(Springer New York, 2016) Antoni-Alandes, Regina; Dietrich, Daniela; Bennett, Malcolm J.; Rodríguez Egea, Pedro Luís; Instituto Universitario Mixto de Biología Molecular y Celular de Plantas; Duque, Paula
[EN] Hydrotropism is a genuine response of roots to a moisture gradient to avoid drought. An experimental
system for the induction of hydrotropic root response in petri dishes was designed by pioneering groups
in the fi eld. This system uses split agar plates containing an osmolyte only in a region of the plate in order
to generate a water potential gradient. Arabidopsis seedlings are placed on the MS agar plate so that their
root tips are near the junction between plain MS medium and the region supplemented with the osmolyte.
This elicits a hydrotropic response in Arabidopsis roots that can be measured as the root curvature angle.
