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Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants

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Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants

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dc.contributor.author Gramazio, Pietro es_ES
dc.contributor.author Prohens Tomás, Jaime es_ES
dc.contributor.author Borras, Dionís es_ES
dc.contributor.author Plazas Ávila, María de la O es_ES
dc.contributor.author Herraiz García, Francisco Javier es_ES
dc.contributor.author Vilanova Navarro, Santiago es_ES
dc.date.accessioned 2018-05-18T04:23:47Z
dc.date.available 2018-05-18T04:23:47Z
dc.date.issued 2017 es_ES
dc.identifier.issn 0014-2336 es_ES
dc.identifier.uri http://hdl.handle.net/10251/102176
dc.description.abstract [EN] Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers are amongst the most common markers of choice for studies of diversity and relationships in horticultural species. We have used 11 SSR and 35 SNP markers derived from transcriptome sequencing projects to fingerprint 48 accessions of a collection of brinjal (Solanum melongena), gboma (S. macrocarpon) and scarlet (S. aethiopicum) eggplant complexes, which also include their respective wild relatives S. incanum, S. dasyphyllum and S. anguivi. All SSR and SNP markers were polymorphic and 34 and 36 different genetic fingerprints were obtained with SSRs and SNPs, respectively. When combining both markers all accessions but two had different genetic profiles. Although on average SSRs were more informative than SNPs, with a higher number of alleles, genotypes and polymorphic information content (PIC), and expected heterozygosity (He) values, SNPs have proved highly informative in our materials. Low observed heterozygosity (Ho) and high fixation index (f) values confirm the high degree of homozygosity of eggplants. Genetic identities within groups of each complex were higher than with groups of other complexes, although differences in the ranks of genetic identity values among groups were observed between SSR and SNP markers. For low and intermediate values of pair-wise SNP genetic distances, a moderate correlation between SSR and SNP genetic distances was observed (r(2) = 0.592), but for high SNP genetic distances the correlation was low (r(2) = 0.080). The differences among markers resulted in different phenogram topologies, with a different eggplant complex being basal (gboma eggplant for SSRs and brinjal eggplant for SNPs) to the two others. Overall the results reveal that both types of markers are complementary for eggplant fingerprinting and that interpretation of relationships among groups may be greatly affected by the type of marker used. es_ES
dc.description.sponsorship This work has been funded by European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No 677379 (G2P-SOL project: Linking genetic resources, genomes and phenotypes of Solanaceous crops) and by Spanish Ministerio de Economia y Competitividad and Fondo Europeo de Desarrollo Regional (Grant AGL2015-64755-R from MINECO/FEDER). Pietro Gramazio is grateful to Universitat Politecnica de Valencia for a pre-doctoral contract (Programa FPI de la UPV-Subprograma 1/2013 call). Mariola Plazas is grateful to Spanish Ministerio de Economia, Industria y Competitividad for a post-doctoral grant within the Juan de la Cierva-Formacion programme (FJCI-2015-24835). en_EN
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation MINISTERIO DE ECONOMIA INDUSTRIA Y COMPETITIVIDAD /AGL2015-64755-R es_ES
dc.relation COMISION DE LAS COMUNIDADES EUROPEA/677379 es_ES
dc.relation.ispartof Euphytica es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Eggplants es_ES
dc.subject Simple sequence repeats es_ES
dc.subject Single nucleotide polymorphisms es_ES
dc.subject Fingerprinting es_ES
dc.subject Genetic diversity es_ES
dc.subject Cluster analysis es_ES
dc.subject.classification GENETICA es_ES
dc.title Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s10681-017-2057-3 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/677379/EU en_EN
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2018-12-01 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario de Conservación y Mejora de la Agrodiversidad Valenciana - Institut Universitari de Conservació i Millora de l'Agrodiversitat Valenciana es_ES
dc.description.bibliographicCitation Gramazio, P.; Prohens Tomás, J.; Borras, D.; Plazas Ávila, MDLO.; Herraiz García, FJ.; Vilanova Navarro, S. (2017). Comparison of transcriptome-derived simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers for genetic fingerprinting, diversity evaluation, and establishment of relationships in eggplants. Euphytica. 213(264):1-18. doi:10.1007/s10681-017-2057-3 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s10681-017-2057-3 es_ES
dc.description.upvformatpinicio 1 es_ES
dc.description.upvformatpfin 18 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 213 es_ES
dc.description.issue 264 es_ES
dc.relation.pasarela S\347960 es_ES
dc.contributor.funder European Commission es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
dc.relation.references Acquadro A, Barchi L, Gramazio P et al (2017) Coding SNPs analysis highlights genetic relationships and evolution pattern in eggplant complexes. PLoS ONE 12:e0180774. https://doi.org/10.1371/journal.pone.0180774 es_ES
dc.relation.references Adeniji O, Kusolwa P, Reuben S (2013) Morphological descriptors and micro satellite diversity among scarlet eggplant groups. Afr Crop Sci J 21(1):37–49 es_ES
dc.relation.references Aguoru C, Omoigui L, Olasan J (2015) Molecular characterization of Solanum species (Solanum aethiopicum complex; Solanum macrocarpon and Solanum anguivi) using multiplex RAPD primers. J Plant Stud 4:27–34. https://doi.org/10.5539/jps.v4n1p27 es_ES
dc.relation.references Arumuganathan K, Earle E (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9(3):208–218 es_ES
dc.relation.references Ashrafi H, Hill T, Stoffel K et al (2012) De novo assembly of the pepper transcriptome (Capsicum annuum): a benchmark for in silico discovery of SNPs, SSRs and candidate genes. BMC Genom 13:1–15. https://doi.org/10.1186/1471-2164-13-571 es_ES
dc.relation.references Augustinos AA, Petropoulos C, Karasoulou V et al (2016) Assessing diversity among traditional Greek and foreign eggplant cultivars using molecular markers and morphometrical descriptors. Span J Agric Res 14:e0710. https://doi.org/10.5424/sjar/2016144-9020 es_ES
dc.relation.references Avise JC (2012) Molecular markers, natural history and evolution. Springer Science & Business Media, Berlin. https://doi.org/10.1007/978-1-4615-2381-9 es_ES
dc.relation.references Blanca J, Cañizares J, Roig C et al (2011) Transcriptome characterization and high throughput SSRs and SNPs discovery in Cucurbita pepo (Cucurbitaceae). BMC Genom 12:104. https://doi.org/10.1186/1471-2164-12-104 es_ES
dc.relation.references Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32(3):314–331 es_ES
dc.relation.references Bukenya Z, Carasco J (1994) Biosystematic study of Solanum macrocarpon—S. dasyphyllum complex in Uganda and relations with Solanum linnaeanum. East Afr Agric For J 59(3):187–204 es_ES
dc.relation.references Castillo A, Budak H, Varshney RK et al (2008) Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense. BMC Plant Biol 8:97. https://doi.org/10.1186/1471-2229-8-97 es_ES
dc.relation.references Choudhary S, Sethy NK, Shokeen B, Bhatia S (2009) Development of chickpea EST-SSR markers and analysis of allelic variation across related species. Theor Appl Genet 118:591–608. https://doi.org/10.1007/s00122-008-0923-z es_ES
dc.relation.references Coates BS, Sumerford DV, Miller NJ et al (2009) Comparative performance of single nucleotide polymorphism and microsatellite markers for population genetic analysis. J Hered 100:556–564. https://doi.org/10.1093/jhered/esp028 es_ES
dc.relation.references D’Agostino N, Golas T, van de Geest H et al (2013) Genomic analysis of the native European Solanum species, S. dulcamara. BMC Genom 14:356. https://doi.org/10.1186/1471-2164-14-356 es_ES
dc.relation.references Daunay MC, Hazra P (2012) Eggplant. In: Peter KV, Hazra P (eds) Handbook of Vegetables. Studium Press, Houston, pp 257–322 es_ES
dc.relation.references Davey J, Hohenlohe P, Etter P et al (2011) Genome-wide genetic marker discovery and genotyping using next-generation sequencing. Nat Rev Genet 12:499–510. https://doi.org/10.1038/nrg3012 es_ES
dc.relation.references De Barba M, Miquel C, Lobréaux S et al (2016) High-throughput microsatellite genotyping in ecology: improved accuracy, efficiency, standardization and success with low-quantity and degraded DNA. Mol Ecol Resour 17(3):492–507. https://doi.org/10.1111/1755-0998.12594 es_ES
dc.relation.references Doyle J, Doyle J (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15 es_ES
dc.relation.references Ellegren H (2004) Microsatellites: simple sequences with complex evolution. Nat Rev Genet 5:435–445. https://doi.org/10.1038/nrg1348 es_ES
dc.relation.references Felsenstein, J (2007). PHYLIP (Phylogeny Inference Package) Version 3.67. Department of Genome Sciences, University of Washington, Seattle, WA, USA es_ES
dc.relation.references Fernandez-Silva I, Whitney J, Wainwright B (2013) Microsatellites for next-generation ecologists: a post-sequencing bioinformatics pipeline. PLoS ONE 8(2):e55990 es_ES
dc.relation.references Filippi CV, Aguirre N, Rivas JG et al (2015) Population structure and genetic diversity characterization of a sunflower association mapping population using SSR and SNP markers. BMC Plant Biol 15:52. https://doi.org/10.1186/s12870-014-0360-x es_ES
dc.relation.references Fischer MC, Rellstab C, Leuzinger M et al (2017) Estimating genomic diversity and population differentiation—an empirical comparison of microsatellite and SNP variation in Arabidopsis halleri. BMC Genom 18:69. https://doi.org/10.1186/s12864-016-3459-7 es_ES
dc.relation.references Furini A, Wunder J (2004) Analysis of eggplant (Solanum melongena)-related germplasm: morphological and AFLP data contribute to phylogenetic interpretations and germplasm utilization. Theor Appl Genet 108:197–208. https://doi.org/10.1007/s00122-003-1439-1 es_ES
dc.relation.references Gadaleta A, Giancaspro A, Zacheo S et al (2011) Comparison of genomic and EST-derived SSR markers in phylogenetic analysis of wheat. Plant Genet Resour 9:243–246. https://doi.org/10.1017/S147926211100030X es_ES
dc.relation.references Ge H, Liu Y, Jiang M et al (2013) Analysis of genetic diversity and structure of eggplant populations (Solanum melongena L.) in China using simple sequence repeat markers. Sci Hortic 162:71–75. https://doi.org/10.1016/j.scienta.2013.08.004 es_ES
dc.relation.references Gonzaga ZJ (2015) Evaluation of SSR and SNP Markers for Molecular Breeding in Rice. Plant Breed Biotechnol 3:139–152. https://doi.org/10.9787/PBB.2015.3.2.139 es_ES
dc.relation.references Goodwin S, McPherson J, McCombie W (2016) Coming of age: ten years of next-generation sequencing technologies. Nat Rev Genet 17(6):333–351 es_ES
dc.relation.references Gramazio P, Blanca J, Ziarsolo P et al (2016) Transcriptome analysis and molecular marker discovery in Solanum incanum and S. aethiopicum, two close relatives of the common eggplant (Solanum melongena) with interest for breeding. BMC Genom 17:300. https://doi.org/10.1186/s12864-016-2631-4 es_ES
dc.relation.references Grover A, Sharma PC (2014) Development and use of molecular markers: past and present. Crit Rev Biotechnol 8551:1–13. https://doi.org/10.3109/07388551.2014.959891 es_ES
dc.relation.references Hamblin MT, Warburton ML, Buckler ES (2007) Empirical comparison of simple sequence repeats and single nucleotide polymorphisms in assessment of maize diversity and relatedness. PLoS ONE 2:e1367. https://doi.org/10.1371/journal.pone.0001367 es_ES
dc.relation.references Hess JE, Matala AP (2011) Comparison of SNPs and microsatellites for fine-scale application of genetic stock identification of Chinook salmon in the Columbia River Basin Comparison of SNPs and microsatellites for fine-scale application of genetic stock identification of Chinook salmon in the Columbia River Basin. Mol Ecol Resour. https://doi.org/10.1111/j.1755-0998.2010.02958.x es_ES
dc.relation.references Highton R (1993) The relationship between the number of loci and the statistical support for the topology of UPGMA trees obtained from genetic distance data. Mol Phylogenet Evol 2:337–343 es_ES
dc.relation.references Hirakawa H, Shirasawa K, Miyatake K, Nunome, T et al (2014) Draft genome sequence of eggplant (Solanum melongena L.): the representative solanum species indigenous to the old world. DNA Res 21:649–660. https://doi.org/10.1093/dnares/dsu027 es_ES
dc.relation.references Hong CP, Piao ZY, Kang TW et al (2007) Genomic distribution of simple sequence repeats in Brassica rapa. Mol Cells 23:349–356. es_ES
dc.relation.references Hu J, Wang L, Li J (2011) Comparison of genomic SSR and EST-SSR markers for estimating genetic diversity in cucumber. Biol Plant 55:577–580. https://doi.org/10.1007/s10535-011-0129-0 es_ES
dc.relation.references Isshiki S, Iwata N, Khan MMR (2008) ISSR variations in eggplant (Solanum melongena L.) and related Solanum species. Sci Hortic 117:186–190. https://doi.org/10.1016/j.scienta.2008.04.003 es_ES
dc.relation.references Jones ES, Sullivan H, Bhattramakki D, Smith JSC (2007) A comparison of simple sequence repeat and single nucleotide polymorphism marker technologies for the genotypic analysis of maize (Zea mays L.). Theor Appl Genet 115:361–371. https://doi.org/10.1007/s00122-007-0570-9 es_ES
dc.relation.references Kalia RK, Rai MK, Kalia S et al (2011) Microsatellite markers: an overview of the recent progress in plants. Euphytica 177:309–334 es_ES
dc.relation.references Kashi Y, King DG (2006) Simple sequence repeats as advantageous mutators in evolution. Trends Genet 22:253–259. https://doi.org/10.1016/j.tig.2006.03.005 es_ES
dc.relation.references Kaushik P, Prohens J, Vilanova S et al (2016) Phenotyping of eggplant wild relatives and interspecific hybrids with conventional and phenomics descriptors provides insight for their potential utilization in breeding. Front Plant Sci 7:677 es_ES
dc.relation.references Kim C, Guo H, Kong W et al (2016) Application of genotyping by sequencing technology to a variety of crop breeding programs. Plant Sci 242:14–22 es_ES
dc.relation.references Knapp S, Vorontsova MS, Prohens J (2013) Wild relatives of the eggplant (Solanum melongena L.: Solanaceae): new understanding of species names in a complex group. PLoS ONE 8:e57039 es_ES
dc.relation.references Kruglyak S, Durrett RT, Schug MD, Aquadro CF (1998) Equilibrium distributions of microsatellite repeat length resulting from a balance between slippage events and point mutations. Proc Natl Acad Sci USA 95:10774–10778. https://doi.org/10.1073/pnas.95.18.10774 es_ES
dc.relation.references Lester RN, Daunay MC (2003) Diversity of African vegetable Solanum species and its implications for a better understanding of plant domestication. Schriften zu Genetischen Ressourcen 22:137–152 es_ES
dc.relation.references Lester RN, Niakan L (1986) Origin and domestication of the scarlet eggplant, Solanum aethiopicum, from S. anguivi in Africa. In: D’Arcy WG (ed) Solanaceae: biology and systematics. Columbia University Press, New York, pp 433–456 es_ES
dc.relation.references Lester RN, Jaeger PML, Bleijendaal-Spierings BHM et al (1990) African eggplants-a review of collecting in West Africa. Plant Genet Resour Newsl 81:17–26 es_ES
dc.relation.references Levin R, Myers N, Bohs L (2006) Phylogenetic relationships among the ‘spiny solanums’ (Solanum subgenus Leptostemonum, Solanaceae). Am J Bot 93(1):157–169 es_ES
dc.relation.references Li WH, Gojobori T, Nei M (1981) Pseudogenes as a paradigm of neutral evolution. Nature 292:237–239 es_ES
dc.relation.references Li YC, Korol AB, Fahima T et al (2002) Microsatellites: genomic distribution, putative functions and mutational mechanisms: a review. Mol Ecol 11:2453–2465 es_ES
dc.relation.references Liu K, Muse S (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129 es_ES
dc.relation.references Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220. https://doi.org/10.1038/214637b0 es_ES
dc.relation.references Martínez-Arias R, Calafell F, Mateu E et al (2001) Sequence variability of a human pseudogene. Genome Res 11:1071–1085. https://doi.org/10.1101/gr.167701 es_ES
dc.relation.references Meyer RS, Karol KG, Little DP et al (2012) Phylogeographic relationships among Asian eggplants and new perspectives on eggplant domestication. Mol Phylogenet Evol 63:685–701. https://doi.org/10.1016/j.ympev.2012.02.006 es_ES
dc.relation.references Muñoz-Falcón J, Prohens J, Vilanova S, Nuez F (2009) Diversity in commercial varieties and landraces of black eggplants and implications for broadening the breeders’ gene pool. Ann Appl Biol 154(3):453–465 es_ES
dc.relation.references Nandha PS, Singh J (2014) Comparative assessment of genetic diversity between wild and cultivated barley using gSSR and EST-SSR markers. Plant Breed 133:28–35. https://doi.org/10.1111/pbr.12118 es_ES
dc.relation.references Nei M (1972) Genetic distance between populations. Am Nat 106:283–292. https://doi.org/10.1086/282771 es_ES
dc.relation.references Nunome T, Negoro S, Kono I et al (2009) Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor Appl Genet 119:1143–1153. https://doi.org/10.1007/s00122-009-1116-0 es_ES
dc.relation.references Page R (2001) TreeView. Glasgow University, Glasgow es_ES
dc.relation.references Peakall P, Smouse R (2012) GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research an update. Bioinformatics 28:2537–2539 es_ES
dc.relation.references Pessarakli M, Dris R (2004) Pollination and breeding of eggplants. J Food Agric Environ 2:218–219 es_ES
dc.relation.references Plazas M, Andújar I, Vilanova S et al (2014) Conventional and phenomics characterization provides insight into the diversity and relationships of hypervariable scarlet (Solanum aethiopicum L.) and gboma (S. macrocarpon L.) eggplant complexes. Front. Plant Sci 5:318 es_ES
dc.relation.references Ranil R, Niran H, Plazas M et al (2015) Improving seed germination of the eggplant rootstock Solanum torvum by testing multiple factors using an orthogonal array design. Sci Hortic 193:174–181. https://doi.org/10.1016/j.scienta.2015.07.030 es_ES
dc.relation.references Sakata Y, Lester RN (1997) Chloroplast DNA diversity in brinjal eggplant (Solanum melongena L.) and related species. Euphytica 97:295–301. https://doi.org/10.1023/A:1003000612441 es_ES
dc.relation.references Sakata Y, Nishio T, Matthews PJ (1991) Chloroplast DNA analysis of eggplant (Solanum melongena) and related species for their taxonomic affinity. Euphytica 55:21–26 es_ES
dc.relation.references Särkinen T, Bohs L, Olmstead RG, Knapp S (2013) A phylogenetic framework for evolutionary study of the nightshades (Solanaceae): a dated 1000-tip tree. BMC Evol Biol 13:214. https://doi.org/10.1186/1471-2148-13-214 es_ES
dc.relation.references Scheben A, Batley J, Edwards D (2017) Genotyping-by-sequencing approaches to characterize crop genomes: choosing the right tool for the right application. Plant Biotechnol J 15:149–161 es_ES
dc.relation.references Sneath P, Sokal R (1973) Numerical taxonomy. The principles and practice of numerical classification. W H Freeman Limited, San Francisco es_ES
dc.relation.references Stàgel A, Portis E, Toppino L et al (2008) Gene-based microsatellite development for mapping and phylogeny studies in eggplant. BMC Genom 9:357. https://doi.org/10.1186/1471-2164-9-357 es_ES
dc.relation.references Sunseri F, Polignano GB, Alba V et al (2010) Genetic diversity and characterization of African eggplant germplasm collection. Afr J Plant Sci 4:231–241 es_ES
dc.relation.references Syfert MM, Castañeda-Álvarez NP, Khoury CK et al (2016) Crop wild relatives of the brinjal eggplant (Solanum melongena): poorly represented in genebanks and many species at risk of extinction. Am J Bot 103:635–651. https://doi.org/10.3732/ajb.1500539 es_ES
dc.relation.references Thiel T, Michalek W, Varshney R, Graner A (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L.). Theor Appl Genet 106:411–422. https://doi.org/10.1007/s00122-002-1031-0 es_ES
dc.relation.references Thomson MJ, Alfred J, Dangl J et al (2014) High-throughput SNP genotyping to accelerate crop improvement. Plant Breed Biotechnol 2:195–212. https://doi.org/10.9787/PBB.2014.2.3.195 es_ES
dc.relation.references Thorvaldsdóttir H, Robinson JT, Mesirov JP (2013) Integrative genomics viewer (IGV): high-performance genomics data visualization and exploration. Brief Bioinform 14:178–192. https://doi.org/10.1093/bib/bbs017 es_ES
dc.relation.references Tumbilen Y, Frary A, Daunay MC, Doganlar S (2011) Application of EST-SSRs to examine genetic diversity in eggplant and its close relatives. Turk J Biol 35:125–136. https://doi.org/10.3906/biy-0906-57 es_ES
dc.relation.references van Inghelandt D, Melchinger AE, Lebreton C, Stich B (2010) Population structure and genetic diversity in a commercial maize breeding program assessed with SSR and SNP markers. Theor Appl Genet 120:1289–1299. https://doi.org/10.1007/s00122-009-1256-2 es_ES
dc.relation.references Van Tassell CP, Smith TPL, Matukumalli LK et al (2008) SNP discovery and allele frequency estimation by deep sequencing of reduced representation libraries. Nat Methods 5:247–252. https://doi.org/10.1038/nmeth.1185 es_ES
dc.relation.references Varshney R, Graner A, Sorrells M (2005) Genic microsatellite markers in plants: features and applications. Trends Biotechnol 23(1):48–55 es_ES
dc.relation.references Varshney RK, Chabane K, Hendre PS et al (2007) Comparative assessment of EST-SSR, EST-SNP and AFLP markers for evaluation of genetic diversity and conservation of genetic resources using wild, cultivated and elite barleys. Plant Sci 173:638–649. https://doi.org/10.1016/j.plantsci.2007.08.010 es_ES
dc.relation.references Vilanova S, Manzur JP, Prohens J (2012) Development and characterization of genomic simple sequence repeat markers in eggplant and their application to the study of diversity and relationships in a collection of different cultivar types and origins. Mol Breed 30:647–660. https://doi.org/10.1007/s11032-011-9650-2 es_ES
dc.relation.references Vilanova S, Hurtado M, Cardona A (2014) Genetic diversity and relationships in local varieties of eggplant from different cultivar groups as assessed by genomic SSR markers. Not Bot Horti Agrobo Cluj-Napoca 42:59–65 es_ES
dc.relation.references Vogel JP, Gu YQ, Twigg P et al (2006) EST sequencing and phylogenetic analysis of the model grass Brachypodium distachyon. Theor Appl Genet 113:186–195. https://doi.org/10.1007/s00122-006-0285-3 es_ES
dc.relation.references Vorontsova MS, Stern S, Bohs L, Knapp S (2013) African spiny solanum (subgenus Leptostemonum, Solanaceae): a thorny phylogenetic tangle. Bot J Linn Soc 173:176–193. https://doi.org/10.1111/boj.12053 es_ES
dc.relation.references Weese TL, Bohs L (2010) Eggplant origins: out of Africa, into the Orient. Taxon 59:49–56. https://doi.org/10.2307/27757050 es_ES
dc.relation.references Wright S (1965) The interpretation of population structure by F-statistics with special regard to systems of mating. Evolution 19:395–420. https://doi.org/10.2307/2406450 es_ES
dc.relation.references Xiao M, Zhang Y, Chen X et al (2013) Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest. J Biotechnol 166:122–134. https://doi.org/10.1016/j.jbiotec.2013.04.004 es_ES
dc.relation.references Yan J, Yang X, Shah T et al (2010) High-throughput SNP genotyping with the Goldengate assay in maize. Mol Breed 25:441–451. https://doi.org/10.1007/s11032-009-9343-2 es_ES
dc.relation.references Yang X, Xu Y, Shah T et al (2011) Comparison of SSRs and SNPs in assessment of genetic relatedness in maize. Genetica 139:1045–1054. https://doi.org/10.1007/s10709-011-9606-9 es_ES
dc.relation.references Yu J, Zhang Z, Zhu C et al (2009) Simulation appraisal of the adequacy of number of background markers for relationship estimation in association mapping. Plant Genome 2:63. https://doi.org/10.3835/plantgenome2008.09.0009 es_ES
dc.relation.references Zhan L, Paterson I, Fraser B (2016) MEGASAT: automated inference of microsatellite genotypes from sequence data. Ecol Resour, Mol. https://doi.org/10.1111/1755-0998.12561 es_ES


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