Sierra Robles, Santiago

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Author: Fita Fernández, Ana María ×

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    First Ex Situ In Vitro Propagation Protocol of Coronilla viminalis Salisb., An Endangered Fodder Species Adapted to Drought and Salinity
    (MDPI AG, 2024-03) Sierra Robles, Santiago; Cortés Olmos, Carles; Pallotti Sagripanti, Claudia Gabriela; Rodríguez Burruezo, Adrián; Pineda Chaza, Benito José; Fita Fernández, Ana María; Departamento de Producción Vegetal; Instituto Universitario Mixto de Biología Molecular y Celular de Plantas; Departamento de Biotecnología; Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana; Escuela Politécnica Superior de Gandia; Escuela Técnica Superior de Ingeniería Agronómica y del Medio Natural
    [EN] Coronilla viminalis Salisb. is a fodder leguminous plant from the Canary Islands and Northwestern Africa with adaptation to drought. Its conservation status is critical. Its low germination capacity and intense loss of viability of the seeds over time complicate its conservation, limiting its long-term maintenance in germplasm banks and hampering its potential use as a resilient fodder crop. Therefore, in this work, two alternative propagation methods have been addressed. The first was based on facilitating seed germination under aseptic conditions, supplementing the media with gibberellic acid (GA(3)). The latter consisted of creating a micropropagation method from nodal segments and testing different media and pretreatments with indole-3-acetic acid (IAA). The quantity and quality of the roots were assessed, and the plant acclimation rate was measured. The results showed that the optimum concentration of GA(3) for germination, up to 98%, was 250 PPM. The experiments showed that using IAA in the micropropagation media is critical for in vitro rooting in this species. A hormonal pretreatment with IAA significantly improved the rooting efficiency compared to supplementing it into the culture media. One hundred percent of acclimated plants survived the process. These new protocols will help conserve the species and explore its possibilities as fodder crops.