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dc.contributor.author | Vayá Pérez, Ignacio | es_ES |
dc.contributor.author | Jiménez Molero, María Consuelo | es_ES |
dc.contributor.author | Miranda Alonso, Miguel Ángel | es_ES |
dc.date.accessioned | 2018-05-11T04:19:52Z | |
dc.date.available | 2018-05-11T04:19:52Z | |
dc.date.issued | 2008 | es_ES |
dc.identifier.issn | 1520-6106 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10251/101716 | |
dc.description | This document is the Accepted Manuscript version of a Published Work that appeared in final form in The Journal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://doi.org/10.1021/jp076960q | |
dc.description.abstract | [EN] Laser flash photolysis (LFP) has been used to determine the degree of binding of (S)- or (R)-flurbiprofen methyl ester (FBPMe) to human and bovine serum albumins (HSA and BSA, respectively). Regression analysis of the triplet decay of the drug (lambda = 360 nm) in the presence of the proteins led to a satisfactory fitting when considering a set of three lifetimes; the corresponding A(free), A(I) and A(II) preexponential coefficients can be correlated with the presence of FBPMe in the bulk solution and within the two known binding sites. The most remarkable differences between HSA and BSA were found under nonsaturating conditions; thus, when the [FBPMe]/[SA] ratio was 1: 1, all the drug was bound to HSA, whereas 20-30% of it remained free in the bulk solution in the presence of BSA. The UP approach was also applicable to the study of more complex FBPMe/HSA/BSA mixtures; the obtained results were in good agreement with the previous findings in FBPMe/HSA and FBPMe/BSA systems. This suggests the possibility of making use of the transient triplet-triplet absorption for investigating the distribution of a drug between several compartments in different host biomolecules. | es_ES |
dc.description.sponsorship | Financial support from the MCYT (CTQ2004-03811 and CTQ2007-67010) and the Generalitat Valenciana (GV06/099) is gratefully acknowledged. I.V. thanks MEC for a fellowship. | |
dc.language | Inglés | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation.ispartof | The Journal of Physical Chemistry B | es_ES |
dc.rights | Reserva de todos los derechos | es_ES |
dc.subject.classification | QUIMICA ANALITICA | es_ES |
dc.subject.classification | QUIMICA ORGANICA | es_ES |
dc.title | Transient absorption spectroscopy for determining multiple site occupancy in drug-protein conjugates. A comparison between human and bovine serum albumins using flurbiprofen methyl ester as probe | es_ES |
dc.type | Artículo | es_ES |
dc.identifier.doi | 10.1021/jp076960q | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//CTQ2004-03811/ES/INTERACCIONES FARMACO-BIOMOLECULA EN LOS ESTADOS EXCITADOS/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MEC//CTQ2007-67010/ES/ESPECIES TRANSITORIAS COMO HERRAMIENTAS EN QUIMICA BIOORGANICA/ | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/Generalitat Valenciana//GV06%2F099/ES/ESTADOS EXCITADOS DE FARMACOS COMO SONDAS PARA EL ESTUDIO DE LOS CENTROS ACTIVOS DE PROTEINAS/ | es_ES |
dc.rights.accessRights | Abierto | es_ES |
dc.contributor.affiliation | Universitat Politècnica de València. Departamento de Química - Departament de Química | es_ES |
dc.description.bibliographicCitation | Vayá Pérez, I.; Jiménez Molero, MC.; Miranda Alonso, MÁ. (2008). Transient absorption spectroscopy for determining multiple site occupancy in drug-protein conjugates. A comparison between human and bovine serum albumins using flurbiprofen methyl ester as probe. The Journal of Physical Chemistry B. 112(9):2694-2699. doi:10.1021/jp076960q | es_ES |
dc.description.accrualMethod | S | es_ES |
dc.relation.publisherversion | http://doi.org/10.1021/jp076960q | es_ES |
dc.description.upvformatpinicio | 2694 | es_ES |
dc.description.upvformatpfin | 2699 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | es_ES |
dc.description.volume | 112 | es_ES |
dc.description.issue | 9 | es_ES |
dc.identifier.pmid | 18266356 | |
dc.relation.pasarela | S\33976 | es_ES |
dc.contributor.funder | Generalitat Valenciana | es_ES |
dc.contributor.funder | Ministerio de Educación y Ciencia | es_ES |