shirasawa, Kenta; Isobe, Sachiko; Kaneko, Takakazu; Nakamura, Yasukazu; Shibata, Daisuke; Aoki, Koh; Egholm, Michael; Fernández Del Carmen, María Asunción; Monforte Gilabert, Antonio José; Granell Richart, Antonio; Fernandez-Munoz, Rafael (MOST/2006AA10A116, 2012-05-31)

[EN] Tomato (Solanum lycopersicum) is a major crop plant and a model system for fruit development. Solanum is one of the largest angiosperm genera(1) and includes annual and perennial plants from diverse habitats. Here we ...
Wide-genome QTL mapping of fruit quality traits in a tomato RIL population derived from the wild-relative species Solanum pimpinellifolium L

Capel, C; Fernández Del Carmen, María Asunción; Alba, JM; Lima-Silva, V; Hernandez-Gras, F; Salinas, M; Boronat, A; Angosto, T; Botella, MA; Fernández Muñoz, Rafael; Granell Richart, Antonio; Capel, J; Lozano, R; Capel (Springer Verlag (Germany), 2015-10)

[EN] QTL and candidate genes associated to fruit quality traits have been identified in a tomato genetic map derived from Solanum pimpinellifolium L., providing molecular tools for marker-assisted breeding. The study ...