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Simultaneous determination of four food allergens using compact disc immunoassaying technology

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Simultaneous determination of four food allergens using compact disc immunoassaying technology

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dc.contributor.author Ali Badran, Ahmed es_ES
dc.contributor.author Morais, Sergi es_ES
dc.contributor.author Maquieira Catala, Ángel es_ES
dc.date.accessioned 2018-05-21T04:29:04Z
dc.date.available 2018-05-21T04:29:04Z
dc.date.issued 2017 es_ES
dc.identifier.issn 1618-2642 es_ES
dc.identifier.uri http://hdl.handle.net/10251/102331
dc.description.abstract [EN] A multiplex competitive microimmunoassay for the simultaneous determination of gliadin, casein, β-lactoglobulin, and ovalbumin is presented. The assay in microarray format is performed on a DVD where the allergens are physisorbed on the polycarbonate surface of the disc. The immunointeraction is detected using a mixture of specific gold-labeled antibodies and the signal amplified with the silver enhancement method. The optical density of the precipitate, read by a DVD drive, is related to the concentration of the four allergens in sample. An optimized protocol for the simultaneous extraction of the allergen proteins from food samples is also addressed. The suitability of the method is demonstrated for the simultaneous quantitative extraction and determination of the targeted allergens in spiked baby foods, juices, and beers. The sensitivity (EC50) of the multiplexed assay was 0.04, 0.40, 0.08, and 0.16 mg L-1 for gliadin, casein, β-lactoglobulin, and ovalbumin, respectively, and the recovery results from the analysis of food samples ranged from 72 to 117%. A portable, easy-to-use, array-based bioanalytical method is developed for quantification of food allergens with a limit of detection below the accepted levels of the international legislations, which allows promotion of food safety and quality. es_ES
dc.description.sponsorship This research was granted by the Spanish Ministry of Economy and Competitiveness (CTQ2013-45875-R) and Generalitat Valenciana (PROMETEO II 2014/040) by FEDER funds. Thanks to Ingenasa, S.A. (Madrid, Spain) for providing antibodies and reference materials. A. Ali gratefully acknowledges the fellowship (Programa Santiago Grisolía: 2013/040) funded by Generalitat Valenciana.
dc.language Inglés es_ES
dc.publisher Springer-Verlag es_ES
dc.relation.ispartof Analytical and Bioanalytical Chemistry es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Food allergy es_ES
dc.subject Gliadin es_ES
dc.subject lactoglobulin es_ES
dc.subject Casein es_ES
dc.subject Ovalbumin es_ES
dc.subject Simultaneous extraction multiplex competitive microimmunoassay es_ES
dc.subject Compact disc microarraying es_ES
dc.subject.classification QUIMICA ANALITICA es_ES
dc.title Simultaneous determination of four food allergens using compact disc immunoassaying technology es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1007/s00216-016-0170-0 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/MINECO//CTQ2013-45875-R/ES/BIOSENSADO EN SOPORTES INTERACTIVOS CON PROPIEDADES INTERFEROMETRICAS PARA APLICACIONES CLINICAS. DEMOSTRACION EN FARMACOGENETICA Y ALERGIAS MEDICAMENTOSAS/ es_ES
dc.relation.projectID info:eu-repo/grantAgreement/GVA//PROMETEOII%2F2014%2F040/ES/Estudio de estrategias fisico-químicas para el desarrollo de biosensores interferométricos en soportes interactivos de aplicación en clínica/ es_ES
dc.rights.accessRights Cerrado es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Química - Departament de Química es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto de Reconocimiento Molecular y Desarrollo Tecnológico - Institut de Reconeixement Molecular i Desenvolupament Tecnològic es_ES
dc.description.bibliographicCitation Ali Badran, A.; Morais, S.; Maquieira Catala, Á. (2017). Simultaneous determination of four food allergens using compact disc immunoassaying technology. Analytical and Bioanalytical Chemistry. 409:2261-2268. https://doi.org/10.1007/s00216-016-0170-0 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1007/s00216-016-0170-0 es_ES
dc.description.upvformatpinicio 2261 es_ES
dc.description.upvformatpfin 2268 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 409 es_ES
dc.relation.pasarela S\327276 es_ES
dc.contributor.funder Generalitat Valenciana es_ES
dc.contributor.funder Ministerio de Economía, Industria y Competitividad es_ES
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