- -

Confined Sandwichlike Microenvironments Tune Myogenic Differentiation

RiuNet: Institutional repository of the Polithecnic University of Valencia

Share/Send to

Cited by

Statistics

Confined Sandwichlike Microenvironments Tune Myogenic Differentiation

Show simple item record

Files in this item

dc.contributor.author Ballester Beltrán, José es_ES
dc.contributor.author Trujillo, Sara es_ES
dc.contributor.author Alakpa, Enateri V. es_ES
dc.contributor.author Compañ Moreno, Vicente es_ES
dc.contributor.author Gavara, Rafael es_ES
dc.contributor.author Meek, Dominic es_ES
dc.contributor.author West, Christopher C. es_ES
dc.contributor.author Peault, Bruno es_ES
dc.contributor.author Dalby, Matthew J. es_ES
dc.contributor.author Salmerón Sánchez, Manuel es_ES
dc.date.accessioned 2018-06-03T04:27:22Z
dc.date.available 2018-06-03T04:27:22Z
dc.date.issued 2017 es_ES
dc.identifier.uri http://hdl.handle.net/10251/103267
dc.description.abstract [EN] Sandwichlike (SW) cultures are engineered as a multilayer technology to simultaneously stimulate dorsal and ventral cell receptors, seeking to mimic cell adhesion in three-dimensional (3D) environments in a reductionist manner. The effect of this environment on cell differentiation was investigated for several cell types cultured in standard growth media, which promotes proliferation on two-dimensional (2D) surfaces and avoids any preferential differentiation. First, murine C2C12 myoblasts showed specific myogenic differentiation. Human mesenchymal stem cells (hMSCs) of adipose and bone marrow origin, which can differentiate toward a wider variety of lineages, showed again myodifferentiation. Overall, this study shows myogenic differentiation in normal growth media for several cell types under SW conditions, avoiding the use of growth factors and cytokines, i.e., solely by culturing cells within the SW environment. Mechanistically, it provides further insights into the balance between integrin adhesion to the dorsal substrate and the confinement imposed by the SW system. es_ES
dc.description.sponsorship The support of ERC through HealInSynergy (306990), EPSRC (EP/P001114/1) and FPU program AP2009-3626 are acknowledged. en_EN
dc.language Inglés es_ES
dc.publisher American Chemical Society es_ES
dc.relation.ispartof ACS Biomaterials Science & Engineering es_ES
dc.rights Reserva de todos los derechos es_ES
dc.subject 3D culture es_ES
dc.subject Confinement es_ES
dc.subject Myodifferentiation es_ES
dc.subject C2C12 es_ES
dc.subject hMSC es_ES
dc.subject.classification TERMODINAMICA APLICADA (UPV) es_ES
dc.subject.classification FISICA APLICADA es_ES
dc.subject.classification MAQUINAS Y MOTORES TERMICOS es_ES
dc.title Confined Sandwichlike Microenvironments Tune Myogenic Differentiation es_ES
dc.type Artículo es_ES
dc.identifier.doi 10.1021/acsbiomaterials.7b00109 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/ME//AP2009-3626/ES/AP2009-3626/ / es_ES
dc.relation.projectID info:eu-repo/grantAgreement/RCUK/EPSRC/EP/P001114/1/GB/ en_EN
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/306990/EU/Material-driven Fibronectin Fibrillogenesis to Engineer Synergistic Growth Factor Microenvironments/
dc.rights.accessRights Abierto es_ES
dc.date.embargoEndDate 2018-08-31 es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Física Aplicada - Departament de Física Aplicada es_ES
dc.contributor.affiliation Universitat Politècnica de València. Departamento de Termodinámica Aplicada - Departament de Termodinàmica Aplicada es_ES
dc.description.bibliographicCitation Ballester Beltrán, J.; Trujillo, S.; Alakpa, EV.; Compañ Moreno, V.; Gavara, R.; Meek, D.; West, CC.... (2017). Confined Sandwichlike Microenvironments Tune Myogenic Differentiation. ACS Biomaterials Science & Engineering. 3(8):1710-1718. https://doi.org/10.1021/acsbiomaterials.7b00109 es_ES
dc.description.accrualMethod S es_ES
dc.relation.publisherversion http://dx.doi.org/10.1021/acsbiomaterials.7b00109 es_ES
dc.description.upvformatpinicio 1710 es_ES
dc.description.upvformatpfin 1718 es_ES
dc.type.version info:eu-repo/semantics/publishedVersion es_ES
dc.description.volume 3 es_ES
dc.description.issue 8 es_ES
dc.identifier.eissn 2373-9878 es_ES
dc.identifier.pmid 28824958 en_EN
dc.identifier.pmcid PMC5558191 en_EN
dc.relation.pasarela S\351347 es_ES
dc.contributor.funder Ministerio de Educación es_ES


This item appears in the following Collection(s)

Show simple item record