Zacarés Sanmartín, L.; López-Gresa, MP.; Fayos Febrer, J.; Primo Millo, J.; Belles Albert, JM.; Conejero Tomás, V. (2007). Induction of p-coumaroyidopamine and feruloyidopamine, two novel metabolites, in tomato by the bacterial pathogen Pseudomonas syringae. Molecular Plant-Microbe Interactions. 20(11):1439-1448. doi:10.1094 / MPMI -20-11-1439
Por favor, use este identificador para citar o enlazar este ítem: http://hdl.handle.net/10251/101128
Title:
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Induction of p-coumaroyidopamine and feruloyidopamine, two novel metabolites, in tomato by the bacterial pathogen Pseudomonas syringae
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Author:
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Zacarés Sanmartín, Laura
López-Gresa, María Pilar
Fayos Febrer, Joaquín
Primo Millo, Jaime
Belles Albert, José Mª
Conejero Tomás, Vicente
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UPV Unit:
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Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia
Universitat Politècnica de València. Departamento de Química - Departament de Química
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Issued date:
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Abstract:
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[EN] Inoculation of tomato plants (Solanum lycopersicum cv. Rutgers) with Pseudomonas syringae pv. tomato led to the production of a hypersensitive-like response in this pathovar of tomato. Accumulation of hydroxycinnamic ...[+]
[EN] Inoculation of tomato plants (Solanum lycopersicum cv. Rutgers) with Pseudomonas syringae pv. tomato led to the production of a hypersensitive-like response in this pathovar of tomato. Accumulation of hydroxycinnamic acid amides (HCAA) of tyramine (p-coumaroyltyramine and feruloyltyramine) and dopamine (p-coumaroyldopamine and feruloyldopamine) was detected after bacterial infection. Two of them, p-coumaroyldopamine and feruloyldopamine, are described for the first time. The accumulation of HCAA was preceded by an increment of hydroxycinnamoyl-CoA:tyramine N-hydroxycinnamoyl transferase (THT) gene expression. HCAA also accumulated in transgenic NahG tomato plants overexpressing a bacterial salicylic hydroxylase. However, treatment of plants with the ethylene biosynthesis inhibitor, aminoethoxyvinilglycine, led to a reduction in the accumulation of THT transcripts and HCAA. Together, the results suggest that pathogen-induced induction of ethylene is essential for HCAA synthesis, whereas salicylic acid is not required for this response. In addition, notable antibacterial and antioxidant activities were found for the new HCAA, thus indicating that they could play a role in the defense of tomato plants against bacterial infection.
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Copyrigths:
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Reserva de todos los derechos
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Source:
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Molecular Plant-Microbe Interactions. (issn:
0894-0282
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DOI:
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10.1094 / MPMI -20-11-1439
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Publisher:
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Scientific Societies
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Publisher version:
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http://doi.org/10.1094 / MPMI -20-11-1439
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Project ID:
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info:eu-repo/grantAgreement/MICYT//BMC2000-1136/
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Thanks:
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This work was supported by Grant BMC2000-1136 from Comisión Interministerial de Ciencia y Tecnología, Spanish Ministry of Science and Technology. L. Zacarés was supported by a fellowship CTBPRB/2003/112 from Generalitat ...[+]
This work was supported by Grant BMC2000-1136 from Comisión Interministerial de Ciencia y Tecnología, Spanish Ministry of Science and Technology. L. Zacarés was supported by a fellowship CTBPRB/2003/112 from Generalitat Valenciana, Conselleria de Empresa, Universidad y Ciencia, Spain. We gratefully acknowledge J. D. G. Jones (John Innes Centre, Norwich, U.K.) for the gift of seed of the transgenic NahG tomato plants and L. Yenush for critical reading of the manuscript and helpful discussions. I. Rodrigo is acknowledged for his continuous support during the course of this project.
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Type:
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Artículo
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