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ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor

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ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor

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dc.contributor.author RIOS, P es_ES
dc.contributor.author Argyris, J es_ES
dc.contributor.author VEGAS, J es_ES
dc.contributor.author LEIDA, C es_ES
dc.contributor.author Kenigswald, M es_ES
dc.contributor.author Tzuri, G es_ES
dc.contributor.author Troadec, C es_ES
dc.contributor.author Bendahmane, A es_ES
dc.contributor.author KATZIR, NURIT es_ES
dc.contributor.author Picó Sirvent, María Belén es_ES
dc.contributor.author Monforte Gilabert, Antonio José es_ES
dc.contributor.author GARCÍA-MAS, J es_ES
dc.date.accessioned 2018-05-26T04:24:12Z
dc.date.available 2018-05-26T04:24:12Z
dc.date.issued 2017 es_ES
dc.identifier.issn 0960-7412 es_ES
dc.identifier.uri http://hdl.handle.net/10251/102701
dc.description.abstract [EN] Fruit ripening is divided into climacteric and non-climacteric types depending on the presence or absence of a transient rise in respiration rate and the production of autocatalytic ethylene. Melon is ideal for the study of fruit ripening, as both climacteric and non-climacteric varieties exist. Two introgressions of the non-climacteric accession PI 161375, encompassed in the QTLs ETHQB3.5 and ETHQV6.3, into the non-climacteric 'Piel de Sapo' background are able to induce climacteric ripening independently. We report that the gene underlying ETHQV6.3 is MELO3C016540 (CmNAC-NOR), encoding a NAC ((N) under bar AM, (A) under bar TAF1,2, (C) under bar UC2) transcription factor that is closely related to the tomato NOR (non-ripening) gene. CmNAC-NOR was functionally validated through the identification of two TILLING lines carrying non-synonymous mutations in the conserved NAC domain region. In an otherwise highly climacteric genetic background, both mutations provoked a significant delay in the onset of fruit ripening and in the biosynthesis of ethylene. The PI 161375 allele of ETHQV6.3 is similar to that of climacteric lines of the cantalupensis type and, when introgressed into the non-climacteric 'Piel de Sapo', partially restores its climacteric ripening capacity. CmNAC-NOR is expressed in fruit flesh of both climacteric and non-climacteric lines, suggesting that the causal mutation may not be acting at the transcriptional level. The use of a comparative genetic approach in a species with both climacteric and non-climacteric ripening is a powerful strategy to dissect the complex mechanisms regulating the onset of fruit ripening. es_ES
dc.description.sponsorship The authors thank Dr J. Burger for providing the NAxDul population, Dr E. Falik for ethylene analysis of this population, and both for their critical inputs. This work was supported by the Spanish Ministry of Economy and Competitivity grant AGL2015-64625-C2-1-R, Centro de Excelencia Severo Ochoa 2016-2020, and the CERCA Programme/Generalitat de Catalunya to JGM; Spanish Ministry of Economy and Competitivity/FEDER grant AGL2015-64625-C2-2-R to AJM; EU Framework Program Horizon 2020 COST Action FA1106 Quality Fruit for networking activities to CL; European Research Council grant ERC-SEXYPARTH to AB; Chief Scientist of the Ministry of Agriculture of Israel grant no. 261-1049-13 to NK. es_ES
dc.language Inglés es_ES
dc.publisher Blackwell Publishing es_ES
dc.relation MINISTERIO DE ECONOMIA Y COMPETITIVIDAD/AGL2015-64625-C2-2-R (Subproyecto Plan Estatal) es_ES
dc.relation.ispartof The Plant Journal es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject Non-climacteric ripening es_ES
dc.subject ETHQV6.3 es_ES
dc.subject NAC transcription factor es_ES
dc.subject TILLING mutant es_ES
dc.subject Cucumis melo es_ES
dc.subject Introgression line es_ES
dc.subject.classification GENETICA es_ES
dc.title ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1111/tpj.13596 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/341076/EU en_EN
dc.rights.accessRights Abierto es_ES
dc.contributor.affiliation Universitat Politècnica de València. Instituto Universitario Mixto de Biología Molecular y Celular de Plantas - Institut Universitari Mixt de Biologia Molecular i Cel·lular de Plantes es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Biotecnología - Departament de Biotecnologia es_ES
dc.description.bibliographicCitation Rios, P.; Argyris, J.; Vegas, J.; Leida, C.; Kenigswald, M.; Tzuri, G.; Troadec, C.... (2017). ETHQV6.3 is involved in melon climacteric fruit ripening and is encoded by a NAC domain transcription factor. The Plant Journal. 91(4):671-683. https://doi.org/10.1111/tpj.13596 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion https://doi.org/10.1111/tpj.13596 es_ES
dc.description.upvformatpinicio 671 es_ES
dc.description.upvformatpfin 683 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 91 es_ES
dc.description.issue 4 es_ES
dc.identifier.pmid 28493311 en_EN
dc.relation.pasarela S\346311 es_ES
dc.contributor.funder Ministerio de Economía y Competitividad es_ES


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