Martínez Turiño, S.; Hernandez Fort, C. (2011). A membrane-associated movement protein of Pelargonium flower break virus shows
RNA-binding activity and contains a biologically relevant leucine zipper-like motif. Virology. 413(2):310-319. doi:10.1016/j.virol.2011.03.001
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/100988
Title:
|
A membrane-associated movement protein of Pelargonium flower break virus shows
RNA-binding activity and contains a biologically relevant leucine zipper-like motif
|
Author:
|
Martínez Turiño, Sandra
Hernandez Fort, Carmen
|
UPV Unit:
|
Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes
|
Issued date:
|
|
Abstract:
|
[EN] Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each ...[+]
[EN] Two small viral proteins (DGBp1 and DGBp2) have been proposed to act in a concerted manner to aid intra- and intercellular trafficking of carmoviruses though the distribution of functions and mode of action of each protein partner are not yet clear. Here we have confirmed the requirement of the DGBps of Pelargonium flower break virus (PFBV), p7 and p12, for pathogen movement Studies focused on p12 have shown that it associates to cellular membranes, which is in accordance to its hydrophobic profile and to that reported for several homologs. However, peculiarities that distinguish p12 from other DGBp52 have been found. Firstly, it contains a leucine zipper-like motif which is essential for virus infectivity in plants. Secondly, it has an unusually long and basic N-terminal region that confers RNA binding activity. The results suggest that PFBV p12 may differ mechanistically from related proteins and possible roles of PFBV DGBps are discussed. (C) 2011 Elsevier Inc. All rights reserved.
[-]
|
Subjects:
|
Carmovirus
,
PFBV
,
Movement proteins
,
RNA-binding domain
,
Leucine zipper-like motif
,
Membrane association
,
Subcellular localization
|
Copyrigths:
|
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
|
Source:
|
Virology. (issn:
0042-6822
)
|
DOI:
|
10.1016/j.virol.2011.03.001
|
Publisher:
|
Elsevier
|
Publisher version:
|
http://doi.org/10.1016/j.virol.2011.03.001
|
Project ID:
|
MICINN/BFU2009-11699
MEC/BFU2006-11230
GV/ACOM06/210
Consellería d'Educació de la GV/ACOMP/2009/040
|
Thanks:
|
We gratefully thank Dr. Vicente Pallas for critical reading of the manuscript and Dolores Arocas and Isabella Avellaneda for their technical assistance. This research was supported by grant BFU2006-11230 and BFU2009-11699 ...[+]
We gratefully thank Dr. Vicente Pallas for critical reading of the manuscript and Dolores Arocas and Isabella Avellaneda for their technical assistance. This research was supported by grant BFU2006-11230 and BFU2009-11699 from the Ministerio Ciencia e Innovacion (MICINN, Spain) and by grants ACOM/2006/210 and ACOMP/2009/040 (Generalitat Valenciana, GV) to C. H. S. M.-T. was the recipient of a predoctoral fellowship from GV and of a predoctoral contract from MICINN.
[-]
|
Type:
|
Artículo
|