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Induction of cinnamate 4-hydroxylase and phenylpropanoids in virus-infected cucumber and melon plants

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Induction of cinnamate 4-hydroxylase and phenylpropanoids in virus-infected cucumber and melon plants

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dc.contributor.author Belles Albert, José Mª es_ES
dc.contributor.author López-Gresa, María Pilar es_ES
dc.contributor.author Fayos, J. es_ES
dc.contributor.author Pallás Benet, Vicente es_ES
dc.contributor.author Rodrigo Bravo, Ismael es_ES
dc.contributor.author Conejero Tomás, Vicente es_ES
dc.date.accessioned 2018-04-13T04:14:33Z
dc.date.available 2018-04-13T04:14:33Z
dc.date.issued 2008 es_ES
dc.identifier.issn 0168-9452 es_ES
dc.identifier.uri http://hdl.handle.net/10251/100331
dc.description.abstract [EN] In the present work, we have looked for the nature of the phenylpropanoids biosynthesized during the plant-pathogen reaction of two systems, Cucumis sativus and Cucumis melo infected with either prunus necrotic ringspot virus (PNRSV) or melon necrotic spot virus (MNSV), respectively. An accumulation of p-coumaric, caffeic and/or ferulic acids was observed in infected plant extracts hydrolysed with P-glucosidase or esterase. Analysis of undigested samples by HPLC/ESI revealed that these compounds are mainly forming esters with glucose: 1-O-coumaroyl-beta-glucose, 1-O-caffeoyl-beta-glucose, and 1-O-fer-uloyl-beta-glucose. Cinnamic acid 4-hydroxylase (C4H, EC 1.14.13.11), the second enzyme of the plant phenylpropanoid pathway, plays a pivotal role in the synthesis of these hydroxycinnamic acids. Thus, we have isolated and characterised a cDNA clone encoding this enzyme from PNRSV-infected cucumber, and a partial cDNA from MNSV-infected melon leaves. The deduced amino acid sequence revealed a notable degree of identity with homologous C4H enzymes from other plant species. In agreement with the induction of the phenylpropanoids presently described, it is reported that in cucumber and melon leaves, both viral infections studied induced C4H mRNA expression. A similar induction was observed for the first pherylpropanoid biosynthetic enzyme, phyenylalanine ammonia-lyase (PAL, EC 4.3.1.5), and for chitinase and peroxidase defence-related genes. es_ES
dc.description.sponsorship We gratefully acknowledge Dr. Lynne Yenush for critical reading of the manuscript and helpful discussions, and L. Latorre, and L. Coracha´n for the technical support in the greenhouse. The authors want to acknowledge the detailed suggestions of the referees. This work was supported by Grant BMC2000-1136 from Comisio´n Interministerial de Ciencia y Tecnologı´a, Spanish Ministry of Science and Technology
dc.language Inglés es_ES
dc.publisher Elsevier es_ES
dc.relation DGCS/BMC2000-1136 es_ES
dc.relation.ispartof Plant Science es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Cucumis sativus es_ES
dc.subject Cucumis melo es_ES
dc.subject Cinnamic acid 4-hydroxylase es_ES
dc.subject Compatible interactions es_ES
dc.subject Phenylpropanoids es_ES
dc.subject.classification BIOQUIMICA Y BIOLOGIA MOLECULAR es_ES
dc.title Induction of cinnamate 4-hydroxylase and phenylpropanoids in virus-infected cucumber and melon plants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1016/j.plantsci.2008.02.008 es_ES
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1016/j.plantsci.2008.02.008 es_ES
dc.description.upvformatpinicio 524 es_ES
dc.description.upvformatpfin 533 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 174 es_ES
dc.relation.pasarela S\34489 es_ES
dc.contributor.funder Comisión Interministerial de Ciencia y Tecnología es_ES


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