Effective generation of genetically modified rabbits by sperm mediated gene transfer
| dc.contributor.author | Vasicek, D. | |
| dc.contributor.author | Vasickova, K. | |
| dc.contributor.author | Parkanyi, V. | |
| dc.contributor.author | Rafay, J. | |
| dc.contributor.author | Ondruska, L. | |
| dc.contributor.funder | Slovak Research and Development Agency | |
| dc.date.accessioned | 2011-02-08T12:07:15Z | |
| dc.date.available | 2011-02-08T12:07:15Z | |
| dc.date.issued | 2007 | |
| dc.date.updated | 2011-02-08T11:13:25Z | |
| dc.description.abstract | [EN] Many reports in the past decade showing successful sperm mediated gene transfer (SMGT) offoreign DNA into both non-mammalian and mammalian animals. Fresh collected, extender washed rabbit spermcells were co-cultured with linear 14.3 kb gene construct and used for AI. Transgene integration in rabbitgenome was verified by PCR analysis. The PCR results show that transgenic founder rabbits transmittedtransgene to F1 generation. Our preliminary results suggest that SMGT could be an effective method forproduction of genetically modified rabbits. | en_EN |
| dc.description.accrualMethod | SWORD | es_ES |
| dc.description.bibliographicCitation | Vasicek, D.; Vasickova, K.; Parkanyi, V.; Rafay, J.; Ondruska, L. (2007). Effective generation of genetically modified rabbits by sperm mediated gene transfer. World Rabbit Science. 15(3). https://doi.org/10.4995/wrs.2007.593 | es_ES |
| dc.description.issue | 3 | |
| dc.description.sponsorship | We would like to thank Mr. Jan Pecho and Dipl. Ing. Ivan Chlebec for taking care of the animals and for providing technical assistance. This work was financially supported by Ministry of Agriculture Slovak Republic and Slovak Research and Development Agency under the contract No. APVV-27-005505. | |
| dc.description.volume | 15 | |
| dc.identifier.doi | 10.4995/wrs.2007.593 | |
| dc.identifier.eissn | 1989-8886 | es_ES |
| dc.identifier.issn | 1257-5011 | |
| dc.identifier.uri | https://riunet.upv.es/handle/10251/9486 | |
| dc.language | Inglés | es_ES |
| dc.publisher | World Rabbit Science. ICTA. UPV | es_ES |
| dc.relation.ispartof | World Rabbit Science | |
| dc.relation.projectID | info:eu-repo/grantAgreement/SRDA//APVV-27-005505/ | |
| dc.relation.publisherversion | https://doi.org/10.4995/wrs.2007.593 | |
| dc.rights | Reserva de todos los derechos | es_ES |
| dc.rights.accessRights | Abierto | es_ES |
| dc.subject | Transgenic rabbit | es_ES |
| dc.subject | Sperm mediated gene transfer (smgt) | es_ES |
| dc.subject | Human factor viii | es_ES |
| dc.title | Effective generation of genetically modified rabbits by sperm mediated gene transfer | es_ES |
| dc.type | Artículo | es_ES |
| dspace.entity.type | Publication | |
| upv.uuid | ff486821-8e0f-4e8b-a2f4-97fa325a199b | es_ES |
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