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Browsing by Subject "Microspore culture"

Now showing items 1-9 of 9

Androgenesis in recalcitrant solanaceous crops

Seguí-Simarro, Jose M.; Corral Martínez, Patricia; Parra Vega, Verónica; Gonzalez Garcia, Beatriz (Springer Verlag (Germany), 2011-05)

[EN] Tomato, eggplant, and pepper are three solanaceous crops of outstanding importance worldwide. For hybrid seed production in these species, a fast and cheap method to obtain pure (homozygous) lines is a priority. ...
Calcium and cell wall dynamics during microspore embryogenesis and double haploid production in rapeseed and eggplant

Rivas Sendra, Alba (Universitat Politècnica de València, 2017-07-20)

Androgenesis induction is an experimental procedure by which microspores are diverted from their original gametophytic pathway towards embryogenesis by applying specific stresses in vitro. It allows for the production of ...
Comparison of six different methods to calculate cell densities

Camacho-Fernández, Carolina; Hervás, David; Rivas-Sendra, Alba; Marín, Mª Pilar; Seguí-Simarro, Jose M. (Springer (Biomed Central Ltd.), 2018)

[EN] Background: For in vitro culture of plant and animal cells, one of the critical steps is to adjust the initial cell density. A typical example of this is isolated microspore culture, where specific cell densities ...
Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response

Rivas-Sendra, Alba; Manuel Campos-Vega; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Springer-Verlag, 2017)

[EN] We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. ...
Doubled haploids in eggplant

Mir Moreno, Ricardo; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (MDPI AG, 2021-07)

[EN] This review compiles the most relevant advances made in the production of doubled haploid plants in eggplant, the application of doubled haploid lines in breeding programs, and the future perspectives for the development ...
Dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in Brassica napus and Solanum melongena

Rivas-Sendra, Alba; Calabuig-Serna, Antonio; Seguí-Simarro, Jose M. (Frontiers Media SA, 2017)

[EN] Calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. However, there are no direct clues about calcium levels ...
Effects of growth conditions of donor plants and in vitro culture environment in the viability and the embryogenic response of microspores of different eggplant genotypes

Rivas-Sendra, Alba; Corral Martínez, Patricia; Camacho-Fernández, Carolina; Porcel, R; Seguí-Simarro, Jose M. (Springer-Verlag, 2020-10-06)

[EN] Notwithstanding the importance of eggplant in global horticulture, doubled haploid production in this species is still far from being efficient. Although acknowledged to have a role in the efficiency of androgenesis ...
Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis

Rivas Sendra, Alba; Corral Martínez, Patricia; Camacho Fernández, Carolina; Seguí-Simarro, Jose M. (Springer Verlag (Germany), 2015-09)

[EN] Doubled haploid (DH) technology allows for the production of pure lines, useful for plant breeding, through a one-generation procedure that reduces considerably the time and resources needed to produce them. ...
Microspore embryogenesis: cell wall dynamics and reprogramming of cell fate

Camacho Fernández, Carolina (Universitat Politècnica de València, 2021-03-08)

[ES] Los dobles haploides son una gran herramienta para la mejora genética de híbridos debido a que se puede alcanzar la homocigosis completa en una sola generación. Entre las técnicas usadas para obtener estas plantas, ...